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以纳米氧化铝/壳聚糖无机-有机生物复合膜为基质吸附纳米金-HRP,构建了新型酶传感器,将其用于H2O2的电化学检测。运用循环伏安法(CV)研究了该传感器的性能,并对实验条件进行了优化。在优化实验条件下,传感器对H2O2在0.3~270μmol/L浓度范围内与电流呈线性关系,检出限为0.1μmol/L。除高浓度的抗坏血酸有轻微干扰外,其他可能的共存物质如葡萄糖和各种氨基酸,以及生物相关的常见阴、阳离子对检测H2O2无明显干扰。酶促催化反应的表观米氏常数为0.13 mmol/L。该传感器的稳定性和重复性良好,用于测定医用消毒水中H2O2的含量,结果满意。该生物传感器具有快速、简单、成本低等优点,可推广应用到其它酶生物传感器的构建。 相似文献
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An integration depending on a parameter to the compression of a thick workpiece has been obtained.For the conventional prevailing numerical formulaa definite functional relationship betweenφand y is found.Therefore a parametric integration can be used to get an analytical solution.Take the slip line field for1/h=0.121 as an example,the analytical solution is basically the same as the prevailing numerical one.It is justified theoretically that for the slip line field a parametric integration is perfectly possible for a satisfactory analytical solution. 相似文献
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