基于TCK模型的非贯通节理岩体动态损伤本构模型

提出在岩体动态损伤本构模型中应同时考虑宏、细观缺陷;基于能量原理和断裂力学理论推导得出了同时考虑节理几何及力学特征的宏观损伤变量(张量)的计算公式;基于综合考虑宏、细观缺陷的复合损伤变量(张量)及完整岩石动态损伤Taylor-Chen-Kuszmaul(TCK)模型,建立了相应的单轴压缩下节理岩体动态损伤本构模型;利用该模型讨论了节理内摩擦角及节理长度对岩体动态力学特性的影响规律。研究表明,试件动态峰值强度随着节理内摩擦角的增大而增大,随着节理长度的增加而减小。     

双频光栅傅里叶变换轮廓术频谱混叠研究

讨论了用双频光栅方法对包含突变成份的物体产生的变形条纹进行傅里叶变换时,可能会出现频谱混叠问题.推出了低频光栅的频谱f1与高频光栅的频谱f2不相互混叠的条件,分析了探测器非线性会引起同一光栅间频谱发生混叠情况.考虑到通常情况下低频光栅频谱f1与高频光栅频谱f2的混叠起主要作用,因此在该情况下用计算机仿真与实验验证了：当f2<2f1时,f1与f2相互混叠,物体面形难以恢复;当f2>2f1时,f1与f2不相互混叠,物体面形恢复得很好.     

瑞利散射多普勒测风激光雷达的校准

 在进行实际风速测量之前,对于新研制的测风激光雷达系统进行校准,可以验证并提高风速测量的准确性。根据瑞利散射多普勒激光雷达的测量原理,提出了利用瑞利散射谱和米散射谱之间的关系,采用运动硬目标实现对瑞利散射多普勒测风激光雷达进行校准的方法。设计了对瑞利散射多普勒测风激光雷达进行校准的实验系统,并给出了详细的校准步骤。     

A comprehensive application: Molecular docking and network pharmacology for the prediction of bioactive constituents and elucidation of mechanisms of action in component-based Chinese medicine

Traditional Chinese medicine (TCM) has been used for more than 2000 years in China. TCM has received wide attention recently due to its unique charm. At the same time, its main obstacles have attracted wide attention, including vagueness of drug composition and treatment mechanism. With the development of virtual screening technology, more and more Chinese medicine compounds have been studied to discover the potential active components and mechanisms of action. Molecular docking is a computer technology based on structural design. Network pharmacology establishes powerful and comprehensive databases to understand the relationship between TCM and disease network. In this review, emergent uses and applications of two techniques and further superiorities of the two techniques when embarked to boil down into a tidy system were illustrated. A combination of the two provides a theoretical basis and technical support for the construction of modern TCM based on the compatibility of components and accelerates the realization of two basic elements as well, including the clearness of the pharmacodynamic substances and explanation of the effect of TCM.     

STUDY ON DEBONDING FAILURE MODEL OF POLYUREA-BASED COATING WITH HIGH VELOCITY WATER FLOW BASED ON COHESIVE ZONE MODEL

冲磨和空蚀破坏是水利水电设施最为常见的病害之一,严重影响水利水电设施的安全运行和效益发挥. 泄洪建筑物通常喷涂聚脲基涂层来提高抗冲耐磨性能,但在泄洪高速水流速度作用下抗冲磨聚脲基涂层的剥离破坏机理的研究尚属空白. 本文基于高速水流的流态形式,提出了高速水流对泄洪建筑物的力学作用模型,水流作用对泄洪建筑物的载荷主要包括拖曳力、冲击力、脉动力和上浮力;采用内聚力模型表征聚脲基涂层与泄洪建筑物防护体界面的剥离破坏过程,建立了高速水流聚脲涂层的剥离破坏模型, 给出了模型的有限元形式方程、本构关系以及损伤起始原则、演化原则和接触碰撞模型. 通过聚脲涂层与混凝土基底的剥离破坏试验,分析了不同剥离倾角下界面剥离破坏的拉应力与倾角之间的变化规律,得到了聚脲涂层剥离破坏过程中应力-$\!$-位移变化关系. 根据剥离破坏试验计算了界面剥离破坏断裂模型参数,采用数值方法对模型进行了验证,试验结果与模型计算结果吻合良好,为泄洪建筑物的抗冲耐磨设计提供理论依据.     

矩形微通道内两相流流阻压降特性的可视化研究

以去离子水和质量分数为0.3%的水基纳米流体为工质,在当量直径为1.241mm的矩形微通道内进行了两相流流动沸腾的实验研究,并借助高速摄像仪对矩形微通道内流型随着质量流量及热流密度的变化进行了观察分析。实验结果表明:单位长度上的两相摩擦压降会随着质量流速的提升而提高;单位长度上的两相摩擦压降会随着热流密度的增大而升高;减小质量流速和提高其热流密度均会加快气泡的产生并提高气泡的脱离直径,当热流密度增大到一定程度时,通道内几乎为环状流与液态单相流交替出现,且环状流占周期中的较长时间。     

Enhanced electrochemical activity of redox-labels in multi-layered protein films on indium tin oxide nanoparticle-based electrode

Facile electrical communication between redox-active labeling molecules and electrode is essential in the electrochemical detection of bio-affinity reactions. In this report, nanometer-sized indium tin oxide (ITO) particles were employed in the fabrication of porous thick film electrodes to enhance the otherwise impeded electrochemical activity of redox labels in multi-layered protein films, and to enable quantitative detection of avidin/biotin binding interaction. To carry out the affinity reaction, avidin immobilized on an ITO electrode was reacted with mouse IgG labeled with both biotin and ruthenium Tris-(2,2′-bipyridine) (Ru-bipy). The binding reaction between avidin and biotin was detected by the catalytic voltammetry of Ru-bipy in an oxalate-containing electrolyte. On sputtered ITO thin film electrode, although a single layer of Ru-bipy labeled avidin exhibited substantial anodic current, attaching the label to the outer IgG layer of the avidin/biotin-IgG binding pair resulted in almost complete loss of the signal. However, electrochemical current was recovered on ITO film electrodes prepared from nanometer-sized particles. The surface of the nanoparticle structured electrode was found by scanning electron microscopy to be very porous, and had twice as much surface binding capacity for avidin as the sputtered electrode. The results were rationalized by the assumption of different packing density of avidin inner layer on the two surfaces, and consequently different electron transfer distance between the electrode and Ru-bipy on the IgG outer layer. A linear relationship between electrochemical current and IgG concentration was obtained in the range of 40-4000 nmol L⁻¹ on the nanoparticle-based electrode. The approach can be employed in the electrochemical detection of immunoassays using non-enzymatic redox labels.     

大豆苷元与牛血清白蛋白的相互作用研究

采用荧光和紫外-可见吸收光谱,研究了大豆苷元与牛血清白蛋白(BSA)的相互作用.结果表明大豆苷元对BSA有较强的荧光猝灭作用;根据Stern-Volmer方程得到大豆苷元与BSA之间的结合常数KA为0.385×105 (30℃)、0.405×105(40℃)和0.431×105(50℃).根据F(o)rster非辐射能量转移理论,求出了大豆苷元与BSA之间的结合距离为2.34 nm(30℃)、2.48 nm(40℃)和2.71 nm(50℃).热力学数据表明大豆苷元与BSA之间的作用力主要为疏水作用力,同时用同步荧光光谱探讨了大豆苷元对BSA构象的影响.     

Influence of hydroxyapatite particle characteristics on the [K]/[Na] ratio: a human monocytes in vitro study

Research in bone substitutes has shown that calcium phosphates, particularly hydroxyapatite are well adapted to use in prosthetics. Due to its characteristics that are close to those of bone, hydroxyapatite is used as a coating or bone defect filler. At long term, one of causes of prosthetic failure is bone lysis followed by aseptic loosening. Particles released at the implantation site may be one cause of this phenomenon. It is important to address the loosening problem because longer implantation times are now required due to the increasing life expectancy of the population. To understand these effects and to optimise implantation of hydroxyapatite based prostesis, simulated wear debris produced by hydroxyapatite powders of various shapes, sizes and sintering temperatures were introduced into cultured human monocytes. Monocytes were chosen because they are among the first cells to colonise the inflammatory site. Cellular alteration caused by the particles is evaluated by measuring intracellular potassium to sodium ratios. Elemental intracellular ionic concentrations were measured in cells close to the physiological state by using cryomethods combined with electron probe microanalysis. This study demonstrated that various powder characteristics influence cellular viability. It was found that needle shaped particles are the most toxic whereas powders with particle size greater than 30 μm are the least toxic.