超声调制介质中漫散射光自相关性质研究

研究了超声调制随机介质中漫散射光自相关性质,推导出均匀无限介质中有超声场调制时漫散射光时间自相关函数的表达式,讨论了调制参数、调制幅度及其衰减与超声参数、光散射参数等的关系.用Monte Carlo方法研究了随机介质中隐含异物成像的可行性.结果表明,正常生物组织和病变生物组织的超声调制自相关函数有明显的差别,该方法为光学医学诊断提供了一种新参考 关键词： 自相关 漫散射光 超声调制 光学成像     

复谱频域OCT成像的研究

采用频域技术的OCT系统，深度扫描信息由背向散射光谱的傅立叶反变换获得，简化了轴向扫描过程，从而使快速OCT成像成为可能．为了实现复杂生物组织的OCT快速成像，消除谱频域OCT的“混叠”现象、重建样品的真实层析结构，本文引入了相移干涉技术，构建了一套满量程复谱频域OCT实验系统，并为系统设计了特殊的分束镜和移相器，为最终实现复杂生物组织的OCT快速成像提供了条件．     

超声波光生物制氢反应器启动及产氢特性

实验研究了超声波光生物制氢反应器的启动工艺以及反应器的产氢特性,探讨了超声时间和超声功率对反应器产氢性能的影响。超声波光生物反应器的启动实验进行了96 h,此时反应器光合细菌生物量和反应液pH值趋于稳定,启动完成。在系统稳定运行后,随着超声时间、超声功率的增大,超声波光生物制氢反应器的产氢速率和产氢得率呈先增加后降低的趋势,然而葡萄糖去除率却随着超声时间的增加而增大。     

视细胞浓度分布对散射光偏振性的影响

人眼视网膜是一种具有高散射特性的生物组织。基于米氏散射理论研究光在视网膜组织中的传输过程对生物组织散射成像具有重要意义。根据解剖学上视网膜结构与细胞分布的特点,引入分子动力学领域的静态结构因子修正辐射传递理论,并采用离散坐标法求解视细胞对散射光偏振性的影响。结果表明散射光偏振性取决于视细胞尺寸与浓度分布等因素。在不同的极角和光程位置,散射光束的偏振性呈现波动性变化,视锥细胞核比视杆细胞核具有更强的散射特性,散射光束的偏振特性变化随散射角的增大而增大。     

距离选通成像系统分辨力模拟测试装置的设计

距离选通成像系统采用的是脉冲激光主动照明与ICCD选通技术相结合的方法。为了在室内模拟测试在一定大气条件下距离选通成像系统的分辨力,设计了一套模拟测试装置。以辐射传输理论为基础,建立了大气距离选通成像系统后向散射光和信号光的模型。该模型给出了后向散射光和信号光在光电阴极面上照度随时间的变化规律。采用两个模拟光源,分别模拟了信号光和后向散射光。采用阴极面等效辐射照度法,给出了模拟光源功率的方法。以S 20光电阴极的像增强器与CCD的耦合为例,计算了模拟光源的功率。     

OCT系统对人体牙齿组织的非失真成像深度的研究

利用基于蒙特卡罗方法的牙组织光学相干层析(OCT)成像模型,研究了不同牙组织的OCT非失真成像深度。通过模拟入射高斯光束以及光在牙组织中的传输,分别获得了单层牙釉质、单层牙本质以及两层牙组织结构的二维仿真OCT图像,与实验结果具有定性的一致性。通过分析二维仿真OCT图像所对应的一维OCT信号,分别得到了三种牙组织结构的平均非失真成像光学深度。研究结果表明,OCT系统对牙齿组织的非失真成像光学深度在150~2400μm之间,其中牙釉质的非失真成像深度要远大于牙本质的成像深度。所得的结果对于在实验中利用OCT图像对组织结构有效信息进行判断具有一定的参考价值。     

距离选通成像系统分辨率模拟测试装置

距离选通成像系统采用脉冲激光主动照明与ICCD选通技术相结合. 为了在室内模拟测试一定大气条件下距离选通成像系统的分辨率,设计了一套模拟测试装置. 采用两个模拟光源,分别模拟信号光和后向散射光. 结合大气距离选通成像系统后向散射光和信号光模型的研究结果,采用阴极面等效辐射照度法,给出了模拟光源功率的计算方法和功率范围的确定方法,以S-20光电阴极的像增强器与CCD耦合为例,计算了模拟光源功率.     

光学滤波与频谱分析

依据光声效应产生的超声波具有波的特性,借鉴光学成像理论,提出了一种新的光声信号成像模式一利用声透镜直接成像。以声场复振幅来描述声场分布,根据基尔霍夫衍射理论,从理论上推导了声透镜的脉冲响应,并通过实验得到了生物组织的功能成像。     

烟雾环境下的偏振差成像方法

基于激光主动成像的烟雾环境下的目标探测具有重要研究意义,有效抑制光的散射作用是提高烟雾环境下成像质量的关键。根据光的偏振理论,仿真分析了光子单次散射的偏振特性与散射角的变化关系,并利用蒙特卡罗方法模拟了光在烟雾环境中传输的偏振特性,提出了利用偏振差法来抑制光散射作用,最后进行了简单的成像实验。结果表明,光在烟雾中传输时散射光主要由小角度散射光构成,仍然保留了初始激光的部分偏振特性,相比于直接灰度成像,偏振差法能够有效滤除小角度散射光的影响,增强成像目标的细节信息和图像的对比度。     

S波段微波热致超声成像系统研究

微波热致超声成像技术通过向物体发射微波脉冲, 导致物体吸收电磁波温度迅速升高, 产生瞬时压力波, 从而激发产生超声波信号, 通过传感器对产生的超声波信号进行采集并成像, 最终还原了反映物体吸收电磁波能量特性的图像, 由于此方法兼具了微波成像的高对比性和超声成像的高分辨率特点, 理论上验证了热声成像技术对早期乳腺肿瘤检测的可行性. 本实验兼顾系统成像深度和分辨率, 采用S波段的微波脉冲信号源对物体进行辐射, 利用圆形扫描方式对待测物体进行检测, 同时为了更好的验证成像性能, 本实验同时使用了肿瘤仿体及实际生物组织进行成像实验. 通过实验分析, 验证了该系统对肿瘤仿体和生物组织检测的有效性, 以及系统的高分辨率和高对比度特性, 为早期乳房肿瘤检测提供了进一步的理论支撑.     

Optical coherence tomography

Optical Coherence Tomography (OCT) is a new technology for performing high-resolution cross sectional imaging. OCT is analogous to ultrasound imaging, except that it uses light instead of sound. OCT can provide cross sectional images of tissue structure on the micron scale in situ and in real time. OCT functions as a type of optical biopsy and is a powerful imaging technology for medical diagnostics because unlike conventional histopathology which requires removal of a tissue specimen and processing for microscopic examination, OCT can provide images of tissue in situ and in real time. OCT can be used where standard excisional biopsy is hazardous or impossible, to reduce sampling errors associated with excisional biopsy, and to guide interventional procedures.     

基于光学相干层析术的组织光学性质测量

通过在光学相干层析成像(Optical Coherence Tomography,OCT)模型中考虑多重散射的影响,提出了一种提取出不同深度处的组织光学性质,包括散射系数和各向异性因子的算法.当把吸收系数作为算法中的第三个拟合参量时,能减少算法拟合过程中由于吸收作用的影响而带来的误差.基于包含吸收因子的OCT扩展模型,改进的算法也能够提取出吸收系数.这些算法的有效性通过对聚苯乙烯微球溶液样品的测量实验得到验证.     

基于受激辐射信号的谱域光学相干层析分子成像方法

鉴于不同生理病理状态下组织复折射率实部的变化不大,传统光学相干层析(OCT)成像技术在分子特异性识别上存在先天不足.为此,本文提出了基于受激辐射信号的OCT成像方法,可在实现传统散射成像的同时,实现基于受激辐射信号的分子成像.在超高分辨率谱域OCT系统的基础上,通过增设光谱分光与调制抽运光支路,建立了基于单宽谱光源的抽运探测谱域OCT系统,详细推导了调制抽运下受激辐射信号的获取与成像公式.利用搭建的抽运探测谱域OCT系统,实现了瞬态受激辐射信号的相干探测.基于同时获取的受激辐射OCT信号和传统OCT信号,成功重构了氮化物粉末构建样品的基于受激辐射信号的分子对比OCT图像.     

Recent advances in wavefront shaping techniques for biomedical applications

Due to the highly inhomogeneous distributions of refractive indexes, light propagation in complex media such as biological tissue experiences multiple light scattering events. The suppression and control of multiple light scattering events are investigated because they offer the possibility of optical focusing and imaging through biological tissues, and they may open new avenues for diagnosis and treatment of several human diseases. In order to provide insight into how new optical techniques can address the issues of multiple light scattering in biomedical applications, the recent progress in optical wavefront-shaping techniques is summarized.     

Auto-correlation Properties of Scattering Light in Ultrasound-modulated Random Media

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Auto-correlation Properties of Scattering Light in Ultrasound-modulated Random Media

In this paper, the auto-correlation properties of scattering light in random media modulated by ultrasound were studied. The expression of temporal auto-correlation function of scattering light amplitude in the ultrasound-modulated media was presented. The results show that the auto-correlation function is modulated as the ultrasound is introduced into the media and the modulation amplitude decays with correlation time. The influences of ultrasound amplitude, Brownian diffusion coefficient, scattering and absorption coefficients on auto-correlation function were discussed. The auto-correlation imaging of an object hidden in random media was also studied by the use of Monte Carlo simulations.     

Light transport in laser irradiated turbid media, application in medicine and image processing

Much effort is being made in the study of light propagation in human tissue due to the development and wide use of lasers for surgical and therapeutic applications. When a picosecond light pulse is incident on biological tissue, part of the light is reflected back owing to specular surface reflection and the rest of the light penetrates the tissue and encounters multiple scattering and absorption processes. An analysis has been presented for modeling of light distribution in human tissue. The influence of the refractive index, scattering phase function and scattering albedo on light distribution in laser irradiated tissue has been demonstrated.     

Functional optical coherence tomography for detecting neural activity through scattering changes

We have demonstrated functional optical coherence tomography (fOCT) for neural imaging by detecting scattering changes during the propagation of action potentials through neural tissue. OCT images of nerve fibers from the abdominal ganglion of the sea slug Aplysia californica were taken before, during, and after electrical stimulation. Images acquired during stimulation showed localized reversible increases in scattering compared with those acquired before stimulation. Motion-mode OCT images of nerve fibers showed transient scattering changes from spontaneous action potentials. These results demonstrate that OCT is sensitive to the optical changes in electrically active nerve fibers.     

Extraction of optical scattering parameters and attenuation compensation in optical coherence tomography images of multilayered tissue structures

A recently developed analytical optical coherence tomography (OCT) model [Thrane et al., J. Opt. Soc. Am. A 17, 484 (2000)] allows the extraction of optical scattering parameters from OCT images, thereby permitting attenuation compensation in those images. By expanding this theoretical model, we have developed a new method for extracting optical scattering parameters from multilayered tissue structures in vivo. To verify this, we used a Monte Carlo (MC) OCT model as a numerical phantom to simulate the OCT signal for heterogeneous multilayered tissue. Excellent agreement between the extracted values of the optical scattering properties of the different layers and the corresponding input reference values of the MC simulation was obtained, which demonstrates the feasibility of the method for in vivo applications. This is to our knowledge the first time such verification has been obtained, and the results hold promise for expanding the functional imaging capabilities of OCT.