|
|||||
|
|
| 贝加因与牛血清白蛋白相互作用的分子光谱法研究 | |
| 引用本文： | 熊利敏,宋胜梅,张银堂,徐茂田.贝加因与牛血清白蛋白相互作用的分子光谱法研究[J].化学研究,2011,22(1):9-13. |
| 作者姓名： | 熊利敏 宋胜梅 张银堂 徐茂田 |
| 作者单位： | 1. 中南大学化学与化工学院,湖南,长沙,410083;商丘师范学院化学系,河南省纳米生物分析化学重点实验室培育基地,河南,商丘,476000;郑州大学化学系,河南,郑州,450001 2. 商丘师范学院化学系,河南省纳米生物分析化学重点实验室培育基地,河南,商丘,476000;郑州大学化学系,河南,郑州,450001 3. 商丘师范学院化学系,河南省纳米生物分析化学重点实验室培育基地,河南,商丘,476000 |
| 基金项目： | supported by the National Science Foundation of China(20775047 and 20905045) |
| 摘 要： | 利用荧光光谱和吸收光谱研究了贝加因与牛血清白蛋白(BSA)的相互作用,由Van't Hoff方程计算了反应的热力学参数,并根据Stern-Volmer方程计算了不同温度下的结合位点和结合常数.结果表明,贝加因可静态猝灭BSA的内源荧光;二者相互作用的焓变和熵变均大于零,说明疏水作用力是二者之间的主要作用力.与此同时,贝... |
| 关 键 词： | 贝加因 牛血清白蛋白 相互作用 分子光谱 |
Study of interaction between baicalein and bovine serum albumin by molecular spectrometry |
|
| XIONG Li-min,SONG Sheng-mei,ZHANG Yin-tang,XU Mao-tian.Study of interaction between baicalein and bovine serum albumin by molecular spectrometry[J].Chemical Research,2011,22(1):9-13. | |
| Authors: | XIONG Li-min SONG Sheng-mei ZHANG Yin-tang XU Mao-tian |
| Institution: | XIONG Li-min1,2,3,SONG Sheng-mei2,ZHANG Yin-tang2,XU Mao-tian1,3(1.Institute of Chemistry & Chemical Engineering,Central South University,Changsha 410083,Hunan,China,2.Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry,Department of Chemistry,Shangqiu Normal University,Shangqiu 476000,Henan,3.Department of Chemistry,Zhengzhou University,Zhengzhou 450001,China) |
| Abstract: | The interaction between baicalein and bovine serum albumin(BSA) was studied by means of fluorescence spectrometry and absorption spectrometry.The thermodynamic parameters of the reaction were calculated using Van't Hoff equation.Besides,the number of binding sites and the apparent binding constants at different temperatures were calculated from fluorescence quenching data using Stern-Volmer equation.Results indicate that baicalein is able to quench the intrinsic fluorescence of BSA via static quenching mech... |
| Keywords: | baicalein bovine serum albumin interaction molecular spectrometry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |