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乳胶微球固定化碱性磷酸酶的研究 总被引:3,自引:0,他引:3
本文系统地研究了生物素化碱性磷酸酶在微球载体上的固定化,确定了在胺化聚苯乙烯微球上固载的最适条件,并对所得固相酶的最适pH值,最适温度,米氏常数Km值等性能进行了测试,结果发现生物素化碱性磷酸酶固定于胺化微球后其最适pH值从9.5降低至9.3;酶的最适温度没有发生变化,但范围稍加宽;在50℃温度下处理90分钟,固相酶活化保持在40%,稳定性增强,固定化后酶的Km减小。 相似文献
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聚苯乙烯阴离子交换树脂固定α—淀粉酶的研究(Ⅱ) 总被引:3,自引:0,他引:3
用聚苯乙烯阴离子交换树脂作载体,采用吸附法将a一淀粉酶固定化,研究了固定化条件和固定化酶的性能。固定化条件为:酶浓度10mg/ml,固定化pH6.8,吸附时间16h,吸附温度6℃。固定化酶的pH稳定性、热稳定性、贮存稳定性都有一定的提高。最适作用温度及PH较自由酶为高,Km值为自由酶的1.6倍。同时也进行了固定化酶的操作稳定性研究。 相似文献
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本文用共沉淀法制备了平均直径为384纳米的α,ω-二羧基聚乙二醇磁性毫微粒,碱性蛋白酶通过吸附交联法被固定于磁性毫微粒,研究了制备研究中的吸附时间,给酶量,戊二醛浓度,pH和离子强度对磁性固定化酶活力及酶固定化率的影响。比较了碱性蛋白酶磁性固定化酶与自由酶的酶学性质,磁性固定化酶的最适温度没有改变,但热稳定性显著使高;磁性固定化酶的最适pH向酸性方向移动了1.0个pH单位。 相似文献
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以氨丙基多孔球为载体,戊二醛为交联剂制得固定化α-胰凝乳蛋白酶。其含酶量为3.6mg/g,米氏常数Km=1.2×10^-3mol/L,最适宜温度为35℃,pH值为7.0,对热、酸碱、甲醇以及尿素的稳定性有较大提高,Ca^2+激活效应明显。用该固定化酶连续拆分DL-苯丙氨酸制备L-苯丙氨酸时,拆分产率高于90%,产品纯度超过96%;连续使用2个月后仍能保持较高酶活力。 相似文献
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谷氨酸脱羧酶在新型羧甲基化交联烯丙基葡聚糖凝胶树脂上的 … 总被引:1,自引:0,他引:1
本文首次用合成的羧甲基化的以N,N’-甲叉双丙烯酰胺交联的烯丙基葡聚糖(简称CM-CADB)凝胶生化树脂为新型载体,研究了谷氨酸脱羧酸在CM-CADB树脂上的固定化与环境的信赖关系。确定了酶固定化最佳条件:缓冲体系为pH4.4,0.10mol/L磷酸氢二钠-0.05mol/L柠檬酸,载体为交联度35%,交换容量3.0meq/g的CM-CADB凝胶树脂,吸附平衡时间4h以上,酶用量为30.0mg/g 相似文献
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固定化根霉细胞催化拆分(R,S)-环氧丙醇丁酸酯的研究 总被引:3,自引:0,他引:3
用聚乙烯醇包埋、硫酸钠固定法对根霉( Rhizopussp.Bc0-09)菌体细胞进行了固定化,所得凝胶具有良好的机械性和稳定性. 对固定化细胞催化( R,S)-环氧丙醇丁酸酯水解反应的性能进行了研究. 对pH值的影响考察结果表明, 30 ℃下, 反应初速度在pH 值为5.5 时最大; 酶的立体选择性在pH值为5.5~6.0 时最高, 对映体比率( E值)为57. 对温度的影响考察结果表明,pH 值为5.5, 反应初速度在55 ℃时最大; 酶的立体选择性随温度升高而下降. 固定化细胞具有较好的操作稳定性. 相似文献
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报道了一种新的L-氨基酸氧化酶电极,这种酶电极系由氨气敏电极和以氨基化玻璃布为载体的酶膜所组成;研究了固定化条件对酶膜活性的影响以及底物浓度、温度和pH对电极响应特性的影响。该电极在6.0×10^-5~4.0×10^-3mol/L的底物浓度范围内呈良好的线性关系,检测下限为5.0×10^-5mol/L。在最宜条件下,酶电极具有良好的稳定性。 相似文献
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Uroš Grošelj Mojca Žorž Amalija Golobič Branko Stanovnik Jurij Svete 《Tetrahedron》2013,69(52):11092-11108
A new and simple synthesis of novel N-protected methyl 5-substituted-4-hydroxypyrrole-3-carboxylates, which exist in equilibrium with their 4-oxo tautomers, has been developed in two steps starting from N-protected α-amino acids. The key intermediates are enaminones, which can also be isolated, characterized, and used for the construction of other functionalized heterocycles, before they spontaneously decompose to pyrrole products. 4-Hydroxypyrroles are prone to partial aerial oxidation but can be efficiently alkylated or reduced to stable polysubstituted pyrrolidine derivatives. 相似文献
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The chemoselectivity in the intramolecular CH insertion of various diazosulfonamides has been experimentally studied. The results reveal that the aliphatic 1,4-, 1,5-, or 1,6-C(sp3)?H insertions of diazosulfonamides are not accessible, while the aromatic 1,5-C(sp2)?H insertion can be realized specifically by adjusting the diazo-adjacent group. In addition, the general chemoselectivities in the intramolecular CH insertions of diazosulfonyl compounds are summarized. Generally, diazosulfones undergo both aromatic 1,5-C(sp2)?H and aliphatic 1,5- and 1,6-C(sp3)?H insertions, while diazosulfonates undergo aliphatic 1,5- and 1,6-C(sp3)?H insertions. However, diazosulfonamides only undergo aromatic 1,5-C(sp2)?H insertion. 相似文献
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N-Heterocyclic carbene-palladacyclic complexes 3 were successfully achieved in a one-pot procedure under mild conditions. The structure of 3a was unambiguously confirmed by X-ray single crystal diffraction and it was an active catalyst in the Buchwald-Hartwig amination and α-arylation of ketones even at very low catalyst loadings (0.01?mol%). 相似文献
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Shashikant U. Dighe Surya K. Samanta Shivalinga Kolle Sanjay Batra 《Tetrahedron》2017,73(17):2455-2467
An efficient iodine-mediated oxidative Pictet-Spengler reaction in dimethyl sulphoxide (DMSO) using terminal alkynes as the 2-oxoaldehyde surrogate for the synthesis of aryl (9H-pyrido[3,4-b]indol-1-yl)methanones is described. The scope of the protocol includes the total synthesis of Fascaplysin, Eudistomins Y1 and Y2. The methodology is extended for preparing pyrrolo[1,2-a]-quinoxaline and indolo[1,5-a]quinoxaline derivatives. The utility of 1-aroyl-β-carbolines was demonstrated by performing palladium-catalyzed β-carboline directed ortho-C(sp2)-H functionalization of the phenyl ring with thiomethyl (SMe) group using DMSO as source and for accessing 4-aryl-canthin-6-ones. 相似文献
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In this Letter, we described a facile method for constructing fused bicyclic 1-arylpyrazol-5-one ring system. We employed various methylene-containing carboxylic acids as the substrates and proved that the pyrazolone ring closure requires activated methylene group in intermediate II. Accordingly, a series of structurally diversified, fused bicyclic 1-arylpyrazol-5-ones was prepared in moderate to high yields using the requisite substrates. 相似文献
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Synthesis of substituted pyrrolo[1,2-a]pyrazines and pyrazino[1,2-a]indoles from the Morita-Baylis-Hillman derivatives of acrylates via saponification followed by Curtius reaction is described. 相似文献
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用正丁胺作为碳源,采用射频辉光放电制备碳膜,选用激光染料R6G和聚乙二醇混合液作为蒸气源,采用单源热蒸发,在蒸发室与染料同时沉积得到混合膜,用拉曼光谱和红外光谱分析了碳膜的结构和键合方式,分析表明:碳膜中存在胺基团和氢原子.混合膜的荧光谱测量结果表明,认为正丁胺对染料荧光谱的影响是因为胺基和氢原子的存在. 相似文献
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Jona Mirnik Eva Pušavec Kirar Sebastijan Ričko Uroš Grošelj Amalija Golobič Franc Požgan Bogdan Štefane Jurij Svete 《Tetrahedron》2017,73(24):3329-3337
A series of 20 CuAIAC reactions between eight 4-acylamino substituted pyrazolidine-3-one-1-azomethine imines and four terminal ynones were performed using Cu0 as catalyst. The corresponding fluorescent cycloadducts were obtained in very high yields upon simple workup. Thus, Cu-metal turned out to be a better catalyst than CuI in terms of yield and ease of isolation. Availability of azomethine imines, mild reaction conditions, and simple workup enable a “click” access to libraries of densely substituted 2,3-dihydro-1H,5H-pyrazolo[1,2-a]pyrazol-1-ones. Reactivity of differently substituted dipoles was evaluated experimentally and by quantum chemical methods (DFT). 相似文献
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George E. Magoulas Thomas Garnelis Constantinos M. Athanassopoulos Dionissios Papaioannou George Mattheolabakis Konstantinos Avgoustakis Dimitra Hadjipavlou-Litina 《Tetrahedron》2012,68(35):7041-7049
(E)-4-(Fullerenopyrrolidin-1-yl)-3-methylbut-2-enoic acid and its corresponding succinimidyl ester, readily obtained through Prato-type modification of C60, were used for the selective N-acylation of polyamines. The thus obtained conjugates were evaluated for their antioxidative and anti-inflammatory activity and their cytotoxicity was determined. Members of this family of compounds showed interesting anti-lipid peroxidation, anti-lipoxygenase and anti-inflammatory activity and comparable cytocompatibility to spermidine. 相似文献