细胞色素C在聚乙烯氧化物修饰的金电极上的直接电化学反应

本文报道了细胞色素Ｃ在聚乙烯氧化物修饰的金电极上的直接电化学行为，发现ＰＥＯ是细胞色素Ｃ电化学反应的促进剂，ＰＥＯ修饰膜的形态对细胞色素Ｃ电化学反应的可逆性有较大的影响。     

细胞色素C在吡啶,聚吡啶修饰的金电极上的直接电化学

研究了细胞色素Ｃ在吡啶修饰的金电极上的电化学反应，结果表明，只具有一个功能团的吡啶分子和它的聚合物对细胞色素Ｃ电化学反应也有促进作用，讨论了影响促进剂促进作用的主要因素。     

修饰微带金电极上细胞色素C电极过程的热力学研究

研究了细胞色素Ｃ（Ｃｙｔ．Ｃ）在一种新的电子转移促进剂４，６－二甲基－２－巯基嘧啶修饰微带金电极（ＤＭＭＰ／Ａｕ）上的氧化还原热力学；求得Ｃｙｔ．Ｃ在ＤＭＭＰ／Ａｕ电极上反应的标准电极电位Ｅ＾ｏ，熵变△Ｓ＾ｏ，焓变△ＩＩ＾ｏ及Ｇｉｂｂｓ自由能的改变△Ｇ１０值分别为：０．２７２Ｖ（ｕｓ．ＮＨＥ），－１２３．７Ｊ．ｍｏｌ＾－＾１，－６３．１ｋＪ．ｍｏｌ．ｍｏｌ＾－＾１和－２６．２ｋＪ．ｍｏｌ＾－＾１（     

十六烷基三辛基铵静电吸附氯金酸的Langmiur—Blodgett膜及其修饰…

首次尝试用Ｌａｎｇｍｉｕｒ－Ｂｌｏｄｇｅｔｔ（ＬＢ）技术修饰ＰＶＣ液膜离子选择性电极，在ＰＶＣ液膜表面上制得十六烷基三辛基铵－氯金酸ＬＢ膜，明显改善了ＰＶＣ液膜金离子选择性电极的某些工作性能。电极线性响应范围为１×１０＾－２～１×１０＾－７ｍｏｌ／Ｌ，对常见的９种阳离子和４种阴离子的干扰能力明显的增强，响应速度也有所提高。若能改进挂膜方式，可望进一步改善有关性能。     

光化学催化—示波电位法测定维生素B2

利用维生素Ｂ２被ＥＤＴＡ光致还原产物ＶＢ２的１，５－二氢化物，被碘酸钾氧化为ＶＢ２的光催化过程，以示波电位法测定电极电位的变化率，建立△Ｅ－ＣＶＢ２，１／ｔ－ＣＶＢ２线性关系。提出光电联合测定ＶＢ２的固定电位，固定时间分析方法，准确，快速。     

细胞色素c在L-半胱氨酸自组装膜电极上的电化学行为

细胞色素c在L-半胱氨酸自组装膜电极上的电化学行为;细胞色素c; L-半胱氨酸; H2O2; 金电极     

电沉积制备ZnO纳米棒修饰电极上氧化还原蛋白的电化学行为

采用恒电位阴极还原法在金电极表面一步修饰ZnO纳米棒, 制备成ZnO纳米棒修饰电极. 扫描电子显微镜(SEM)和X射线衍射(XRD)结果显示制得的ZnO为直径约100 nm的六棱柱状纤锌矿晶体纳米棒. 使用ZnO纳米棒修饰的金电极研究细胞色素c的直接电化学行为, 结果表明: ZnO纳米棒修饰的金电极能有效探测到细胞色素c的铁卟啉辅基在不同价态下的电化学行为; 细胞色素c吸附后, ZnO纳米棒修饰的金电极对过氧化氢的电流响应呈现良好的线性关系.     

导数—同步荧光光谱法同时测定三种B族维生素的研究

研究了维生素Ｂ１的氧化产物（硫色素）、维生素Ｂ２和Ｂ６混合物体系的导数－同步荧光光度谱，提出了混合物体系荧光光谱被分辨开的同时测定方法，该法可不经分离直接测定复合维生素片剂中维生素Ｂ１，Ｂ２及Ｂ６，其线性范围均为０－１．０μｇ／ｍＬ，检出限分别为０．５、１．５及４．０ｎｇ／ｍＬ。     

吡啶、2-甲基吡啶存在下细胞色素c碱式异构化和配体细胞色素c配合物的电化学研究

用半胱氨酸修饰的金电极研究了吡啶、2 甲基吡啶存在下细胞色素c碱式异构化和配体结合细胞色素c的电化学。在此电极上 ,细胞色素c可发生准可逆的电极反应而吡啶结合细胞色素c和 2 甲基吡啶结合细胞色素c在循环伏安图上只给出还原峰。高浓度 (1.2 7mol·L- 1)的吡啶和 2 甲基吡啶可诱导碱式细胞色素c在中性条件下生成。进一步的研究表明 ,这种诱导作用与配体和细胞色素c的键合无关     

自模式分辨红外光谱法在VC,VB2不分离测定中的应用

本文研究了自模式曲线分辨红外光谱法在维生素Ｃ－Ｂ２混合物体系中的应用，合理地估计了背景吸收。测定了不同配比维生素Ｃ－Ｂ２混合物组分，平均回收率分别为维生素Ｃ１０１．７±４．４％、维生素Ｂ２９９．４±３．５％，优于Ｂｅｃｋｍａｎ公司的标准曲线法：维生素Ｃ９３．０±２１．１％、维生素Ｂ２１０４．３±１５．２％。     

细胞色素C在吡啶、聚吡啶修饰的金电极上的直接电化学

研究了细胞色素C在吡啶修饰的金电极上的电化学反应,结果表明,只具有一个功能团的吡啶分子和它的聚合物对细胞色素C电化学反应也有促进作用,讨论了影响促进剂促进作用的主要因素。     

细胞色素C在糖及醇修饰金电极上的直接电化学

细胞色素C在生物体内能进行可逆的氧化还原反应，但它知不能直接在金属电极上进行可逆的电化学反应．因此在早期细胞色素（：的电化学研究中，人们往往借助干一类称为媒介体山的分子，这给细胞色素C的电化学反应研究带来很大的不便．IO77年，hill等人间发现在4／一联毗陡存在下     

Reduction of cytochrome c at a lipid bilayer modified electrode

The reduction of horse heart cytochrome c has been investigated at a platinum electrode modified with a lipid bilayer membrane (BLM) which immobilized vinyl ferrocene as an electron mediator. The current—voltage curves show that the direct electrochemistry of cytochrome c at the metal electrode occurs quite efficiently. An adsorption equilibrium constant for cytochrome at the BLM surface, as well as an electron transfer rate constant between the protein and the modified electrode have been estimated from these results. The values of both parameters are much higher than those reported with other types of electrode modifications, indicating that a lipid bilayer-modified platinum electrode system using vinyl ferrocene as a mediator provides substantial improvements in electrochemical activity of cytochrome c at metal electrodes. The potential for modifying and utilizing this new class of “biomembrane-like” electrode surface for metalloprotein electrochemistry is briefly discussed.     

Surface modifiers for the promotion of direct electrochemistry of cytochrome c

Fifty-four bifunctional organic compounds were studied to assess their ability to promote the direct electrochemistry of horse heart cytochrome c at a modified gold electrode. From the results of the survey it was possible to identify those features important for successful promotion of the electrochemical activity. It is suggested that it is necessary to provide groups on the electrode surface which can hydrogen bond or form salt bridges to the positively charged lysine side chain groups around the heme crevice on cytochrome c. The functional groups, Y, may be anionic or weakly basic and can be attached to the electrode using a bifunctional compound X ～ Y. The group, X, adsorbs or binds to the gold surface through nitrogen, phosphorus, or sulphur. A “pre-activation” step for the adsorption of some surface modifiers has been discovered. The molecular structure of a compound which promotes cytochrome c electrochemistry can be either conformationally rigid or flexible, aromatic or aliphatic, but it should direct Y out from the electrode. The length of the molecule does not appear to affect the rate of electron transfer. The presence of a hydrophobic zone in the structure is neither necessary nor sufficient for successful promotion of cytochrome c electrochemistry.     

细胞色素C551在ITO电极上的直接电化学

在不加任何电子转移促进剂的条件下,用循环伏安法(CV)和微分脉冲伏安法(DPV)观察到细胞色素C551在ITO导电玻璃上的直接电化学行为.结果显示电极反应过程为准可逆性质,计算得到细胞色素C551的扩散系数、式电位和异相电子转移标准速率常数,并对其在ITO导电玻璃电极上的电子转移机制进行了初步分析.     

Electrochemical and ultraviolet-visible spectroscopic studies on the interaction of deoxyribonucleic acid with vitamin B6

Studies on the interaction of DNA with vitamin B(6) were carried out with a DNA-modified electrode by electrochemistry and Ultraviolet-visible spectroscopy. The results showed that there exists the supra-molecule interaction between base groups on DNA and vitamin B(6) by forming hydrogen binding, the binding equilibrium constant of the interaction is equal to 115.3 M(-1), the binding ratio of nucleotide to vitamin B(6) is 5:1. Based on the electrochemical and Ultraviolet-visible spectrum studies the interaction mode of DNA with vitamin B(6) was explored.     

支持向量回归同时测定维生素B混合物的研究

用支持向量回归方法(SVR)建立校正模型,并对维生素B1、B2和B6的模拟样品进行测定。结果表明,维生素B1、B2和B6预测结果的回收率在96%~104%之间,测定结果准确。     

Direct Electrochemistry of Cytochrome c at Gold Electrode Modified with Fumed Silica

Direct electrochemistry of horse heart cytochrome c (cytc) has been obtained at a gold electrode constructed by self‐assembling fumed silica particles (FSPs) onto a silane monolayer. A pair of well‐defined and nearly symmetrical redox peaks of cytc is obtained at the FSPs film modified gold electrode. Cyclic voltammetry (CV) and tapping‐mode atomic force microscopy (AFM) are used to characterize the FSPs film modified electrode, showing that the FSPs can provide a favorable microenvironment for cytc and facilitate the direct electron transfer between the cytc and the gold electrode, which may propose an approach to realize the direct electrochemistry of other proteins.     

DNA与Rt、VB2相互作用的电化学性质

芦丁(Rt)是存在于多种植物体中的一种多羟基黄酮化合物,是某些中草药的有效成分。本文在前文基础上着重对芦丁、VB2在玻碳电极上的电化学性质、二者与DNA相互作用的机理等方面的差异进行比较,这对维生素类药物与生物大分子相互作用的研究与应用具有重要意义。     

The Electrochemical Properties of Polyalizarin Violet Modified Electrode and its Electrocatalytic Oxidation of Ascorbic Acid

Ascorbic acid is a vital vitamin in the diet of human and is present in mammalian brain. However, it is always too difficult to determine this compound electrochemically by direct oxidation at a conventional electrode because of the fouling by its oxidation products. In order to resolve this problem, a multitude of polymeric coating for a wide variety of electrode applications in electrochemistry have been purposed In this paper, we reported the fabrication of polyalizarine violet chemical modified electrode prepared by electropolymerization directly at a glassy carbon electrode and its use in catalytic oxidation of AA.