固相萃取/超高效液相色谱－串联质谱法测定儿童化妆品中新康唑等4种抗生素

建立了测定儿童化妆品中新康唑等4种抗生素的超高效液相色谱－串联质谱法（UPLC－MS/MS）。样品经70%乙腈水溶液超声提取后,使用SCX固相萃取小柱净化,以0.1%甲酸水和乙腈作为流动相,经Waters ACQUITY BEH C18（2.1 mm × 100 mm,2.5 μm）色谱柱分离,使用多反应监测模式（MRM）进行测定。结果表明,4种抗生素线性关系良好,相关系数（r）均为0.999,方法检出限（LOD）和定量下限（LOQ）分别为0.005 ~ 0.250 μg/g和0.010 ~ 0.500 μg/g。4种抗生素在3种基质和3个加标水平的平均回收率为89.3% ~ 113%,相对标准偏差（RSD）为0.90% ~ 7.2%。该方法高效、准确、特异性好,满足新康唑等4种抗生素在儿童化妆品中的测定需求。     

Resolution enhancement with improved range Doppler algorithm in high numerical aperture OCT

The improved range Doppler algorithm is proposed to abate the trade-off between resolutions and depth of focus in spectral domain optical coherence tomography.By considering the finite beam width and the shape of the wavefronts produced by the Gaussian beam,a physical diffraction model is presented to simulate the light propagation process in the sample.The two-dimensional processing of the spectrum data is decomposed into two one-dimensional processings of Stolt transform and matched filter.Experimental results show that image reconstruction can be achieved.The transverse and axial resolutions are both improved significantly,especially in the out-of-focus range,and the resolutions are almost equivalent throughout the entire region of interest.     

Light-shift induced by two unbalanced spontaneous decay rates in EIT (CPT) spectroscopies under Ramsey pulse excitation

Light shift is important and inevitably affects the long-term stability of an atomic clock. In this work, considering two unbalanced branches of the spontaneous decay rate in a three-level system, we studied the frequency shifts of electromagnetically induced transparency (EIT) and coherent population trapping (CPT) clocks operating under the pulse sequence regime by numerically solving the Liouville density matrix equations. The results show that the frequency shifts are larger when the two branches of spontaneous emission rate are not equal compared to the equal case. In addition, in EIT-Ramsey, the effect of the unbalanced branches of the spontaneous decay rate and relaxations of low-energy states on the frequency shift is greater than that of Rabi frequency. In CPT-Ramsey, the relaxations of low-energy states play a dominant role in frequency shift.     

Temperature Dependence of Dark Lines in Coherent Population Trapping

We measure the signal amplitude and linewidth of a dark line in coherent population trapping in the Rb vapour cell filled with mixed buffer gas N2 and Ar as a function of cell temperature. We find that the dark line signal amplitude increases with temperature up to a maximum at 49℃ and then drops at higher temperatures due to quenching effects of N2. The linewidth of the dark line remains basically constant, at 1080 Hz. We also measure the linewidth of the dark line as a function of laser intensity. The linewidth increases linearly with laser intensity. An intrinsic linewidth （FWHM=896 Hz at 3.4 GHz） of the Rb cell is obtained.     

Bichromatic Coherent Population Trapping Under Hanle Configuration

We report the observation of bichromatic coherent population trapping under the transition ^87 RbFg = 1 → Fc = 0 with two linearly polarized laser beams by means of a Hanle effect configuration. The mechanism behind this effect is identified, and numerical solutions for the pump rates equations are presented.     

超冷玻色气体中电磁感应透明和慢光现象中的原子相互作用效应

我们研究了原子之间的相互作用对超冷玻色原子气体中电磁感应透明性质和光的群速的影响.我们考虑Λ型的原子结构.最初原子凝聚在其中的一个基态上,凝聚体原子用平均场理论来描述,原子之间的相互作用近似为两体的s-波散射长度,原子之间的碰撞不引起原子的电子跃迁.     

Transparency and Strong Gain Without Population Inversion in Photonic Bandgap Crystals

Without using the weak-field approximation,we investigate the transient properties of a Λ-type atom with one transition near resonant with a photonic bandgap edge.Whatever the initial state of the atom is,the atom can become transparent to a probe field,and strong gain without population inversion is also possible if the atom has been pre-excited.The defect mode formed by atomic doping can have a strong effect on the absorption properties of the atom.     

含有割点的图的最小特征值

Let ψ be a certain set of graphs.A graph is called a minimizing graph in the set ψ if its least eigenvalue attains the minimum among all graphs in ψ.In this paper,we determine the unique minimizing graph in ψn,where ψn denotes the set of connected graphs of order n with cut vertices.     

基于蒙特卡罗模拟的超声辅助OCT成像研究

通过蒙特卡罗模拟方法研究了超声波提高OCT成像质量的机理,以宏观经典理论和微观量子理论分别阐述了光和超声的相互作用,建立了存在超声辅助时光在生物组织中传播的模型,模拟了有超声辅助和无超声辅助两种情况下生物组织多次散射光的分布.模拟结果表明,与光平行射入生物组织的超声波能够使OCT成像系统中的多次散射光降低80%,携带组织信息的单次散射光在组织返回光中所占比重增大为无超声辅助时的2倍,从而增大了OCT成像的探测深度及图像对比度.     

纳米探针芯片技术用于微量乙肝病毒DNA的检测

利用两组探针修饰的微粒:(1)表面标记有可与待测乙肝病毒(HBV) DNA另一端结合的纳米金探针1(信号探针)以及可与信号探针部分结合的纳米金探针2(检测探针);(2)表面标记有可与待测HBV DNA一端结合的磁珠探针(捕捉探针1).检测靶HBV DNA时,磁珠探针与信号探针在液相中可分别与HBV DNA靶序列一端结合最终形成三明治样结构.再以磁场将三明治样复合物从反应液中分离,以DTT溶液将信号探针从纳米金颗粒上洗脱.洗脱后的信号探针数量反映靶基因的多寡,信号探针一段与预先点样的基因芯片上的捕捉探针2结合,检测探针与信号探针另一段相结合,最后用银染液将检测探针显色从而得到靶目标DNA相对定量信息.结果表明,本检测方法的检测灵敏度达到10-15 mol/L水平.检测时间少于1.5 h,检测结果与HBV DNA水平呈现较好的线性关系且无假阳性结果;本方法有望用于乙肝病人血清中HBV DNA的快速筛测及其它微生物基因的检测.