共查询到19条相似文献,搜索用时 375 毫秒
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化学衍生法应用于生物多胺的HPLC荧光检测 总被引:1,自引:0,他引:1
介绍了在高效液相色谱荧光衍生法分析多胺中常用的几类荧光衍生试剂及其应用,对近年多胺分析中的高效液相色谱荧光衍生方法进行了综述。 相似文献
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聚乙二醇支持的底物或试剂参与的小分子化合物的合成 总被引:1,自引:0,他引:1
对近年来聚乙二醇为可溶性聚合物支持体支持的底物或试剂参与的小分子化合物的液相有机合成反应进行了总结。参考文献36篇。 相似文献
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反相高效液相色谱法同时测定食品中甜蜜素、糖精钠和苯甲酸钠 总被引:9,自引:0,他引:9
采用反相高效液相色谱法快速分离和测定食品中甜蜜素、糖精钠和苯甲酸钠,在ODS柱上,以30%甲醇和70%(含离子对试剂)水溶液为流动相进行分离;分别考察了流动相中不同的甲醇配比和不同浓度的离子对试剂对分离和保留行为的影响。检测波长为205nm。采用外标法定量。该方法精密度高、准确度好,样品处理简单,实用于常规质量监测。 相似文献
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离子对高效液相色谱—TPPS4光度法测定痕量锌和铜 总被引:1,自引:1,他引:1
本文用TPPS4作柱前衍生试剂,研究了试剂与锌和铜的配位反应,在ODS柱上,用乙腈/水体系作流动相,苄基三乙基氯化铵作离子对试剂,在415nm处检测,提出了离子对高效液相色谱-光度法快速分离测定痕量锌铜的新方法,检测限:Zn和Cu分别为0.15和0.10ng/mL,本法用于花生样品中痕量Zn,Cu的测定。结果与AAS一致。 相似文献
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柱前手性衍生色谱法拆分DL—氨基酸时流动相的影响 总被引:2,自引:0,他引:2
本文利用邻苯二甲醛和N-乙酰-L-半胱氨酸作柱前手性衍生化试剂,经反相高效液相色谱法拆分DL-氨基酸对映体,研究了流动相组成对出峰顺序和分离效果的影响。 相似文献
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A new ionic, water‐soluble scavenger for acyl chlorides, 1‐(2‐aminoethyl)pyridinium bromide ( 1 ), has been investigated. Compound 1 was used for the rapid and simple purification of a series of benzamides and sulfonamides (Table) obtained by solution‐phase synthesis from the corresponding amines (Scheme). The inexpensive scavenger, which can be prepared on large scale, was shown to readily ‘eliminate’ excess acyl chlorides (reagent) by simple aqueous extraction. The amides purified in this way were obtained in excellent yields and purities (Table), which makes 1 a versatile new reagent, especially for the combinatorial solution‐phase synthesis of amide libraries. 相似文献
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综述了近年来液相组合合成的研究进展,主要包括液相组合合成法(LPCS),氟合成,树状载体组合合成及高分子辅助试剂在液相组合合成中的应用等。 相似文献
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《Journal of fluorine chemistry》2006,127(4-5):565-570
We describe here the use of polyfluorinated trivalent iodonium salts as efficient and robust capping reagents during solid phase peptide synthesis using either t-Boc or Fmoc chemistry. Standard protocols established for solid phase peptide synthesis can be utilized without any change in solvent or reagent conditions. The capping reaction was carried out at sites where amino acid coupling steps failed. At the termination of the synthesis, the crude peptide mixture obtained from release of materials from the resin, is either simply centrifuged in aqueous solution to yield pure peptide, or purified by passage through fluorous silica gel in solvents with high water content. We envision that the chemistry and reagents described here will find wide use in peptide and protein chemistry, and also in combinatorial library synthesis where terminal amines are coupled to reaction partners. 相似文献
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随着组合化学的迅速发展, 兼容固相合成和溶液相合成优点的液相合成方法已成为实现组合化学的一条重要途径. 综述了近年来聚乙二醇为可溶性聚合物载体支载的杂环化合物库的研究, 并展望了其今后的发展方向. 相似文献
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A new method of purification of solution-phase combinatorial libraries has been developed. Development of a chemically inert polyaromatic anchor with a reactive "scavenger reagent" (PAHSR) allows unreacted reagents and impurities to be removed from a reaction by absorption of the PAHSR to charcoal and simple filtration. 相似文献
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The synthesis of three bipyridyl-tagged reagents and one scavenger is described. Of the three reagents, the carbodiimide derivative proved to be effective as a coupling reagent for amide formation and the removal of the coupling side product from the reaction mixture by complexation onto a Cu-derivatised resin has been successfully demonstrated. This purification process was thoroughly optimised using a DOE approach and the procedure subsequently applied to the use of a bipyridyl-tagged amine as an isocyanate scavenger. Preliminary results clearly demonstrate the potential of using chelation tags such as bipyridine units as a means for removing solution phase reagents and scavengers from reaction mixtures providing an attractive alternative to their resin-bound and fluorous-tagged counter-parts. 相似文献