抗独特型抗体在小分子物质免疫检测中的应用

抗独特型抗体是机体针对抗体可变区所产生的特异性抗体.基于其独有的性质,它在小分子物质免疫检测中可以替代有毒标准品建立无毒化检测技术; 也可以用来建立新的免疫检测模式,特别是非竞争模式,从而提高传统免疫检测技术的特性;还能够在多残留检测中发挥优势.抗独特型抗体制备困难是制约其应用的瓶颈,但噬菌体展示库技术为解决此问题提供了一条有效途径.     

Dynamic behavior of non-uniform granular gases system with the fractal characteristic in one dimension

A one-dimensional dynamic model of polydisperse granular mixture with a power-law size distribution is presented, in which the particles are subject to inelastic mutual collisions and driven by Gaussian white noise. The particle size distribution of the mixture has the fractal characteristic, and a fractal dimension D as a measurement of the inhomogeneity of the particle size distribution is introduced. We define the global granular temperature and the kinetic pressure of the mixture, and obtain their expressions. By molecular dynamics simulations, we have mainly investigated how the inhomogeneity of the particle size distribution and the inelasticity of collisions influence the steady-state dynamic properties of the system, focusing on the global granular temperature, kinetic pressure, velocity distribution and distribution of interparticle spacing. Some novel results are found that, with the increase of the fractal dimension D, the global granular temperature and the kinetic pressure decrease, the velocity distribution deviates more obviously from the Gaussian one and the particles cluster more pronouncedly at the same value of the restitution coefficient e (0<e<1). On the other hand, as the restitution coefficient e decreases, the dynamic behavior has the similar evolution as above at the fixed fractal dimension D. The dynamic behavior changing with e and D is, respectively, presented.     

SECRAL在18GHz频率下产生强流高电荷态离子束的初步结果

A Superconducting ECR ion source with Advanced design in Lanzhou (SECRAL) was successfully built to produce intense beams of highly charged ions for Heavy Ion Research Facility in Lanzhou (HIRFL).The ion source has been optimized to be operated at 28GHz for its maximum performance.The superconducting magnet confinement configuration of the ion source consists of three axial solenoid coils and six sextupole coils with a cold iron structure as field booster and clamping.For 28GHz operation,the magnet assembly can produce peak mirror fields on axis 3.6T at injection,2.2T at extraction and a radial sextupole field of 2.0T at plasma chamber wall.A unique feature of SECRAL is that the three axial solenoid coils are located inside of the sextupole bore in order to reduce the interaction forces between the sextupole coils and the solenoid coils. During the ongoing commissioning phase at 18GHz with a stainless steel chamber,tests with various gases and some metals have been conducted with microwave power less than 3.2kW and it turned out the performance is very promising.Some record ion beam intensities have been produced,for instance,810eμA of O~(7 ),505eμA of Xe~(20 ),306eμA of Xe~(27 ),21eμA of Xe~(34 ),2.4eμA of Xe~(38 ) and so on.To reach better results for highly charged ion beams,further modifications such as an aluminium chamber with better cooling,higher microwave power and a movable extraction system will be done,and also emittance measurements are being prepared.     

中能重离子微束辐照装置的真空窗

为了辐照生物活细胞,束流须经由真空隔离窗引入大气环境中.在真空隔离窗的设计中,对结构、材料种类及其厚度的选择既要尽可能地减小束斑展宽,又要兼顾安全性.应用有限元分析软件对不同结构、不同材料的真空窗进行受力分析,模拟真空窗的形变和等效应力,运用强度理论考察候选真空窗的安全性;并运用SRIM程序模拟离子的小角散射、横向射程,计算入射离子的能量、真空窗材料及其厚度、空气层对束斑展宽的影响;最后提出真空窗适宜的结构、材料及厚度范围.     

Molecular mechanism of Peptide-induced pores in membranes

We suggest a physical mechanism by which antimicrobial peptides spontaneously induce stable pores in membranes. Peptide binding to a lipid bilayer causes an internal stress, or internal membrane tension, that can be sufficiently strong to create pores. Like detergents, peptides have a high affinity for the rim of the pore. Binding to the rims reduces the line tension and decreases the number of peptides causing the internal membrane tension. Consequently, the pore radius is stable. The pore formation resembles a phase transition.     

CSR放射性次级束流线

兴建中的放射性次级束流线,是兰州重离子冷却储存环中连接主环和实验环的束运线的主要部分.描述它的结构、离子光学,以及二、三阶像差校正.     

甲氧基有机磷杀虫剂广谱特异性抗体的制备

以通用结构O,O-二甲基硫代磷酸酯为目标检测基团,制备针对甲氧基有机磷杀虫剂的广谱特异性抗体。利用O,O-二甲基硫代磷酸钠和氯乙酸合成半抗原S-羧甲基-O,O-二甲基二硫代磷酸酯(CMP),通过混合酸酐法(MA)和活性酯法(AE)分别与牛血清蛋白(BSA)和卵清蛋白(OVA)偶联。CMP-MA-BSA、CMP-AE-BSA作为免疫原均获得了免疫应答。其中,CMP-AE-BSA所获得的抗血清效价最高,为256000。研究了有机溶剂种类及含量、pH因素对ELISA曲线的影响,确定CMP酶联免疫分析方法(ELISA)的最佳工作条件,CMP的最低检测浓度为0.076μg/L,IC50为93.97μg/L。以14种常见有机磷杀虫剂为对象,检测抗体对其交叉反应,测定结果表明:抗体对马拉硫磷、稻丰散、乐果、亚胺硫磷、倍硫磷、甲基嘧啶磷、甲基对硫磷、杀螟硫磷及杀扑磷等均有识别作用。IC50分别为69.92、136.90、230.39、416.84、508.57、510.38、607.21、835.30和850.21μg/L。该技术可用于多种甲氧基有机磷杀虫剂的快速定性或半定量检测。     

近红外光谱指纹分析在羊肉产地溯源中的应用

为寻求低廉、快速有效地签别羊肉产地来源的方法,对来自内蒙古自治区锡林郭勒盟、呼伦贝尔市和阿拉善盟三个牧区,及重庆市和山东省菏泽市两个农区共99份羊肉样品进行近红外光谱扣描,利用主成分分析结合线性判别分析(PCA+LDA),以及偏最小二乘判别分析法(PLS-DA)对光谱数据进行了分析,建立了羊肉产地来源的定性判别模型.结...     

SrAl2O4∶Eu2+，Dy3+长余辉材料发光性能与温度依赖研究

对SrAl₂O₄∶Eu²⁺,Dy³⁺长余辉材料在100~500 K温度之间的发光性能进行研究。实验结果表明,材料的荧光及余辉强度在特定温度区间内呈线性变化,在热释峰所在温度范围具有较好的发光性能。其变化规律表明SrAl₂O₄∶Eu²⁺,Dy³⁺长余辉材料内部陷阱中电子的释放包括瞬时释放和延时释放两种类型,其中电子瞬时释放进而跃迁发光是荧光的组成部分,延时释放产生的跃迁则导致余辉发光。陷阱和电子的复合与陷阱中电子释放过程均随温度升高而增强,但温度过高时会发生热猝灭。材料荧光强度与余辉强度在特定温度区间内随温度呈线性变化关系表明其可以作为一种光纤温度传感材料。     

以活性炭为基材负载季鏻盐多孔碳材料的制备与表征

以市售普通活性炭为碳源,先对其进行强碱活化处理制得多孔碳材料,再经硝酸氧化后,通过水热溶剂法将季鏻阳离子负载于多孔碳上,得到功能化的多孔碳材料。通过比表面积(BET)、热重分析(TGA)、X射线衍射(XRD)、高分辨透射电镜(HRTEM)以及ζ电位对其形貌和结构进行分析,并对其抗菌活性进行表征。结果表明,该方法成功合成了功能化多孔碳材料,该碳材料在水溶液中具有较好的分散性、稳定性和荷电性,并对大肠杆菌(E.coli)和金黄色葡萄球菌(S.aureus)具有良好的抗菌活性。