共查询到16条相似文献,搜索用时 109 毫秒
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光镊利用光学梯度力捕获和操控微小粒子,已经成为深入研究生物分子间相互作用等微观机制的独特技术.光镊光束操控系统一般由扩束输入镜、扩束输出镜、调焦透镜、耦合透镜和压电转镜等光学元器件组成,以保证物镜后瞳充满的前提下实现光镊阱位操控.光镊阱位的三维精确操控是实现光镊位钳和力钳模式的基本条件.本文根据矩阵光学,对基于无穷远校正显微镜的光镊操控光路进行计算,分析扩束输入镜、调焦透镜和物镜轴向位置调整,以及压电转镜、调焦透镜和耦合透镜安装位置误差对光镊径向阱位操控精度的影响,得到了物镜高度调整基本不会影响光镊径向位置操控,压电转镜和调焦透镜的安装位置误差对光镊径向阱位操控精度影响最大等结论,提出了能够实现径向阱位精确操控的轴向阱位动态操控范围,为光镊设计和操控提供理论和实验指导. 相似文献
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光镊是研究单分子生物物理特性的独特工具, 因而光镊设备的研发是一个极为重要的课题. 本文根据矩阵光学, 对基于有限远共轭显微镜的光镊操控光路进行计算, 得出了阱位径向操控和轴向操控方程, 并分析了光束调控系统、 共焦系统后置透镜和耦合透镜安装位置误差及物镜轴向位置调整对光镊阱位径向及轴向操控精度的影响. 计算结果显示, 当物镜初级像面和耦合透镜像方焦面完全重合, 光束调控系统和耦合透镜的距离误差对阱位径向和轴向操控精度没有影响. 光镊系统元器件定位不准时, 基于无限远共轭显微镜光镊的阱位径向操控误差和轴向操控误差都小于基于有限远共轭显微镜光镊的阱位径向操控误差和轴向操控误差. 当光镊耦合透镜定位误差控制在小于10 mm时, 基于有限远共轭显微镜光镊的径向和轴向操控误差分别小于5.9%和11.4%, 有限远共轭显微镜仍然存在改造为光镊的价值.本文理论为基于有限远共轭显微镜的光镊设计、改造和操控提供理论和实验指导.
关键词:
光镊
光学设计
矩阵
误差 相似文献
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传统的光镊技术使用单个物镜同时进行光学捕获与显微成像,使得捕获与成像区域被限制在物镜焦平面附近,无法同时观察到沿光轴方向(即Z向)捕获的多个微粒.本文提出一种轴平面(XZ平面)GerchbergSaxton迭代算法来产生沿轴向分布的多光阱阵列,将轴平面成像技术与光镊结合,实现了沿轴向对二氧化硅微球的多光阱同时捕获与实时观测.通过视频分析法测量了多个二氧化硅微球在轴向光镊阵列中的布朗运动,并标定了光阱刚度.本文提出的轴向多光阱微粒捕获与实时观测技术为光学微操纵提供了一个新的观测视角和操纵方法,为生物医学、物理学等相关领域研究提供了一种新的技术手段. 相似文献
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碳酸钙微粒光致旋转的实验和理论研究 总被引:3,自引:0,他引:3
理论分析了由于光束轨道角动量和自旋角动量传递以及微粒的特殊形状导致微粒旋转的机理.实验建立了单光束激光光镊装置,不仅可以捕获并移动直径为微米量级的微小粒子,而且利用圆偏振光与微粒之间角动量的传递,实现了对具有双折射特性的碳酸钙微粒的光致旋转.实验中发现微粒的旋转不仅取决于光束的偏振态,还与微粒本身的形状有关,解释了实验中观察到的几种旋转现象.碳酸钙微粒旋转的最高转速达到12转/秒,转速与激光功率成正比. 相似文献
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Optical tweezers have been successfully used in the study of colloid science. In most applications people are concerned with the behaviour of a single particle held in the optical tweezers. Recently, the ability of the optical tweezers to simultaneously hold two particles has been used to determine the stability ratio of colloidal dispersion. This new development stimulates the efforts to explore the characteristics of a two-particle system in the optical tweezers.An infinite spherical potential well has been used to estimate the collision frequency for two particles in the optical trap based on a Monte Carlo simulation. In this article, a more reasonable harmonic potential, commonly accepted for the optical tweezers, is adopted in a Monte Carlo simulation of the collision frequency. The effect of hydrodynamic interaction of particles in the trap is also considered. The simulation results based on this improved model show quantitatively that the collision frequency drops down sharply at first and then decreases slowly as the distance between the two particles increases. The simulation also shows how the collision frequency is related to the stiffness of the optical tweezers. 相似文献
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Optical tweezers with a low numerical aperture microscope objective is used to manipulate the microspheres at the water-air interface. In this letter, we determine the optimal optical trap for the lateral manipulation of microspheres at a water-air interface. The experimental results show that the trapping force is influenced by the expansion of the trapping beam at the back aperture of the objective. The optimal filling ratio of 0.65 is suggested for lateral optical manipulation at the water-air interface. The lateral trapping forces at the water-air interface are theoretically investigated with the ray-optics model. The numerical results show that the lateral trapping forces can be changed by shrinking the diameter of the trapping laser beam. The numerical results are in accordance with the experimental results. 相似文献
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Xiumin Gao Zhuo Li Jian Wang Lingling Sun Songlin Zhuang 《Optics and Lasers in Engineering》2010,48(7-8):766-773
Gradient force plays an important role in optical tweezers technique. In this paper, the tunable gradient force in focal plane of the hyperbolic-cosine–Gaussian (ChG) beam is investigated numerically. The ChG beam contains one spiral vortex and one non-spiral vortex. Simulation results show that the gradient force distribution can be altered considerably by decentered parameters of ChG beam, topological number of the spiral vortex, and vortex parameter of the non-spiral vortex. Many novel gradient force patterns can occur, which means corresponding optical traps may come into being, including ring optical trap, multiple-point trap pattern, line optical trap, rectangle trap pattern, and rhombus trap pattern. In addition, force pattern evolution principle may also differ significantly. 相似文献
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The focusing properties of a concentric piecewise cylindrical vector beam is investigated theoretically in this paper. The beam consists of three portions with different and changeable phase retardation and polarization. Numerical simulations show that the evolution of the focal shape is very considerable by changing the radius and polarization rotation angle of each portion of the vector beam. And some interesting focal spots may occur, such as two- or three-peak focus, dark hollow focus, ring focus, and two-ring-peak focus. Corresponding gradient force patterns are also computed, and novel trap patterns, including cup shell shape trap with one trap at its each side along axis, rectangle shell shape trap with one trap at its each side, dumbbell optical trap, spherical shell optical trap, may occur, which shows that the concentric piecewise cylindrical vector beam can be used to construct controllable optical tweezers. 相似文献
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Optical forces acting on a sphere were experimentally analyzed to investigate the single-beam fiber optic trap using a cleaved optical fiber or a lensed optical fiber. A stable optical trap could be created at the point where the x-directed (horizontal) optical forces were precisely balanced, and the vector sum of axial and transverse forces acting on a sphere gave a restoring force directed back to the stable point. As compared with other embodiments, such as a single-beam gradient trap (optical tweezers) and dual-beam fiber optical traps, this single-beam fiber optic trapping was most economical, much simpler to operate, and required relatively low optical power to capture an object. Furthermore, a lensed optical fiber could easily trap and manipulate a micro object in comparison with a cleaved optical fiber because of the strong transverse optical confinement. 相似文献