共查询到20条相似文献,搜索用时 125 毫秒
1.
气相色谱法直接测定动物组织中盐酸克仑特罗的残留量 总被引:8,自引:0,他引:8
1 实验部分1.1 仪器与试剂 HP6890气相色谱/氮磷检测器,超声波清洗器,离心机,组织匀浆机,吹氮浓缩仪,C18固相萃取小柱。 盐酸克仑特罗(又名:瘦肉精)标准储备液(1 0g/L):称取盐酸克仑特罗标准品(中国药品生物制品检定所)25 0mg,用0 01mol/L盐酸定容至25mL。 盐酸克仑特罗标准工作液(40.0mg/L):取1 0mL标准储备液,用NaOH(2mol/L)调pH至12,用5mL乙酸乙酯提取3次,合并提取液,并用乙酸乙酯定容至25mL。乙酸乙酯、三氯乙酸、氢氧化钠均为分析纯,盐酸为优级纯。1.2 实验方法 色谱条件 色谱柱:HP… 相似文献
2.
3.
4.
前不久,国际上第一个用单抗对盐酸克仑特罗(俗称瘦肉精)进行检测的试剂盒在张家港诞生,负责这一项目的张家港出入境检验检疫局与扬州大学畜牧兽医学院盐酸克仑特罗课题联合攻关小组已着手准备该课题的鉴定。 盐酸克仑特罗由于本身促生长和提高胴体瘦肉率的特性,长期以来一直被人们作为促生长添加剂使用,但由于其本身对心血管有毒性,在许多国家相继被禁止使用,我国农业部在1997年就颁布了有关法规禁止在动物饲料中添加克 相似文献
5.
6.
7.
建立了测定盐酸克仑特罗片剂含量的高效液相色谱法。选用Agilent Eclipse XDB-C8柱(4.6 mm×250 mm,5μm),流动相为甲醇-水(40∶60,含20 mmol.L-1磷酸二氢钾,以磷酸调至pH 4.0),流速为1.0 mL.min-1,检测波长240 nm,线性范围为0.50~50.0 mg.L-1(r=0.999 9),检出限为0.5 ng,回收率在98.5~102.0%之间,RSD为2.06%~3.07%(n=5)。 相似文献
8.
盐酸克仑特罗(Clenbuteroli Hydrochloridum)是一种平喘药,主要用于防治支气管哮喘和喘息性支气管炎。化学名称是1-(4-氨基-3,5-二氯苯基-2-叔丁氨基乙醇的盐酸盐,其结构式为 其制剂(片)中含量测定卫生部药品标准和山东省药品标准均采用重氮化—偶合比色法。盐酸克仑特罗在酸性(HCl液)介质中加亚硝酸钠重氮化后,再加盐酸萘乙二胺偶合生成偶氮化合物,在500nm波长处测吸收度。由于在重氮化过程中过量亚硝酸可使偶合试剂变色,干扰显色反应,因此需加氨基磺酸胺 相似文献
9.
采用盐酸为介质,以顺序注射-氢化物发生原子荧光法测定生产用水中的锑含量.通过正交实验确定了仪器工作参数,研究了介质浓度、KBH4浓度、硫脲一抗坏血酸加入量等因素对测定结果的影响,探讨了共存离子的干扰.在选定的最佳实验条件下,方法的检出限为0.011ng/mL,线性范围0~8ng/mL,相对标准偏差为2.78%~6.20%,加标回收率为91.0%~99.1%.该方法操作简便,灵敏度高,准确度好,与微波消解法相比较,两者无显著性差异. 相似文献
10.
11.
Uroš Grošelj Mojca Žorž Amalija Golobič Branko Stanovnik Jurij Svete 《Tetrahedron》2013,69(52):11092-11108
A new and simple synthesis of novel N-protected methyl 5-substituted-4-hydroxypyrrole-3-carboxylates, which exist in equilibrium with their 4-oxo tautomers, has been developed in two steps starting from N-protected α-amino acids. The key intermediates are enaminones, which can also be isolated, characterized, and used for the construction of other functionalized heterocycles, before they spontaneously decompose to pyrrole products. 4-Hydroxypyrroles are prone to partial aerial oxidation but can be efficiently alkylated or reduced to stable polysubstituted pyrrolidine derivatives. 相似文献
12.
The chemoselectivity in the intramolecular CH insertion of various diazosulfonamides has been experimentally studied. The results reveal that the aliphatic 1,4-, 1,5-, or 1,6-C(sp3)?H insertions of diazosulfonamides are not accessible, while the aromatic 1,5-C(sp2)?H insertion can be realized specifically by adjusting the diazo-adjacent group. In addition, the general chemoselectivities in the intramolecular CH insertions of diazosulfonyl compounds are summarized. Generally, diazosulfones undergo both aromatic 1,5-C(sp2)?H and aliphatic 1,5- and 1,6-C(sp3)?H insertions, while diazosulfonates undergo aliphatic 1,5- and 1,6-C(sp3)?H insertions. However, diazosulfonamides only undergo aromatic 1,5-C(sp2)?H insertion. 相似文献
13.
N-Heterocyclic carbene-palladacyclic complexes 3 were successfully achieved in a one-pot procedure under mild conditions. The structure of 3a was unambiguously confirmed by X-ray single crystal diffraction and it was an active catalyst in the Buchwald-Hartwig amination and α-arylation of ketones even at very low catalyst loadings (0.01?mol%). 相似文献
14.
Shashikant U. Dighe Surya K. Samanta Shivalinga Kolle Sanjay Batra 《Tetrahedron》2017,73(17):2455-2467
An efficient iodine-mediated oxidative Pictet-Spengler reaction in dimethyl sulphoxide (DMSO) using terminal alkynes as the 2-oxoaldehyde surrogate for the synthesis of aryl (9H-pyrido[3,4-b]indol-1-yl)methanones is described. The scope of the protocol includes the total synthesis of Fascaplysin, Eudistomins Y1 and Y2. The methodology is extended for preparing pyrrolo[1,2-a]-quinoxaline and indolo[1,5-a]quinoxaline derivatives. The utility of 1-aroyl-β-carbolines was demonstrated by performing palladium-catalyzed β-carboline directed ortho-C(sp2)-H functionalization of the phenyl ring with thiomethyl (SMe) group using DMSO as source and for accessing 4-aryl-canthin-6-ones. 相似文献
15.
In this Letter, we described a facile method for constructing fused bicyclic 1-arylpyrazol-5-one ring system. We employed various methylene-containing carboxylic acids as the substrates and proved that the pyrazolone ring closure requires activated methylene group in intermediate II. Accordingly, a series of structurally diversified, fused bicyclic 1-arylpyrazol-5-ones was prepared in moderate to high yields using the requisite substrates. 相似文献
16.
An efficient approach to the synthesis of highly congested di, penta and hexacyclic pyrazoles as well as imidazole fragment containing novel heterocyclic molecule has been developed through a carbanion induced transformation of suitably functionalized 2H-pyran-2-ones, benzo[h]chromene and thiochromeno[4,3-b]pyrans. Due to the presence of fluorescence, we report their prime application metal sensor as off/on switching in ferric ions. 相似文献
17.
Synthesis of substituted pyrrolo[1,2-a]pyrazines and pyrazino[1,2-a]indoles from the Morita-Baylis-Hillman derivatives of acrylates via saponification followed by Curtius reaction is described. 相似文献
18.
用正丁胺作为碳源,采用射频辉光放电制备碳膜,选用激光染料R6G和聚乙二醇混合液作为蒸气源,采用单源热蒸发,在蒸发室与染料同时沉积得到混合膜,用拉曼光谱和红外光谱分析了碳膜的结构和键合方式,分析表明:碳膜中存在胺基团和氢原子.混合膜的荧光谱测量结果表明,认为正丁胺对染料荧光谱的影响是因为胺基和氢原子的存在. 相似文献
19.
An efficient tandem approach for the selective synthesis of 4,5-dihydroimidazo[1,5-a]quinoxalines 6a–g and imidazo[1,5-a]quinoxalines 7a–h by the reaction of 2-imidazolyl anilines 4a–c with aryl aldehydes 5a–k under mild reaction conditions is described. Introduction of electron releasing alkyl groups in substrates 4a–b was found to be instrumental for the success of the reaction. 相似文献
20.
The Diels-Alder reactivity of 1,2-heteroborines (H4C4B(H)X, X?=?NH, PH, AsH; O, S, Se) has been computationally explored by means of Density Functional Theory (DFT) calculations. The influence of the HB?=?X fragment on the reactivity of the system has been quantitatively analyzed in detail by means of the so-called Activation Strain Model (ASM) of reactivity. It is found that the interaction between these species and the dienophile is significantly stronger than that computed for their all-carbon isoelectronic counterpart, benzene. In addition, the strain energy plays a key role in the observed reactivity trends. The role of the aromaticity strength of these heteroarenes on the reactivity is also assessed. 相似文献