共查询到20条相似文献,搜索用时 93 毫秒
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荧光光谱法研究木犀草素、芹菜素或葛根素与牛血清白蛋白的相互作用机理 总被引:1,自引:0,他引:1
利用紫外可见光谱和荧光光谱法研究三种黄酮类药物木犀草素、芹菜素和葛根素与牛血清白蛋白(BSA)在不同温度下(292 K和311 K)的相互作用。结果表明:三种黄酮类药物与牛血清白蛋白形成复合物导致牛血清白蛋白荧光猝灭,试验数据采用Stern-Volmer拟合方程处理及双对数方程处理,进一步证明结合反应引起的荧光猝灭属于静态猝灭。采用位点结合模型公式、热力学公式和F rster非辐射能量转移理论计算了结合常数、结合位点数及作用力类型以及结合距离。 相似文献
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《分析试验室》2015,(5)
利用紫外光谱法和荧光光谱法研究了苋菜红的解离常数及其与牛血清白蛋白(BSA)的结合行为。应用荧光猝灭现象和Frster理论,考察了不同环境温度和酸度下苋菜红与牛血清白蛋白的结合常数和结合位点,并对其热力学常数进行了研究。苋菜红的解离常数pKa为10.43,与牛血清白蛋白间主要存在的是疏水作用和静电作用,二者间的结合距离为3.83 nm。结合过程中静态猝灭和非辐射能量转移是导致牛血清白蛋白荧光猝灭的原因。通过同步荧光光谱和荧光探针技术证明与苋菜红作用后的BSA分子二级结构发生了变化,苋菜红与其在亚结构域IIA(Site I)发生了结合。研究结果将为苋菜红在环境和生物体内的吸收、分布、代谢等行为的探索提供一定的理论基础。 相似文献
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The interactions between potassium perfluorooctanesulfonate (PFOS) and bovine serum albumin (BSA) were studied by fluorescence spectroscopy. The association constants between PFOS and BSA were obtained by fluorescence enhancing and fluorescence quenching respectively. Furthermore, fluorescence quenching was studied at different temperatures, and the binding constant was also determined by the method of fluorescence quenching. According to the thermodynamic parameters, the main binding force could be judged. The experimental results revealed that BSA and PFOS had strong interactions. The mechanism of quenching belonged to dynamic quenching and the main sort of binding force was hydrophobic force. IR-spectra proved the interaction changed the conformation of BSA. 相似文献
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四溴双酚-A与牛血清白蛋白相互作用的光谱学及电化学研究 总被引:1,自引:0,他引:1
采用荧光光谱、同步荧光光谱、三维光谱和循环伏安曲线法研究了溴化阻燃剂四溴双酚-A(TBBPA)与牛血清白蛋白(BSA)的相互作用。研究表明,TBBPA对BSA的内源荧光有显著的猝灭作用,根据不同温度下TBBPA对BSA的荧光猝灭作用及室温的循环伏安曲线,证实猝灭机理为静态猝灭。运用位点模型计算出结合常数KA和结合位点数n,说明TBBPA与BSA仅有1个结合位点。由ΔH0和ΔS0分别为-33.06 kJ/mol和-64.73 J/(mol.K),推断范德华力或氢键在二者结合过程中起主要作用。并通过同步荧光光谱和三维光谱研究了TBBPA对BSA构象的影响,结果表明,TBBPA分子的引入改变了BSA疏水腔内疏水微环境,从而导致BSA的构象发生变化。 相似文献
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Xu H Liu Q Wen Y 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2008,71(3):984-988
The interaction of nicotinamide (NA) and bovine serum albumin (BSA) was studied by fluorescence and absorption spectroscopy at different temperatures. The results revealed that NA caused the fluorescence quenching of BSA through a static quenching procedure. The binding constants K(A), and the number of binding sites n, corresponding thermodynamic parameters DeltaG, DeltaH, DeltaS between NA and BSA at different temperatures were calculated. The primary binding pattern between NA and BSA was interpreted as hydrophobic interaction. In addition, the effect of NA on the conformation of BSA was analyzed using synchronous fluorescence spectroscopy. The binding average distance, r between the donor (BSA) and acceptor (NA) was determined based on the F?rster's theory and it was found to be 3.1 nm. 相似文献
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Yu X Lu S Yang Y Li X Yi P 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2011,83(1):609-613
The fluorescence and ultraviolet spectroscopies were explored to study the interaction between N-confused porphyrins-edaravone diad (NCP-EDA) and bovine serum albumin (BSA) under simulative physiological condition at different temperatures. The experimental results show that the fluorescence quenching mechanism between NCP-EDA and BSA is a combined quenching (dynamic and static quenching). The binding constants, binding sites and the corresponding thermodynamic parameters (ΔG, ΔH, and ΔS) of the interaction system were calculated at different temperatures. According to F?rster non-radiation energy transfer theory, the binding distance between NCP-EDA and BSA was calculated to be 3.63 nm. In addition, the effect of NCP-EDA on the conformation of BSA was analyzed using synchronous fluorescence spectroscopy. 相似文献
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The interactions between bilirubin (BR) and bovine serum albumin (BSA) have been studied by fluorescence spectroscopy. The
association constant between BR and BSA was obtained by fluorescence enhancement titration. Furthermore, fluorescence quenching
was studied at different temperatures, and the binding constant was also determined by the method of fluorescence quenching.
The two methods yielded similar results. It indicated that the former method could be successfully applied to the determination
of BR. The results showed that the binding of BR to BSA induced conformational changes in BSA. Based on the theory of F?rster
energy transfer, the distance between BR and protein were calculated. According to the thermodynamic parameters, the main
binding force could be judged. The experimental results revealed that BSA and BR had strong interactions. The mechanism of
quenching belonged to static quenching and the main sort of binding force was van der Waals interactions and hydrogen bonds. 相似文献
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运用荧光光谱、紫外吸收光谱研究了Eu3+存在下盐酸头孢替安(Cefotiam Hydro-chloride,CH)与牛血清白蛋白(BSA)的相互作用.CH对BSA具有荧光猝灭作用,其猝灭机制为静态猝灭,BSA发射峰蓝移,二者之间的作用力主要为疏水作用和弱的静电作用.Eu3+的存在使得BSA发射峰蓝移程度降低,猝灭常数、结合常数、结合位点数减小,但没有改变CH对BSA的猝灭机制,热力学参数ΔH和ΔS都增大.从热力学参数的变化及Eu3+的竞争作用分析了Eu3+对CH与BSA作用影响的原因. 相似文献
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利用荧光光谱法,研究了1,4,7,10-四氮杂环十二烷合钴(Cyclen-Co(Ⅱ))配合物与牛血清白蛋白(BSA)的相互作用,用Stern-Volmer和Lineweaver Burk方程确定了其荧光猝灭机理为静态猝灭,计算得到了在不同温度和pH值条件下以及在聚氧乙烯(23)月桂醇醚(Brij35)、十六烷基三甲基溴化铵(CTAB)、十二烷基硫酸钠(SDS)3种表面活性剂胶束溶液中,Cyclen-Co(Ⅱ)和BSA作用的结合点位数、结合常数和热力学参数,研究结果表明,其作用力主要为氢键和范德华力。 相似文献