玻璃微-纳流控芯片的制备及在蛋白质电动富集中的应用

发展了一种以"二次刻蚀"技术制备玻璃微-纳流控芯片的新方法. 首先, 采用紫外光刻和化学湿法刻蚀技术在玻璃基片上加工微米深度的微通道; 去除剩余的光胶后, 在刻有微通道的基片上旋涂一层新的光胶; 再通过二次紫外光刻和湿法刻蚀在该基片上加工深度小于100 nm的纳通道; 最后, 采用室温键合技术, 将带有微纳结构的基片与盖片封合制成玻璃微-纳流控复合芯片. 利用本方法可以在普通化学实验室以简易的设备制得具有微-纳米复合结构的玻璃芯片. 将此玻璃微-纳流控复合芯片成功地应用于以电动离子捕集技术富集荧光素钠异硫氰酸酯(FITC)标记的人血清蛋白(HSA). 结果表明, 对于0.5 mg/mL的FITC-HSA, 30 s内富集倍率可达到200倍以上.     

玻璃微流控芯片廉价快速制作方法的研究

研究了一种玻璃微流控芯片的快速、低成本制作工艺和方法. 该方法采用商品化的显微载玻片(soda-lime玻璃)作为芯片基质材料, 利用AZ 4620光刻胶代替传统工艺中的溅射金属层或多晶硅/氮化硅层作为玻璃刻蚀的掩膜层, 同时利用一种紫外光学胶键合方法代替传统熔融键合方法实现芯片的键合, 整个工艺对玻璃基质材料要求低, 普通微流控芯片(深度小于50 μm)制作流程仅需约3.5 h, 可降低制作成本, 缩短制作周期. 还系统地研究了光刻胶厚度、光刻胶硬烘时间和玻璃腐蚀液配比对玻璃微流控芯片制作的影响, 获得了优化的工艺参数.     

注塑型聚甲基丙烯酸甲酯多通道微流控芯片的研制及其性能考察

微流控芯片技术因具有微量、快速、高效和高通量等特点,已成为分析化学领域中的研究热点之一．在微流控芯片中,最常见的可用作芯片的材料为玻璃、石英和各种塑料．玻璃和石英有很好的电渗性和光学性质,可采用标准的刻蚀工艺加工和用化学方法进行表面改性,但加工成本较高,封接难度较大．     

纸基过氯乙烯树脂微流控亚硝酸根离子检测片的研制

基于滤纸上过氯乙烯树脂栏选择性通过亚硝酸根离子, 结合微流控分析装置设计, 研制成纸基过氯乙烯树脂微流控亚硝酸根离子检测芯片. 采用该微流控亚硝酸根离子检测芯片测定了亚硝酸盐样品, 线性范围和检测限分别为70～1500 μmol/L 和48 μmol/L. 该微流控亚硝酸根离子检测芯片已成功应用于水样和食品中亚硝酸盐测定, 结果满意.     

微流控芯片-激光诱导荧光检测器的研制及核酸片段分离检测中应用

采用自行设计的共焦式激光诱导荧光检测器（激发波长635nm，发射波长670nm），以cy5染料对检测器的空间分辨能力和灵敏度进行了测试，检出限达到10^-9mol／L。建立了一种微流控芯片快速分离检测DNA片段的方法。以0．8％羟丙基甲基纤维素（HPMC）为筛分介质，以（噻唑橙单体）To-pro-3为荧光标记染料，在玻璃微流控芯片中实现了dsDNA片段的无胶筛分和激光诱导荧光检测，12条DNA片段在75s内得到分离。     

聚二甲基硅氧烷/玻璃微流控芯片永久性粘合方法及其微通道表面改性研究及其应用

近年来,聚二甲基桂氧烷[Poly(dimethylsilloxane),PDMS]基质微流控芯片因其透光性能好,价格便宜,加工容易,适合大规模生产,成为微全分析系统(Micro total analysis system,μ-TAS)发展的一个热点^[1].PDMS易于复制微通道形状,且具有较高的保真度,省去了玻璃芯片刻蚀的复杂过程;而玻璃具有易于集成功能单元,散热性能好的优点,PDMS-玻璃杂合微流控芯片同时结合了PDMS和玻璃的优点,具有良好的发展前景^[2].     

聚甲基丙烯酸甲酯微流控芯片分离DNA片段的初步研究

自从1995年Mathies^[1]首次将微流控芯片毛细管凝胶电泳用于基因测序研究以来,DNA片段的分离已成为微流控芯片应用的重要领域之一.最早应用于DNA分析的微流控芯片是玻璃芯片,聚合物微流控芯片以其品种多、成本低、易于加工,与玻璃芯片相比具有封接温度大大降低,微通道内电渗流显著减小等特点,已被成功应用于DNA片段的分离^[2,3].     

自制玻璃微流控芯片及其基本性能考察

20世纪90年代初,自Manz等首次提出微全分析系统的概念以来,各种不同材料和功能的微流控芯片不断问世,其中应用得最多的是玻璃、石英及聚合物3种材料．玻璃和石英芯片因具有优异的电渗、光学和表面性质,其刻蚀加工技术和表面改性的化学方法均比较成熟,且传统毛细管电泳中各种成熟的分离方法可直接应用到玻璃芯片的制作中,因此在微全分析系统研究中具有十分重要的地位．     

微流控芯片化学发光检测系统的设计及其应用

通过标准光刻、化学刻蚀及热键合技术制作微流控电泳芯片,在芯片上集成流通式化学发光检测池,实现样品的芯片电泳分离化学发光检测.采用双(2,4,6-三氣苯基)草酸酯(TCPO)-过氧化氢化学发光体系,通过微泵输送化学发光试剂.单酰化苯并氨基酸和单酰化肌氨酸在该系统中得以成功地分离检测,其检测限分别达到2.8和3.2 μmol/L.     

回形针固定法制作三维(3D)微流控纸芯片

提出一种利用回形针来制作三维微流控纸芯片的方法,能够适应多种不同的微流控纸芯片;利用自制的纸芯片比色检测装置,已用于牛血清白蛋白,Fe~(2+)的快速定量检测。牛血清白蛋白的线性范围是5~50μmol/L,检出限为0.13μmol/L,R~2=0.994。Fe~(2+)的线性范围是0.6~12μmol/L,检出限为0.15μmol/L,R~2=0.992。在所规定的范围内,都获得了较好的线性关系,验证了基于回形针的3D微流控纸芯片可用于实际样品检测的可能性。     

α-Amino acid derived enaminones and their application in the synthesis of N-protected methyl 5-substituted-4-hydroxypyrrole-3-carboxylates and other heterocycles

A new and simple synthesis of novel N-protected methyl 5-substituted-4-hydroxypyrrole-3-carboxylates, which exist in equilibrium with their 4-oxo tautomers, has been developed in two steps starting from N-protected α-amino acids. The key intermediates are enaminones, which can also be isolated, characterized, and used for the construction of other functionalized heterocycles, before they spontaneously decompose to pyrrole products. 4-Hydroxypyrroles are prone to partial aerial oxidation but can be efficiently alkylated or reduced to stable polysubstituted pyrrolidine derivatives.     

Specific intramolecular aromatic CH insertion of diazosulfonamides

The chemoselectivity in the intramolecular CH insertion of various diazosulfonamides has been experimentally studied. The results reveal that the aliphatic 1,4-, 1,5-, or 1,6-C(sp³)?H insertions of diazosulfonamides are not accessible, while the aromatic 1,5-C(sp²)?H insertion can be realized specifically by adjusting the diazo-adjacent group. In addition, the general chemoselectivities in the intramolecular CH insertions of diazosulfonyl compounds are summarized. Generally, diazosulfones undergo both aromatic 1,5-C(sp²)?H and aliphatic 1,5- and 1,6-C(sp³)?H insertions, while diazosulfonates undergo aliphatic 1,5- and 1,6-C(sp³)?H insertions. However, diazosulfonamides only undergo aromatic 1,5-C(sp²)?H insertion.     

N-Heterocyclic carbene-palladacyclic complexes: synthesis,characterization and their applications in the C-N coupling and α-arylation of ketones using aryl chlorides

N-Heterocyclic carbene-palladacyclic complexes 3 were successfully achieved in a one-pot procedure under mild conditions. The structure of 3a was unambiguously confirmed by X-ray single crystal diffraction and it was an active catalyst in the Buchwald-Hartwig amination and α-arylation of ketones even at very low catalyst loadings (0.01?mol%).     

Iodine-mediated oxidative Pictet-Spengler reaction using terminal alkyne as the 2-oxoaldehyde surrogate for the synthesis of 1-aroyl-β-carbolines and fused-nitrogen heterocycles

An efficient iodine-mediated oxidative Pictet-Spengler reaction in dimethyl sulphoxide (DMSO) using terminal alkynes as the 2-oxoaldehyde surrogate for the synthesis of aryl (9H-pyrido[3,4-b]indol-1-yl)methanones is described. The scope of the protocol includes the total synthesis of Fascaplysin, Eudistomins Y₁ and Y₂. The methodology is extended for preparing pyrrolo[1,2-a]-quinoxaline and indolo[1,5-a]quinoxaline derivatives. The utility of 1-aroyl-β-carbolines was demonstrated by performing palladium-catalyzed β-carboline directed ortho-C(sp2)-H functionalization of the phenyl ring with thiomethyl (SMe) group using DMSO as source and for accessing 4-aryl-canthin-6-ones.     

Facile synthesis of bicyclic 1-arylpyrazol-5-ones

In this Letter, we described a facile method for constructing fused bicyclic 1-arylpyrazol-5-one ring system. We employed various methylene-containing carboxylic acids as the substrates and proved that the pyrazolone ring closure requires activated methylene group in intermediate II. Accordingly, a series of structurally diversified, fused bicyclic 1-arylpyrazol-5-ones was prepared in moderate to high yields using the requisite substrates.     

Synthesis of pyrrolo[1,2-a]pyrazines and pyrazino[1,2-a]indoles by Curtius reaction in Morita-Baylis-Hillman derivatives

Synthesis of substituted pyrrolo[1,2-a]pyrazines and pyrazino[1,2-a]indoles from the Morita-Baylis-Hillman derivatives of acrylates via saponification followed by Curtius reaction is described.     

正丁胺对激光染料荧光谱影响的研究

用正丁胺作为碳源,采用射频辉光放电制备碳膜,选用激光染料R6G和聚乙二醇混合液作为蒸气源,采用单源热蒸发,在蒸发室与染料同时沉积得到混合膜,用拉曼光谱和红外光谱分析了碳膜的结构和键合方式,分析表明:碳膜中存在胺基团和氢原子.混合膜的荧光谱测量结果表明,认为正丁胺对染料荧光谱的影响是因为胺基和氢原子的存在.     

Cu0-catalysed 1,3-dipolar cycloadditions of α-amino acid derived N,N-cyclic azomethine imines to ynones

A series of 20 CuAIAC reactions between eight 4-acylamino substituted pyrazolidine-3-one-1-azomethine imines and four terminal ynones were performed using Cu⁰ as catalyst. The corresponding fluorescent cycloadducts were obtained in very high yields upon simple workup. Thus, Cu-metal turned out to be a better catalyst than Cu^I in terms of yield and ease of isolation. Availability of azomethine imines, mild reaction conditions, and simple workup enable a “click” access to libraries of densely substituted 2,3-dihydro-1H,5H-pyrazolo[1,2-a]pyrazol-1-ones. Reactivity of differently substituted dipoles was evaluated experimentally and by quantum chemical methods (DFT).     

Synthesis and antioxidative/anti-inflammatory activity of novel fullerene–polyamine conjugates

(E)-4-(Fullerenopyrrolidin-1-yl)-3-methylbut-2-enoic acid and its corresponding succinimidyl ester, readily obtained through Prato-type modification of C₆₀, were used for the selective N-acylation of polyamines. The thus obtained conjugates were evaluated for their antioxidative and anti-inflammatory activity and their cytotoxicity was determined. Members of this family of compounds showed interesting anti-lipid peroxidation, anti-lipoxygenase and anti-inflammatory activity and comparable cytocompatibility to spermidine.