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In the calibration of the optical trap stiffness, it is found that there appears an attenuating oscillation as an oscillatory disturbance added to the trapped bead movement, when the scanner is driven by a triangular wave input.An equivalent oscillator model is put forward to explain the mechanism of the oscillatory disturbance. Both the measurements and calculations show that the attenuating oscillation comes from the oscillation of the scanner and the triangular wave drive causes this additional oscillation of the scanner. Furthermore, the analysis indicates that the oscillatory disturbance will become stronger, when the stiffness of the trap increases or the natural frequency of the scanner decreases. We adopt another driving way, i.e. a sinusoidal wave input is used instead of the triangular wave input. Our experiment has verified that in this case the oscillatory disturbance is eliminated completely. 相似文献
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Membrane tethers are extracted from breast cancer cells using a force generated by an optical trap. It is experimentally obtained that the radius of tether is about 0.1μm and the static tether force is about 8.5 pN. Calculations based on the experimental measurements give a bending modulus for the tether of 1.35 x 10^-19 N.m and a surface membrane tension of 6.76 x 10^-6 N/m in the breast cancer cell. The treatment with cytochalasin D results in the decreasing bending modulus and decreasing apparent surface tension. When the membrane protein caveolin is over-expressed, similar cases occur in bending modulus and apparent surface tension. In addition, the viscous resistance coefficient of the membrane is calculated to be 1.15pN.s/μm according to the dynamic tether forces obtained under different pulling velocities. 相似文献
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In this letter,we propose a method for the numerical calculations of the femtosecond laser pulse passed through a subwavelength aperture.The time-dependent laser pulse is decomposed into a series of monochromatic simple harmoniv waves.For the light field of the harmonic wave with a single frequency,the numerical calculation is made based on the solution of the Green's integral equation set of the electro-magnetic waves.Such numerical solution is iterated for all the waves with different frequencies,and all thenumerical solutions are transformed into the light fields in the time domain by inverse Fourier transform.The light intensity distributions transmitted the subwavelength aperture are calculated and the resultsshow the propagation of the light field is along the direction of the medium interface. 相似文献
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遮挡情况下目标跟踪算法综述 总被引:1,自引:0,他引:1
目标跟踪是机器视觉的核心问题之一。由于被跟踪目标本身特征的多样性,所处环境的复杂性,使得目标跟踪成为一个极富挑战性的课题。特别是跟踪过程中出现的遮挡问题,一直是目标跟踪中的难点。如何有效处理遮挡,尤其是严重遮挡和全部遮挡,成为限制目标跟踪算法鲁棒性的关键因素。本文对国内外现有的遮挡情况下的目标跟踪算法进行了总结和分类,阐述了各种算法的基本思想,简要介绍了其代表性的算法,指出了算法的适用场合,分析了算法的优缺点。文章最后对遮挡情况下的目标跟踪做了必要的讨论。 相似文献
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图像增强是图像处理最有吸引力的领域。在嵌入式视频图像处理系统中,图像增强后的效果和图像增强的实时性是决定算法优劣的两个关键问题。而恰当的图像增强算法和高效硬件平台的合理搭建是解决问题的有效途径。在研究多种实时图像增强算法基础上,本文在matlab平台上对三种典型的增强算法进行算法仿真,即直方图均衡增强算法、基于照度-反射模型的同态滤波增强算法、基于小波变换增强算法。并对仿真结果进行分析,比较各算法的优缺点,指出了其最佳的应用场景和最高效的硬件实现平台,以期从中总结出一套行之有效的实时图像增强算法的应用指导规则。 相似文献
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经典的UNet网络可用于预测全分辨率空间域的密集位移场,在医学图像配准中取得了巨大成功。但对大变形的三维图像配准,还存在运行时间长、无法有效保持拓扑结构、空间特征易丢失等缺点。为此,提出一种基于交叉注意的大内核多尺度可变形医学图像配准网络(large kernel multi-scale deformable medical image registration network based on cross-attention,LK-CAUNet)。在经典UNet模型基础上,通过引入交叉注意力模块,实现高效、多层次的语义特征融合;配备大内核非对称并行卷积,使其具有多尺度特征和对复杂结构的学习能力;通过加入平方和缩放模块,实现拓扑守恒和变换可逆。基于脑部MRI数据集,将LK-CAUNet与18种经典图像配准模型进行了比较,结果表明,LK-CAUNet的配准性能较其他模型有明显提升,其Dice得分较TransMorph配准方法提高了8%,而参数量仅为TransMorph的1/5。 相似文献
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We propose a mathematical model to analyse the membrane tether formation process on a cell surface with reservoir. Based on the experimental results, the membrane reservoir density of breast cancer cell was obtained, ρ=8.02. The membrane surface viscosity between membrane and environment η is 0.021(pN.s/μm^3), and the static force F0=5.71pN. 相似文献
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