功率谱密度法标定光镊的三维光阱刚度

采用四象限探测器和功率谱密度法,搭建了一套快速标定光镊三维光阱刚度的测量系统.实验中,用四象限探测器记录微粒做受限布朗运动时的位置信息,用功率谱密度法标定光阱刚度,测得了直径0.97μm SiO2小球和直径1μm PMMA小球的光阱刚度与激光功率的关系.结果表明:对于SiO2小球,当激光功率为50~120mW时,光阱刚度与激光功率成正比;对于PMMA小球,当激光功率为80~130mW时,光阱刚度与激光功率成正比.该光镊系统可用于生物、物理等微观领域研究的高准确度测力系统.     

时间飞行法测量光阱刚度的实验研究

传统的测量光阱刚度的方法如功率谱法是基于微粒的布朗运动,适用于直径范围几百纳米到几微米的微球,在几微米以上并不具有明显优势．本文发展一种时间飞行的方法测量光阱对微球的刚度．该方法是基于跟踪微粒的运动轨迹获得光阱刚度．通过比较不同功率下,不同大小以及不同材料的微球的光阱刚度和误差,结果表明时间飞行法适用于直径范围5—10μm的微球;论文中用功率谱法和均方位移法测量了5μm标准聚苯乙烯小球的光阱刚度与时间飞行法测得的结果作为对比,由于受相机采集速率的影响,所测刚度值比理想值偏高,比较而言,时间飞行法的测量结果更加接近于真实值,对于光阱刚度的快速标定有着重要意义．该方法可以应用在特殊光场分布的激光阱中测量微球的光阱刚度;在实现细胞层次的力学特性测量中它可避免使用微球作为探针,为更深层次研究细胞上的复杂单分子过程提供了一个研究手段．     

时域有限差分法数值仿真单光镊中微球受到的光阱力

本文采用三维时域有限差分法(FDTD)和Maxwell应力张量法建立了单光镊在焦点附近俘获球形微粒的光阱力模型,采用基于球矢量波函数(VSWF)的五阶高斯光源作为仿真光源,得到了准确的光场传播.讨论了光源的波长、束腰、偏振态和微球的半径、折射率对光阱力的影响,分析了在单光镊俘获微球时,邻近微球对光阱力的影响.特别研究了光源的偏振态对微球所受光阱力的作用效果,仿真结果表明圆偏振光比线偏振光对微球的俘获力更大;被光镊稳定俘获的微球,会受到邻近微球干扰,失去平衡状态,改变光源的偏振态可以改变微球的受力状态. 关键词： 光镊 光阱力 介质微球 时域有限差分法(FDTD)     

轴向多光阱微粒捕获与实时直接观测技术

传统的光镊技术使用单个物镜同时进行光学捕获与显微成像,使得捕获与成像区域被限制在物镜焦平面附近,无法同时观察到沿光轴方向(即Z向)捕获的多个微粒.本文提出一种轴平面(XZ平面)GerchbergSaxton迭代算法来产生沿轴向分布的多光阱阵列,将轴平面成像技术与光镊结合,实现了沿轴向对二氧化硅微球的多光阱同时捕获与实时观测.通过视频分析法测量了多个二氧化硅微球在轴向光镊阵列中的布朗运动,并标定了光阱刚度.本文提出的轴向多光阱微粒捕获与实时观测技术为光学微操纵提供了一个新的观测视角和操纵方法,为生物医学、物理学等相关领域研究提供了一种新的技术手段.     

光子晶体光纤中超连续谱产生的理论与实验研究

研究了光子晶体光纤中超连续激光光源的产生机理.利用非线性偏振旋转技术产生的中心波长为1 556.0 nm的飞秒光脉冲作为泵浦光源,在69 m长的高非线性光子晶体光纤中,得到了20 dB带宽约为140 nm的超连续谱;采用实验和数值模拟方法,研究了不同泵浦功率下超连续谱形成的过程.结果表明,在不同的泵浦功率下,超连续谱的形成机理不同,在各种非线性效应的共同作用下,泵浦光脉冲的峰值功率越高,得到超连续谱的带宽越宽,实验与数值模拟结果一致.另外,要想获得平坦的宽带超连续谱,必须选择合适的光纤长度.     

飞秒光镊技术及其发展

激光捕获技术是利用光辐射力来捕捉、移动和操纵微粒的先进技术。飞秒光镊在实现粒子微纳操纵的同时还伴随着非线性现象的发生。阐述了飞秒光镊的模型和原理以及系统的各种结构形式,包括单光束梯度力光阱、贝塞耳光阱、双光束光纤光阱和冲击波光阱几种形式,并分析了每种形式的特点。     

基于双波长相干超短脉冲光源的超连续谱产生

提出在1550 nm波段采用双波长相干超短脉冲光源抽运高非线性光纤获得超连续谱。双波长脉冲抽运能够加强光纤中的四波混频和交叉相位调制过程,从而在相同抽运光功率下,可获得宽度远大于单波长脉冲抽运的超连续谱。利用基于脉冲切割器和Mamyshev再生器的25 GHz双波长相干超短脉冲光源,在入纤功率为24.1 d Bm时,获得了130 nm宽度的超连续谱,并进一步证实了所获超连续谱谱线之间的相干性。     

面向实用化的片上光阱传感单元

提出并实现了一种片式光阱传感单元.利用基片上的V型槽保证双光纤光阱的对准,利用压电元件振动使微球与基片脱离,实现了空气环境中对10μm微球的捕获,捕获后的微球位置稳定性达到0.12μm.在此基础上,设计制作了可预置少量微球的封闭微型腔,实现了光阱传感结构的微型化和集成化,解决了光阱中单微球的高效可重复起振难题,为实现实用化的光阱传感器奠定了基础.     

基于视觉原理和流体力学法的全息光镊三维光阱刚度测量

为了检测全息光镊的捕获能力,将基于视觉原理的光学测量系统用于全息光镊三维光阱刚度的测量.光学系统采用对称分布的双光源照射样品池,形成两个像点,通过跟踪像点位移获取被全息光镊捕获微球的三维位移信息.结合流体力学法测出本光学系统中单、双光阱的三维光阱刚度.实验结果表明:单光阱中,沿X、Y水平方向光阱刚度相差不大,而轴向光阱刚度落在水平方向的1/3~1/4范围;双光阱中,离光轴(即衍射中心)近的光阱其三维刚度要比距离光轴稍远的光阱要大,说明越靠近衍射中心,光的衍射效率越高,产生的光阱捕获能力也越强.     

低频响及低采样频率下用布朗运动分析法测量光阱刚度

布朗运动法测量光阱刚度因其简便易行而成为光镊系统参数测量的一种常用的方法，但是由于在低频响或低采样频率时测量结果误差很大，所以主要用在频响及采样频率较高的情况下.将在功率谱分析及实验的基础上，讨论如何在低频响及低采样频率下，用布朗运动法测量光阱刚度. 关键词： 布朗运动 光阱刚度 光镊 功率谱     

Investigation on the radius of a hemispherical microlens of an optical fiber end for three-dimensional trapping

We studied the optical forces acting on a sphere in order to corroborate the three-dimensional optical trapping by the laser beams from optical fiber ends inserted at an angle. From these investigations, we verified that there was only one stable point of equilibrium located below the beam-crossing point. Furthermore, we could reveal that employing microlenses in a dual-beam trap enhanced the trapping stability and increased the trapping volume.     

Single laser beam fiber optic trap

Optical forces acting on a sphere were experimentally analyzed to investigate the single-beam fiber optic trap using a cleaved optical fiber or a lensed optical fiber. A stable optical trap could be created at the point where the x-directed (horizontal) optical forces were precisely balanced, and the vector sum of axial and transverse forces acting on a sphere gave a restoring force directed back to the stable point. As compared with other embodiments, such as a single-beam gradient trap (optical tweezers) and dual-beam fiber optical traps, this single-beam fiber optic trapping was most economical, much simpler to operate, and required relatively low optical power to capture an object. Furthermore, a lensed optical fiber could easily trap and manipulate a micro object in comparison with a cleaved optical fiber because of the strong transverse optical confinement.     

小型化可调光镊设计

光镊利用强会聚激光对微粒产生的梯度力来捕获微粒,可以进行无损、远程操控,同时具有皮牛精度的测力特性,已经成为物理学、生命科学和胶体化学等研究领域中不可缺少的研究工具。光镊效应可以表现微小的光子动量和角动量,是物理学的重要教学工具。本文根据高斯光束传播和变换规律,设计具有稳定捕获性能的最小化光镊,并给出了典型参数。光镊系统由捕获激光、光束耦合系统、倒置生物显微镜和大数值孔径物镜组成,成像系统由物镜、摄影目镜和CCD相机组成。本光镊系统具有紧凑特性,同时通过保持物镜后瞳充满度来实现稳定捕获。在该最小光镊系统上,可以根据用户需求增加光镊阱位操控系统、刚度调节系统和其他辅助设备以满足不同操控要求,可以很好地满足科研和教学需求。     

液晶空间光调制器产生可调三光学势阱

提出了产生三光学势阱的新方案, 在该方案中用液晶空间光调制器制作相位型闪耀光栅, 单色相干光照明, 产生按等边三角形分布的三个光学势阱, 三个光阱光强大小分布相同, 调节空间光调制器的相位分布, 可以改变光阱的相对位置, 实现三光阱到单个光阱、两光阱合并为一个光阱等演变及其反向演变, 调节过程简单、方便. 根据现有空间光调制器性能和尺寸, 模拟设计光栅, 计算三光阱的光强分布和调控过程中光强的变化, 结果表明: 用一般功率的激光照明, 能够得到具有较大峰值光强和较高光强梯度的可调三光阱, 在原子和分子光学实验研究中有多种重要的应用. 关键词： 原子和分子光学 可调三光学势阱 空间光调制器     

Optical Force on a Micro-Sphere by the Laser Beam from a Lensed Optical Fiber Inserted at an Angle

Optical forces acting on a micro-sphere were studied to corroborate the single-beam fiber optic trap. Transverse optical force acted to pull the micro-sphere back to the beam axis, but axial force always acted in the direction away from the optical fiber end because the laser beam from optical fiber could not be strongly focused. A stable two-dimensional optical trapping could be obtained by the weakly focused laser beam from the fiber end inserted at an angle.     

基于自回归模型的光阱中粒子运动模拟

通过分析光阱中颗粒位移信号特性, 建立描述粒子受限布朗运动过程的自回归模型, 进而提出了一种基于自回归模型的光阱中颗粒运动信号模拟的新方法. 对半径为1 μm的粒子处于光阱刚度分别为10, 20, 50 pN/μm 光阱时的位移信号进行了模拟, 得到的模拟位移信号的自相关函数与理论值相一致. 为了进一步阐明自回归模型的有效性, 在相同光阱参数下, 分别采用自回归模型与蒙特卡罗方法模拟光阱中微粒的位移信号, 采用功率谱法分别对两种模拟方法所得的微粒位移标定光阱刚度, 结果表明自回归模型方法能够取得和蒙特卡洛法相同的精度. 因此, 本文为分析光阱中粒子的随机运动提出了一种新的模拟方法, 可以用来对光阱中的噪声及特性进行分析. 关键词： 光阱 布朗运动 信号模拟 自回归模型     

Monte-Carlo simulation of optical trap stiffness measurement

The high precision calibration of optical trap stiffness is the foundation of the weak force measurement in an optical tweezers system. And the accuracy of the trap stiffness measurement is limited by the bandwidth of the acquisition system. In this article, such an influence is analyzed and discussed. The stiffness measuring process using an acquisition system with a finite acquisition time is numerically simulated by using Monte-Carlo method. Then the simulated results are analyzed by thermal motion analysis method to deduce the trap stiffness for different trapping system and for measuring systems with different acquisition time. As a comparison the power spectrum analysis method is used to study the thermal motion of the bead and to compute the trap stiffness for the same acquisition system, from which it is concluded that the bandwidth of the acquisition system is determined by its acquisition time, not the sampling frequency. The influence of the finite acquisition time or the limited bandwidth on the trap stiffness measurement is discussed. The numerical simulation shows that the measured position, which is here the average position within the acquisition time, shifts to the trap center due to the trapping force, which gives an alternative interpretation for the deviation of the measured stiffness from the true trap stiffness.     

Experimental Analysis of Optical Trapping System Using Tapered Hemispherically Lensed Optical Fiber

We describe the operation and performance of an optical fiber trap realized using a tapered hemispherically lensed optical fiber. Axial and transverse trapping forces exerted on a microsphere are experimentally analyzed to corroborate the optical trapping using an optical fiber. Experimental results are as follows. (i) Transverse force F_tr acting on a sphere is a restoring force that acts to pull the microsphere back to the center of trap. (ii) Axial force F_ax always acts to push a sphere in the direction of the beam away from the trapping fiber end. (iii) Vector sum of F_tr and F_ax acting on a sphere gives a restoring force directed back to the stable point. (iv) Transverse force F_tr plays a significant role in trapping a micro-sized object by means of an optical fiber.This paper was originally presented at the 5th International Conference on NEAR FIELD OPTICS and RELATED TECHNOLOGIES (NFO-5), which was held on December 6–10, 1998 at Coganoi Bay Hotel, Shirahama, Japan, in cooperation with the Japan Society of Applied Physics and Mombusho Grant-in Aid for Scientific Research on Priority Areas “Near-field Nano-optics” Project, sponsored by Japan Society for the Promotion of Science.