STRUCTURE IN THE PRECORE REGION OF HEPATITIS B CORE GENE AFFECTING ITS EXPRESSION IN E.coil

Restriction fragments of HBV-DNA, cleaved by endonuclease HhaI,containing HBcAg gene were trimmed by BAL-31 exonuclease to remove different lengths of the precore sequence.They were inserted into plasmid pUR222 at EcoRI site through synthetic linker ligation. Transformants in E.coli BMH7118 showing different levels of HBcAg gene expression were screened and analyzed for their nucleotide sequences in the junction region both by Maxam and Gilbert's chemical degradation method and by M13 chain termination method. Results of sequence analysis of different transformants revealed a partial palindromic (loop and stem) structure, at -7 to -35 nucleotide with regard to ATG of the HBcAg gene as position +1, which has dramatic effect on the level of expression of the inserted gene using the same promoter,SD sequence and identical N-terminus.The amount of HBcAg synthesized differed from 9% in the high expressing plasmid to less than 0.01% of the total cell proteins in the low expressing transformants.The findings w     

Stokes问题各向异性网格Q2-P1混合元超收敛分析

讨论Stokes问题在各向异性冈格下的Q2-P1混合有限元方法，利用积分恒等式技巧得到了与传统方法相同的超逼近性质，同时基于插值后处理的技巧，构造了速度和压力的一对插值后处理算子，并且前者具有备向异性特征，从而导出了整体超收敛结果．     

大型飞机结构整体优化设计

本文针对大型飞机结构整体优化设计中的难点,建立了适用于全机结构优化设计的组合算法。1)为了分析全机结构的需要,给出大型线性方程集的一种有效解法,使方程组带宽的大小几乎不受限制。2)发展具有分枝系统大型结构的组合算法,充分利用结构特性和有限元分析特点,减少计算工作量,节省计算时间和机器的存贮量。3)求大型规划问题时,由机器于内存容量不足及计算时间过长的矛盾,提出一种有约束最优化问题的大步梯度法。从而使大型飞机结构整体优化设计得以经济地完成。     

木糖腺苷类似物的合成

糖部分修饰的核苷类似物,作为有效的抗肿瘤及抗病毒药物,受到广泛的注意。但木糖系列则研究得较少。据报道木糖苷也显示抗肿瘤活性,木糖腺苷虽呈一定的抗肿瘤活性,但毒性较大。为了进一步研究木糖苷类似物的药理作用,我们合成了一系列8-取代的β-D-呋喃型木糖基腺嘌呤。     

么正最小模型的聚合与辫子矩阵的明显表示

本文利用Feigin-Fuch积分法明显地导出了么正最小模型的聚合和辫子矩阵,证明了D-F超方程的解是存在的.     

Eu~(2+)在xMF_2-yYF_3体系中光谱性质及其变化规律

合成了Eu~(2+)激活的碱土稀土复合氟化物体系xMF_2-yYF_3(x=1,2,3;y=0,1,2,3,4,5;M=Ca,Sr,Ba),系统地研究了Eu~(2+)在该体系中的光谱性质及其变化规律,讨论了影响Eu~(2+)光谱的因素。     

A simple encapsulation method for organic optoelectronic devices

The performances of organic optoelectronic devices, such as organic light emitting diodes and polymer solar cells,have rapidly improved in the past decade. The stability of an organic optoelectronic device has become a key problem for further development. In this paper, we report one simple encapsulation method for organic optoelectronic devices with a parafilm, based on ternary polymer solar cells(PSCs). The power conversion efficiencies(PCE) of PSCs with and without encapsulation decrease from 2.93% to 2.17% and from 2.87% to 1.16% after 168-hours of degradation under an ambient environment, respectively. The stability of PSCs could be enhanced by encapsulation with a parafilm. The encapsulation method is a competitive choice for organic optoelectronic devices, owing to its low cost and compatibility with flexible devices.     

小隔板试验冲击起爆和熄灭流场的实验与Lagrange分析

根据小隔板试验的拉氏传感器测试结果,用拉氏分析方法求得了粗颗粒包复TNT炸药中的冲击波成长过程和熄灭过程的流场分布,用实验结果确定了点火增长模型中的待定参数。利用拉氏分析方法时,对未反应炸药和已反应产物的状态方程都采用JWL状态方程,使确定的参数可以直接用于有限元程序LSDYAN计算。通过拉氏分析得到了各迹线上的反应度,结果表明,冲击波在熄灭过程中仍能引起炸药的局部化学反应。