| 荧光光谱法研究辛硫磷与牛血清白蛋白的相互作用 |
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| 引用本文： | 吴汉东,史雪岩,李永丹.荧光光谱法研究辛硫磷与牛血清白蛋白的相互作用[J].分析试验室,2009,28(3). |
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| 作者姓名： | 吴汉东 史雪岩 李永丹 |
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| 作者单位： | 中国农业大学昆虫学系,北京,100193 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，教育部新世纪优秀人才支持计划，国家自然科学基金 |
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| 摘 要： | 用荧光光谱法研究了在生理pH条件下杀虫剂辛硫磷与牛血清白蛋白(BSA)的相互作用. 结果表明: 辛硫磷对BSA的荧光有较强的猝灭作用, 该猝灭属于静态猝灭. 根据猝灭结果求得了不同温度下辛硫磷与牛血清白蛋白结合作用的结合位点数、结合常数及反应热力学参数, 并据此确定它们之间主要的相互作用力为疏水作用力. 用同步荧光光谱法探讨了辛硫磷对BSA构象的影响.
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| 关 键 词： | 辛硫磷 牛血清白蛋白 相互作用 荧光光谱 杀虫剂 |
Study of interaction between phoxim and bovine serum albumin by fluorescence spectroscopy |
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| WU Han-dong,SHI Xue-yan,LI Yong-dan.Study of interaction between phoxim and bovine serum albumin by fluorescence spectroscopy[J].Chinese Journal of Analysis Laboratory,2009,28(3). |
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| Authors: | WU Han-dong SHI Xue-yan LI Yong-dan |
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| Institution: | Department of Entomology;China Agricultural University;Beijing 100094 |
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| Abstract: | The Interaction between phoxim which is a insecticide and bovine serum albumin (BSA) was studied by fluorescence spectroscopy at the physiological pH. The results showed that phoxim strongly quenched the fluorescence of BSA and it was the static quenching. The average number of binding sites and the binding constant for binding phoxim to BSA at different temperatures were determined. The thermodynamic parameters were also calculated. According to the analytical results, the binding pattern is considered to ... |
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| Keywords: | Phoxim Bovine serum albumin Interaction Fluorescence spectroscopy insecticide |
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