共查询到20条相似文献,搜索用时 140 毫秒
1.
2.
稳健灰色模型在色谱保留值研究中的应用 总被引:6,自引:2,他引:6
本文提出了具有稳健性的GM(1,1)灰色模型,并用此模型对8种多环芳烃化合物容量因子与流动相组成间的关系作了研究,建立了其间的稳数学模型,结果表明,稳健GM(1,1)模型比常规GM(1,1)模型具有更好的抗干扰性能和受异常点影响小的优点,是一个值得推广的好方法。既丰富了灰色系统理论,又拓宽了灰色理论在分析化学中的应用范围。 相似文献
3.
连续型非等时空距灰色模型在液相色谱保留值研究中的应用 总被引:12,自引:2,他引:12
本运用灰色理论中的连续型等时空距GM(1,1)模型,对液相色谱中流动相组成与容量因子间的关系进行了研究,建立了可进行连续预测的数学模型,所建模型与实际情况吻合很好,经检验各模型精度均达到I级。 相似文献
4.
非等时空距灰色模型在液相色谱保留值研究中的应用 总被引:8,自引:3,他引:8
本文运用灰色理论中的非等时空距GM(1,1)模型,对液相色谱中的流动相组成及容量因子的关系进行了研究。所建灰色模型经过检验其精度为I级。实验结果表明,不仅等时空距灰色模型,而且非等时空距灰色模型,同样适用于色谱保留值的研究。 相似文献
5.
色谱灰色数模的建立及其应用研究(Ⅱ)——NLCGM(1,1)模型的建立及芳烃保留行为的预测 总被引:1,自引:0,他引:1
色谱保留值与流动相组成间关系的研究一直是色谱工作者关心的课题[1,2].灰色模型对于处理具有不确定关系的两因素间的关系问题具有独到之处,在容量因子(k)与流动相组成(φ)关系的研究方面已得到了应用[3~8].传统GM(1,1)模型将k与φ之间的关... 相似文献
6.
应用灰色系统理论和最小二乘原理,推导出连续GM(1,N)模型,并将该模型用于高效液相色谱中溶质保留行为的预测.研究了烷烃、烷基苯及对羟基苯甲酸酯等化合物的容量因子与流动相组成、容量因子与碳数、保留时间与温度、保留时间与碳数的关系,并建立了相应的数学模型,经验证,预测结果良好. 相似文献
7.
本文从麦克雷诺(McReynolds)相常数法出发推导了五种标准物质与任意溶质i的色谱保留指数之间的关系符合灰色GM(0,6)模型,对它们进行了灰色建模。获得了很高的模型精度,并根据模型式预测了涂有不同固定液色谱柱中的溶质i的保留指数。通过预测值与文献值的比较,得到了良好的预测精度。 相似文献
8.
9.
熊果酸与齐墩果酸高效液相色谱分离条件优化 总被引:2,自引:0,他引:2
描述了熊果酸、齐墩果酸色谱保留行为与流动相pH和有机溶剂体积分数之间的函数关系,并求解了各自模型中的9个参数,得到在流动相中熊果酸的电离常数pKa=4.8145,齐墩果酸电离常数pKa=4.9317。文章还对模型外推过程中存在的误差进行了校正,并运用校正后的模型预测熊果酸的容量因子,实验值接近预测值,误差小于5%。最后采用熊果酸和齐墩果酸的容量因子与流动相pH和有机溶剂组成之间的函数关系,进行了两者在同一流动相中分离条件的优化,获得了最佳的分离条件:流动相中甲醇体积分数为85.2%,pH为6.50。 相似文献
10.
11.
An effective method for the synthesis of 1-(cyclopentadienyl)adamantane based on the reaction of 1-bromoadamantane with nickelocene
in the presence of PPh3 was proposed. A number of organic derivatives and bis-cyclopentadienyl complexes of zirconium(iv) were prepared from this compound.
Translated fromIzvestiya Akademii Nauk. Seriya Khimicheskaya. No. 3, pp. 506–510, March, 2000. 相似文献
12.
Hypoxia-inducible factor-1 (HIF-1) is among the most important indicators of hypoxia in evaluating severity of many diseases. In this work, a novel method for HIF-1 detection is proposed by using electrochemical techniques based on small molecule binding DNA. In this method, since the designed DNA sequence can specifically bind with either an electroactive small molecule or HIF-1, the signal readout is inversely proportional to HIF-1 concentration, thus a simple and easily-operated method for HIF-1 detection can be developed. With the proposed method, HIF-1 can be determined in a linear range from 5 to 25 nM with a detection limit of 2.8 nM. Furthermore, the proposed method can be directly used to assay HIF-1 in placenta tissue, and the assay results can reliably reflect the severity of preeclampsia, a very dangerous condition during pregnancy. The proposed method also shows desirable sensitivity, high selectivity and excellent reproducibility, so this method can have potential applications in clinical practice. 相似文献
13.
《Analytical letters》2012,45(16):2324-2333
A new, sensitive, and simple HPLC method was described for the determination of 1-aminocyclopropane-1-carboxylic acid (ACC) in apple and peach extracts. The method was based on the derivatization of ACC with fluorescamine in borate buffer systems of pH 8.0 to yield a highly fluorescent product. The experimental parameters affecting the derivatization reaction efficiency were optimized by fluorimetric analysis. Under optimum derivatization conditions, the derivative product of ACC in apple and peach extracts without extra purification was successfully chromatographed on a C-18 column by HPLC coupled to fluorescence detection. The derivative product of ACC with fluorescamine could be well separated from other concomitant substances or their derivatives that might interfere with the determination of ACC. The linearity of ACC was measured in the range of 23.82–238.82 µg · L?1 with a good correlation coefficient of 0.9997. Based on signal-to-noise ratio of 3, a low detection limit of 5.0 µg · L?1 could be reached. The proposed method was applied successfully to the determination of ACC in the crude apple and peach extracts without extra purification with low RSDs of 0.19–1.9% and good recoveries of 90.89–104.4%. The sensitive HPLC quantitative method is of great significance for the investigations of ACC metabolism in plants. 相似文献
14.
单扫描极谱法测定注射液及血清中维生素K1 总被引:5,自引:0,他引:5
建立了维生素K1的极谱测定方法.在0.05 mol·L-1 NH4Cl-NH3(pH 8.58)缓冲溶液中,维生素K1可产生一极谱还原峰,峰电位Ep为-0.39 V ;其二阶微分峰峰电流ip″与维生素K1的浓度在1.1×10-7 ~2.2×10-5 mol·L-1范围内呈线性关系,相关系数r=0.998 6(n= 9 );方法的检出限为4.0×10-8 mol·L-1.13次平行测定2.2×10-5 mol·L-1维生素K1还原波二阶导数峰峰电流ip″的相对标准偏差(RSD)为1.83%.本法可直接用于注射液及血清中维生素K1含量的测定. 相似文献
15.
V.E. Kuz’min A.G. Artemenko S.Yu. Makan S.A. Andronati 《SAR and QSAR in environmental research》2013,24(3-4):213-244
The influence of molecular structure of 346 ligands on their affinity for 5-HT1A receptors was investigated. It was shown that the effectiveness of the proposed novel approach for interpretation of decision tree models compared favourably with the PLS method. In the context of the proposed approach, molecular fragments and their values of the relative influence on the affinity for 5-HT1A receptors were defined. 相似文献
16.
《Analytical letters》2012,45(18):2961-2973
AbstractA novel enzyme-linked aptamer assay is reported for the determination of aflatoxin B1 (AFB1). AFB1 can competitively bind with the immobilized biotin-aptamer and release biotin complementary DNA, leading to the gradual fading of the detection system color with increasing of AFB1 concentration. In the absence of AFB1, the biotinylated complementary DNA is not be released from the fixed aptamer. Therefore, the enzyme reaction occurs in the detection system. Under the optimized experimental conditions, the proposed method possessed a wide linear range for AFB1 from 1 to 80?ng/mL (R2 of 0.990) with a low detection limit of 0.36?ng/mL. The method was then applied to detect uncontaminated peanuts fortified with different concentrations of AFB1. The recovery values were from 82.60% to 94.43%, which indicated the proposed method may be used to detect AFB1 in food and has potential for the development of test kits. 相似文献
17.
18.
19.
20.
Lichitsky B. V. Kozhinov D. V. Vorontsova L. G. Starikova Z. A. Dudinov A. A. Krayushkin M. M. 《Russian Chemical Bulletin》2002,51(10):1869-1874
A general and convenient method for the synthesis of 12-aryl-1-oxo-1,2,3,4,5,12-hexahydroindolo[2,1-b]quinazoline-6-carbonitriles was proposed. The method is based on the recyclization of 11-aryl-1-oxo-2,3,4,5,10b,11-hexahydro-1H-indolo[2,3-b]quinoline-10b-carbonitriles. The structure of 12-(3-methoxyphenyl)-8-methyl-1-oxo-1,2,3,4,5,12-hexahydroindolo[2,1-b]quinazoline-6-carbonitrile was studied by X-ray diffraction analysis. 相似文献