基于生物质谱技术的磷酰化修饰策略在多肽测序中的应用

该文建立了一种利用磷酰化修饰结合电喷雾质谱(ESI-Q-TOF)测定多肽氨基酸序列的有效方法。利用Atherton-Todd反应,以二丙基亚磷酰酯(DPP)为磷酰化试剂,应用生物质谱技术,对磷酰化修饰后的5种模型肽的磷酰化反应情况进行了系统研究,考察了磷酰化肽的二级质谱特征,并与未经磷酰化反应的肽的二级质谱特征对比。结果表明,经过磷酰化修饰后,肽的二级质谱中的a1离子信号强度明显增加,可以准确鉴定其N端氨基酸;b系列离子信息完整,信号强度增强,使得多肽C ID测序的谱图简单、清晰,有利于肽的氨基酸序列的测定;赖氨酸(K,128.10 u)和谷氨酰胺(Q,128.13 u)两种氨基酸质荷比相近,由于二者磷酰化修饰后的差异性,使其得到准确区分。经过5种已知氨基酸序列的模型肽的磷酰化后结合质谱技术进行氨基酸序列测定验证,结果表明该方法简单、快速、准确,提高了利用质谱技术进行多肽测序的准确度和灵敏度,可为蛋白质组学研究提供有效的技术手段。     

Cosmic ray test for a T0 detector

The endcap of time-of-flight (ETOF) detector in BES (Beijing Spectrometer) III is planned to be upgraded by using multi-gap resistive plate chambers (MRPCs) and the designed time resolution of the MRPCs is around 50 ps. Thus a time-zero (T0) detector needs to be built to offer a high quality reference time for the MRPCs beam test. So a T0 detector is built using plastic scintillator tiles (BC420) to couple with four fast phototubes (PMTs, Hamamatsu H6533). The timing properties of the detector is studied by using a cosmic ray test and factors related to the time resolution, such as plastic scintillator size, readout mode and angle effects, are discussed. T0 detector timing resolutions of ～41-62 ps are achieved, which means that the T0 detector can be used in the MRPC beam test.     

结构物在水下运行过程中的自振特性及动力响应研究

本文运用流体力学、结构动力学及变分原理推导了结构－流体耦合系统的动力学方程，并对水下结构物在运行时的自振特征及动力响应给出了数值计算分析。     

非线性方程的保辛近似算法

保守体系的微分方程可用Hamilton体系的方法描述,其特点是保辛。两个辛矩阵之和不能保辛,两个辛矩阵的乘积仍是辛矩阵。最常用的小参数摄动法用的是加法,因此对辛矩阵不能保辛。从保辛的角度,要用正则变换。本文针对非线性微分方程,运用自变量坐标变换,对原系统进行变换。由此推导出变换后系统的变分原理。引入Hamilton对偶变量,通过数学变换,得到变系数非线性方程。针对该方程,本文提出了保辛摄动算法。通过数值算例,对不同步长下,保辛摄动法、多尺度摄动法、龙格库塔法和精确解的结果做了比较。数值例题表明,对于非线性方程,本文提出的保辛摄动算法有良好的精度。在步长增大的情况下,保辛摄动保持了良好的稳定性。     

用连续记录的方法测量高Tc超导材料的转变温度

本文介绍了用连续测量和记录的方法，准确测量高Ｔｃ超导材料的转变温度和实验装置。     

Experimental validation of domain-level gradient-based and hierarchical PCE-based routing and control in heterogeneous optical networks

A domain-level gradient-based routing （DLR） algorithm for heterogeneous optical networks with syn- chronous digital hierarchy and optical transport network domains is proposed and experimentally vali- dated. This algorithm classifies domains into groups with incremental levels on the basis of domain-level partitioning, and guides paths level by level along a gradient on the basis of interdomain routing tree evolution. The proposed algorithm is implemented in the hierarchical path computation element-based control architecture for connection provisioning. Testbeds with commercial and emulated nodes are es- tablished to verify the feasibility and performance of the algorithm. Experimental and emulation results show that DLR effectively performs in terms of network blocking probability, real time characteristics, and scalability.     

不可压缩材料分析的界带有限元

针对完全不可压缩材料系统(ν=0.5),提出流函数的概念,给出相应的变分原理,并进行数值分析．流函数的引入涉及到高阶微分,为此提出界带有限单元．该单元基于界带理论,能够很好地解决具有高阶微商的变分原理所带来的高阶近似问题．     

A position-sensitive detector with lithium glass and MaPMT

A position-sensitive detector is designed for neutron detection. It uses a single continuous screen of a self-made lithium glass scintillator, rather than discrete crystal implementations, coupling with a multi-anode PMT (MaPMT). The scintillator is fast and efficient; with a decay time of 34 ns and thermal neutron detection efficiency of around 95.8% for the 3 mm thick screen, and its light yield is around 5670 photons per neutron and 3768 photons per MeV γ rays deposition. The spatial resolution is around 1.6 mm (FWHM) with the energy resolution around 34.7% by using α (5.2 MeV) rays test.     

奇异Hamilton系统矩阵的精细积分法

常规位移有限元的结构振动方程是n个二阶常微分方程组.采用一般交分原理推导,将结构振动问题引入Hamiltoil体系,将得到2n个一阶常微分方程组.精细积分法宜于处理一阶方程,应用于线性定常结构动力问题求解,可以得到在数值上逼近精确解的结果.对于非齐次动力方程,当结构具有刚体位移时,系统矩阵将出现奇异.本文借鉴全元选大元高斯-约当法求解线性方程组的经验,提出全元选大元法求奇异矩阵零本征解的方法,该方法可以简便快速地寻求奇异矩阵零本征值对应的子空间.利用Hamiltoil体系已有研究成果及Hamilton系统的共轭辛正交归一关系,迅速将零本征值对应的子空间分离出来,通过投影排除奇异部分,然后用精细积分法求得问题的解.数值算例表明,该方法对Hamilton系统奇异问题,处理方便,计算量小,易于实现,同时保持了精细算法的优点.