|
|||||
|
|
| 纳米探针芯片技术用于微量乙肝病毒DNA的检测 | |
| 引用本文： | 汪毅,毛红菊,臧国庆,张宏莲,金庆辉,赵建龙.纳米探针芯片技术用于微量乙肝病毒DNA的检测[J].分析化学,2010,38(8). |
| 作者姓名： | 汪毅 毛红菊 臧国庆 张宏莲 金庆辉 赵建龙 |
| 作者单位： | 1. 上海交通大学附属第六人民医院,上海,200062;中国科学院上海微系统与信息技术研究所,上海,200050 2. 中国科学院上海微系统与信息技术研究所,上海,200050 3. 上海交通大学附属第六人民医院,上海,200062 |
| 基金项目： | 国家卫生部重大专项，上海市科委项目 |
| 摘 要： | 利用两组探针修饰的微粒:(1)表面标记有可与待测乙肝病毒(HBV) DNA另一端结合的纳米金探针1(信号探针)以及可与信号探针部分结合的纳米金探针2(检测探针);(2)表面标记有可与待测HBV DNA一端结合的磁珠探针(捕捉探针1).检测靶HBV DNA时,磁珠探针与信号探针在液相中可分别与HBV DNA靶序列一端结合最终形成三明治样结构.再以磁场将三明治样复合物从反应液中分离,以DTT溶液将信号探针从纳米金颗粒上洗脱.洗脱后的信号探针数量反映靶基因的多寡,信号探针一段与预先点样的基因芯片上的捕捉探针2结合,检测探针与信号探针另一段相结合,最后用银染液将检测探针显色从而得到靶目标DNA相对定量信息.结果表明,本检测方法的检测灵敏度达到10-15 mol/L水平.检测时间少于1.5 h,检测结果与HBV DNA水平呈现较好的线性关系且无假阳性结果;本方法有望用于乙肝病人血清中HBV DNA的快速筛测及其它微生物基因的检测. |
| 关 键 词： | 乙肝病毒DNA 纳米金探针 生物条形码扩增 纳米探针芯片 |
Detection of Hepatitis B Virus Deoxyribonucleic Acid Based on Gold Nanoparticle Probe Chip |
|
| WANG Yi,MAO Hong-Ju,ZANG Guo-Qing,ZHANG Hong-Lian,JIN Qing-Hui,ZHAO Jian-Long.Detection of Hepatitis B Virus Deoxyribonucleic Acid Based on Gold Nanoparticle Probe Chip[J].Chinese Journal of Analytical Chemistry,2010,38(8). | |
| Authors: | WANG Yi MAO Hong-Ju ZANG Guo-Qing ZHANG Hong-Lian JIN Qing-Hui ZHAO Jian-Long |
| Institution: | WANG Yi1,2,MAO Hong-Ju2,ZANG Guo-Qing1,ZHANG Hong-Lian2,JIN Qing-Hui2,ZHAO Jian-Long2 1(Department of Infection,the Sixth Peoples Hospital Affiliated to Shanghai Jiaotong University,Shanghai 200233) 2(Shanghai Institute of Microsystem and Information Technology,Chinese Academy of Sciences,Shanghai 200050) |
| Abstract: | A nanoparticle-based bio-barcode amplification (BCA) approach was developed for the sensitive detection of hepatitis B virus (HBV) DNA. The BCA approach employed two sets of particles: (1) two-component oligonucleotide-modified gold nanoparticles (AuNPs): one oligonuc-leotide probe called signal probe (barcode DNA) is partial complementary with the target sequence of HBV DNA and another one called detection probe is partial complementary with the signal probe; (2) single component oligonucleotide modified m... |
| Keywords: | Hepatitis B virus deoxyribonucleic acid Gold nanoparticle probes Bio-barcode amplification nanoparticle probe chip-based detection |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |