共查询到18条相似文献,搜索用时 609 毫秒
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化学发光分析法应用进展 总被引:6,自引:0,他引:6
对近年来化学发光分析法的研究应用最新进展作了评述,包括化学发光试剂的类型,化学发光在无机、有机及药物分析中的应用,全文引用文献105篇。 相似文献
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碘和碘化物在鲁米诺化学发光分析体系中的应用 总被引:6,自引:0,他引:6
申金山 《理化检验(化学分册)》1998,34(3):132-134
化学发光是指在某些特殊的化学反应中,反应的中间体或产物由于吸收了反应释放的化学能而处于电子激发态,当其回到基态时伴随产生光辐射的现象。根据化学发光反应在某一时刻的发光强度或反应的发光总量来确定反应中相应组分含量的分析方法称作化学发光分析。鲁米诺化学发光体系是最具有代表性的经典分析体系之一,这种分析方法具有所用仪器设备简单、操作方便、线性范围宽、灵敏度高、易于实现自动化等诸多优点,常用来作为微量分析和痕量分析手段在分析领域中广泛应用。 相似文献
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研究了Eu(Ⅲ)对Luminol-H2O2-Cr(Ⅲ)体系化学发光的熄灭效应,在此基础上建立了铕的流动注射化学发光分析法。分析灵敏度高,线性范围宽,仪器设备简单,操作方便。 相似文献
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中国化学发光分析进展 总被引:6,自引:0,他引:6
化学发光分析是近年来获得迅速发展的一类高灵敏度的微量及痕量分析技术,应用广泛,前景诱人,是当前分析化学研究中的一个新热点。我国的研究工作虽起步较晚,但发展速度很快,本文就我国学者在这一领域的研究进展作一全面回顾。 相似文献
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化学发光联用技术在兽药残留分析中的应用进展 总被引:7,自引:0,他引:7
本文对近年来化学发光联用技术在兽药残留分析中的应用进展进行了评述,主要包括化学发光免疫分析、分子印迹-化学发光分析、微流控芯片-化学发光检测等,引用文献31篇. 相似文献
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高锰酸钾-鲁米诺反应体系中碱土金属离子Mg2+,Ca2+,Sr2+,Ba2+化学发光行为研究 总被引:1,自引:0,他引:1
将被认为没有化学发光活性的第二主族(碱土金属)离子Mg^2 ,Ca^2 ,Sr^2 ,Ba^2 溶液注入到已充分反应的高锰酸钾与鲁米诺混合液中时,又发生了新的化学发光反应,并检测到强的化学发光信号.在对有关反应的动力学性质、化学发光光谱、紫外可见光谱及其它一系列实验研究的基础上,提出了可能的化学发光反应机理.同时,优化了反应条件,评价了这一反应用于Mg^2 ,Ca^2 ,Sr^2 ,Ba^2 分析的可行性. 相似文献
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化学发光免疫分析技术发展迅速,具有特异性强、灵敏度高、线性范围宽、操作简单、成本底、检测时间短、易于实现自动化等优点,在农残检测领域得到广泛应用。以化学发光免疫分析为基础,介绍了吖啶酯类、鲁米诺类、咪唑类、苯酚类及芳基草酸酯类5种化学发光剂,及化学发光免疫分析法、荧光化学发光免疫分析法、化学发光酶联免疫分析法和电化学发光免疫分析法4种常见的分析方法,重点综述了化学发光免疫分析技术在农药残留检测中的应用,并对该领域的研究前景进行了展望,旨在为农药残留检测相关研究提供参考。 相似文献
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化学发光法在药物分析中的应用 总被引:6,自引:0,他引:6
化学发光法因具有设备简单、灵敏度高等特点而被广泛采用。文中对近年涉及药物、药物在人体中的代谢产物及生命相关物质的化学发光分析进行了评述,引用文献87篇。 相似文献
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In this review, the recent evolution and the state of the art of photochemical reactions coupled with chemiluminescence processes are presented. Different chemiluminescence systems have been considered together with suitable photochemical derivatization processes that can affect either the analyte of interest or even the chemiluminogenic reagent, producing some derivatives able to participate more efficiently in the CL reactions and enhancing the CL emission. The on-line integration of the photochemical reactions as well as the coupling of this resulting photoinduced chemiluminescence (PICL) method with dynamic analytical systems, such as flow injection analysis, liquid or gas chromatography and capillary electrophoresis, have been discussed. Important applications of PICL have been proposed in environmental, pharmaceutical and food analysis. 相似文献
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The present paper reviews the developments and applications of chemiluminescence detection with HPLC and CE in pharmaceutical and biomedical analysis. The chemiluminescence systems, chemiluminogenic reagents and derivatization reagents, improvements in instrumental design as well as their contributions to the practical applications, are all presented. The advantages and limitations of current detection methodology and future prospects for improvement are briefly discussed. 相似文献
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Kazuhiko Tsukagoshi Yukihiro Shikata Riichiro Nakajima Masaharu Murata Mizuo Maeda 《Analytical sciences》2002,18(11):1195-1198
We developed capillary electrophoresis with a chemiluminescence detector using a polymer solution as the separation medium for the analysis of biopolymers, such as DNA and protein. A peroxyoxalate chemiluminescence reagent of bis(2,4,6-trichlorophenyl)oxalate was used together with fluorescein-labeling reagent. When a migration buffer solution containing carboxylmethylcellulose was used, the flow-type chemiluminescence detection cell was found to give a better resolution than the batch-type one. Fluorescein-labeled adenosine triphosphate of 1.0 x 10(-4) M was examined by means of capillary electrophoresis with absorption (260 nm), fluorescence (ex. 496 nm and em. 517 nm), and chemiluminescence detectors. The chemiluminescence detection showed the highest sensitivity among them; the S/N ratios obtained by absorption, fluorescence, and chemiluminescence detections were 4, 38, and 130, respectively. Fluorescein-labeled DNA was prepared through a polymerase chain reaction using fluorescein-labeled deoxyadenosine triphosphate. A mixture of the labeled DNA fragments (500, 600, 700, 800, 900, and 993 bp) was successfully separated and detected by the present system. A mixture of proteins (lysozyme, cytochrome C, and ribonuclease A) which were labeled with fluorescein isothiocyanate was also separated and detected. 相似文献
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Naoya Kishikawa Nobuhiro Ohkubo Kaname Ohyama Kenichiro Nakashima Naotaka Kuroda 《Analytical and bioanalytical chemistry》2009,393(4):1337-1343
A sensitive and selective chemiluminescence assay for the determination of quinones was developed. The method was based on
generation of reactive oxygen species through the redox reaction between quinone and dithiothreitol as reductant, and then
the generated reactive oxygen was detected by luminol chemiluminescence. The chemiluminescence was intense, long-lived, and
proportional to quinone concentration. It is concluded that superoxide anion was involved in the proposed chemiluminescence
reaction because the chemiluminescence intensity was decreased only in the presence of superoxide dismutase. Among the tested
quinones, the chemiluminescence was observed from 9,10-phenanthrenequinone, 1,2-naphthoquinone, and 1,4-naphthoquinone, whereas
it was not observed from 9,10-anthraquinone and 1,4-benzoquinone. The chemiluminescence property was greatly different according
to the structure of quinones. The chemiluminescence was also observed for biologically important quinones such as ubiquinone.
Therefore, a simple and rapid assay for ubiquinone in pharmaceutical preparation was developed based on the proposed chemiluminescence
reaction. The detection limit (blank + 3SD) of ubiquinone was 0.05 μM (9 ng/assay) with an analysis time of 30 s per sample.
The developed assay allowed the direct determination of ubiquinone in pharmaceutical preparation without any purification
procedure.
Figure Chemiluminescence generated through the redox cycle of quinone 相似文献
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化学发光分析法的发展与应用 总被引:7,自引:1,他引:6
近几年来,化学发光分析作为一种高灵敏度的痕量分析方法,发展迅速,不断深入到有机、药物、食检、环境和材料科学等各个领域.用于化学发光分析的发光体系也日益增多.介绍了很多近年来初步建立起来的新的化学发光体系,并对其应用作了较为详细的评述. 相似文献
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We developed a capillary electrophoresis with chemiluminescence detection system using 2-methyl-6-p-methoxyphenylethynylimidazopyrazinone as a chemiluminescence reagent for determination of antioxidants of superoxide anions. 2-Methyl-6-p-methoxyphenylethynylimidazopyrazinone reacted with superoxide anions generated through the reaction of hypoxanthine and xanthine oxidase, and then emitted chemiluminescence. Suppression of the chemiluminescence in the presence of antioxidants for superoxide anions was introduced as a detection principle for antioxidants into the capillary electrophoresis with chemiluminescence detection system. After optimizing the analytical conditions, various antioxidants, such as superoxide dismutase, nitroblue tetrazolium, ascorbic acid, and catechin, were subjected to the present system. They gave negative peaks due to the quenching effect; the detection limits of superoxide dismutase, nitroblue tetrazolium, ascorbic acid, and catechin were 1, 100, 100, and 10 μM, respectively (S/N = 2). A model sample consisting of superoxide dismutase and nitroblue tetrazolium was satisfactorily separated and detected within ca. 10 min. We also applied the present system to analysis of catechin in green tea as a real sample. 相似文献