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The overall history and recent advances in surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) technology is reviewed herein. Fundamentals of SELDI-TOF analysis are presented while drawing comparisons with other laser-based mass spectrometry techniques. The application of SELDI-TOF-MS to functional genomics and biomarker discovery is discussed and exemplified by elucidating a biomarker candidate for prostatic carcinoma. Finally, a short discussion regarding future SELDI requirements and developments is supplied.  相似文献
2.
The kinetics of ligand substitution reactions of [Ru(H2dtpa) (H2O)] (2) (H2dtpa=diprotonated diethylenetriaminepentaacetic acid) were studied as a function of ligand (L) concentration, pH (2.5–8.0) and temperature (30–45 °C) at 0.2 M ionic strength. The equilibrium constants for the formation of mixed ligand complex [RuIII(dtpa) (L)] (L=2-mercaptopyrimidine, cysteine) and the distribution of various species in solution in the pH range of 2.5–8.0 were computed from potentiometric results. [Ru(H2dtpa) (H2O)] ( H2dtpa= ) , pH (2,5–8,0) (30–45°C) 0,2 M. [RuIII(dtpa) (L)] ( L=2-, ) pH=2,5–8,0.  相似文献
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