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1.
用荧光光谱法研究了锌离子(Zn2+)与牛血清白蛋白(BSA)的作用机制.实验表明,Zn2+对BSA具有荧光猝灭作用,其猝灭方式为静态猝灭.求出了猝灭常数、结合常数及结合位点数.利用同步荧光光谱研究了Zn2+对BSA分子荧光光谱的影响. 相似文献
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烟碱与牛血清白蛋白相互作用的光谱研究 总被引:18,自引:2,他引:16
在0.1 mol·L-1的磷酸氢二钠-柠檬酸体系中,采用荧光光谱、紫外吸收光谱研究了牛血清白蛋白(BSA)与烟碱的相互作用。荧光滴定表明这种相互作用使BSA的内源荧光猝灭,尼古丁和BSA形成1∶1稳定复合物。不同温度和酸度下的猝灭作用证实其静态猝灭行为和疏水作用机制。紫外吸收光谱和同步荧光光谱表明,相互作用引起BSA构象变化,而同步荧光光谱提示结合位点更接近于色氨酸。 相似文献
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金雀花碱(Cy)是一种生物碱,主要存在于豆科毒豆属植物种子中。Cy具有较强的生物活性,特别是作为戒烟药物已得到广泛应用。在模拟生理条件下,应用荧光光谱法研究了Cy同牛血清白蛋白(BSA)之间的相互作用以及Cy猝灭BSA荧光发射的机理。详细考查了水浴温度、水浴时间以及溶液pH等因素对荧光猝灭的影响,并且通过Stem-Volmer方程计算了Cy与BSA间的结合类型、结合位点数目以及结合常数。结果表明,Cy与BSA可形成摩尔比为1∶1的非共价复合物,其结合常数为5.6×103,其猝灭类型为静态猝灭。同步荧光光谱研究结果表明,Cy的结合主要影响BSA 的Trp残基的荧光发射。进一步应用分子对接研究表明,氢键与疏水作用是Cy与BSA形成复合物的主要推动力。Cy与BSA中Trp213及其周围的氨基酸残基间存在氢键与疏水作用,这种作用将改变Trp213所处微环境的疏水情况,从而导致BSA的荧光发生猝灭。 相似文献
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防己诺林碱与牛血清白蛋白相互作用的研究 总被引:8,自引:3,他引:5
在不同温度下,用荧光猝灭光谱、同步荧光光谱和紫外-可见吸收光谱,研究了防己诺林碱与BSA相互作用的光谱学行为。防己诺林碱对BSA有较强的荧光猝灭作用。根据不同温度下防己诺林碱对BSA的荧光猝灭作用,利用Stern-Volmer方程处理实验数据,表明防己诺林碱对BSA的荧光猝灭作用属于静态猝灭。根据Frster非辐射能量转移理论计算出了防己诺林碱与BSA间的结合距离R(27 ℃ 2.51 nm; 37 ℃ 2.72 nm; 47℃ 2.89 nm)、结合常数KA(27 ℃ 1.05×105 L·mol-1; 37 ℃ 3.31×105 L·mol-1; 47 ℃ 7.24×105 L·mol-1)及对应温度下的热力学参数。热力学数据表明二者主要靠疏水作用力结合,同时用同步荧光光谱探讨了防己诺林碱对BSA构象的影响。 相似文献
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在不同温度下,采用荧光光谱,紫外-可见吸收光谱研究了虎杖苷与牛血清白蛋白(BSA)的相互作用。虎杖苷对BSA的荧光有较强的猝灭作用。根据292K和311K时虎杖苷对BSA的荧光猝灭作用,利用Stern-Volmer方程及双倒数方程处理实验数据,表明虎杖苷对BSA的荧光猝灭作用属于动态猝灭过程,根据Foerster非辐射能量转移理论计算出了虎杖苷与BSA间的结合距离r=3.61nm,结合常数(Kb)分别为5.189×105L·mol-1(292K),1.382×105L·mol-1(311K)及对应温度下的热力学参数。实验结果表明二者主要靠疏水作用力结合,采用同步荧光光谱探讨了虎杖苷对BSA构象的影响。 相似文献
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采用荧光光谱、同步荧光光谱、紫外光谱研究了水溶液中头孢噻肟钠(CTX)与牛血清白蛋白(BSA)之间的相互作用.结果表明:CTX能显著猝灭BSA的内源荧光并以静态猝灭为主;两者以摩尔比1∶1结合,结合常数分别为1.20×104(298K)、7.63×103(308K)和6.69×103(313K)L·mol-1;根据热力学参数判断CTX与BSA之间的作用力主要为氢键和范德华力;依据Foester非辐射能量转移理论计算出CTX与BSA之间的结合距离r为3.26nm,同步荧光光谱研究表明CTX能够使BSA构象发生变化. 相似文献
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运用荧光光谱法研究了几种EGCG金属配合物(EGCG-M)与牛血清白蛋白(BSA)的相互作用。实验结果表明,几种EGCG-M对BSA的荧光光谱有明显猝灭作用,两者之间的相互作用为静态猝灭,并得到了静态猝灭常数、结合常数及结合位点数等数据。 相似文献
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Europium salts were dissolved in poly(ethylene oxide) (PEO) oligomers. The europium ions were revealed to be electrochemically redox-active in PEO oligomers. Trivalent europium ions (Eu3+) showed red fluorescence (λem = 614 nm) in PEO oligomers, and a new intensive blue fluorescence (λem = 450 nm) was obtained after reducing the Eu3+ into Eu2+ by giving negative potential (−0.7 V versus Ag). Reversible fluorescence switching was also carried out by changing the given potential alternately. Sufficient color change required about 20 min because of slow electrode reaction. The fluorescence intensity from europium ions was affected by the molecular weight of matrix PEO. The amount of terminal hydroxyl groups and local viscosity were revealed to affect the fluorescence as the vibrational quenching and diffusion coefficient, respectively. The PEO oligomer with average molecular weight of 600 gave the most intensive fluorescence. 相似文献
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In order to obtain dense and stable poly(ethylene oxide) (PEO) layers for reducing protein adsorption, polystyrene (PS) plates were first soaked in chloroform/methanol mixed organic solvent to swell the polymer. The swelled PS plates were then immersed into Pluronic F127 (amphiphilic block copolymer) aqueous solution, Pluronic F127 molecules were adsorbed favorably on the swelled PS surfaces. After evaporation of organic solvent, the adsorbed Pluronic F127 molecules were trapped and anchored permanently on the PS substrates. The dense and stable PEO layers anchored on the PS surfaces can effectively inhibit protein adsorption. 相似文献
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A. Karanikolas P. Tsolakis G. Bokias C. Tsitsilianis 《The European physical journal. E, Soft matter》2008,27(3):335-343
The self-organization of the double hydrophilic triblock copolymer poly(ethylene oxide)-b-poly(2-vinylpyridine)-b-poly(ethylene
oxide), PEO-b-P2VP-b-PEO, was investigated in dilute aqueous solution under several experimental conditions using turbidimetry,
as well as static and dynamic light scattering. As a result of the temperature-sensitive properties of the end PEO blocks
and the p H-responsive properties of the middle P2VP block, the formation of large star-like micellar nanostructures is observed at
high p H, while at low p H, but in the presence of salt and at high temperature, flower-like micelles are formed. Moreover, the viscosimetric and
dynamic light scattering studies at low p H revealed that micelle-like nanostructures are formed upon mixing the triblock copolymer with poly(acrylic acid), PAA, due
to hydrogen bonding interpolymer complexation. 相似文献
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Haishan Bu Songhua Shi Erqiang Chen Hanjie Hu Ze Zhang Bernhard Wunderlich 《Journal of Macromolecular Science: Physics》2013,52(5):731-747
Single-molecule single crystals were prepared from two fractions of poly(ethylene oxide) (PEO) with narrow molar mass distribution and an equimolar mixture of the two fractions. It was proven that the molar mass distribution of the single-molecule single crystals from the mixed sample corresponds to an addition of those of the pure fractions. Well-shaped crystals were obtained after isothermal crystallization or on annealing. A variety of morphologies typical for multimolecule single crystals of PEO were found and are described on the basis of the various known modes of twinning. The results are in agreement with the known unit cell of PEO. 相似文献
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The ionic conductivity of poly(ethylene oxide) and poly(propylene oxide) in pure solution form, individually complexed with
salts of Na+ and Li+, with and without plasticizer (propylene carbonate) and in blended form with individual salt with and without plasticizer,
was studied. The conductance measurements were made at various concentrations of salt polymer complexes and at different temperatures.
The effects of temperature and plasticizer concentration were measured from Arrhenius conductance plots. It is shown that
the addition of salts in pure PEO increases conductance many times. The plasticizer has also same effect. The blending of
PEO with PPO gives enhanced conductivity as compared to pure PEO. The activation energies were determined for all the systems
which gave higher values for pure PEO and the value decreases with the addition of Li and Na salts and further decreases with
the addition of plasticizer. The blending has also lowered the activation energy values which mean that incorporation of PPO
in PEO has decreased crystallinity and the amorphous region has increased the local mobility of polymer chains resulting in
lower activation energies. 相似文献
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The formation of an intramolecular excimer of 1,10-bis(1-pyrene)decane in sodium dodecyl sulfate (SDS)/bovine serum albumin (BSA) and SDS/poly(ethylene oxide) (PEO) solutions was studied by steady-state and time-resolved fluorescence methods to determine the effect of viscosity. The viscosity-dependent ratio between intramolecular excimer and monomer fluorescence intensities of 1,10-bis(1-pyrene)decane was obtained by steady-state fluorescence measurements. The results show that in micelle-like clusters formed in SDS/BSA and SDS/PEO systems, the microviscosity is higher than in free micelles. It was found that Birks' kinetic model was not valid in this case, and the monomer and excimer fluorescence decays had to be fitted by a sum of three exponentials. The excimer formation kinetics in constrained systems is more complex than in homogeneous solutions, but it is possible to find an empirical parameter depending on viscosity. 相似文献
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Electrochemical characteristics of the dimercaptan-poly(ethylene oxide) grafted polyaniline electrodes 总被引:1,自引:0,他引:1
The electrochemical and transport properties of the 2,5-dimercapto-1,3,4-thiadiazole (DMcT)/poly(ethylene oxide) (PEO) grafted polyaniline electrodes and the DMcT/polyaniline electrode interfaced with the poly(acrylonitrile) (PAN) based solid-polymer-electrolyte (SPE) containing lithium perchlorate and ethylene carbonate were studied. Compared with the electrochemical and transport properties of the DMcT/polyaniline electrode, the capacitance and voltammetric current density, obtained by cyclic voltammetry, were increased for the electrode with low grafted polyaniline (less than 3 mol. %), while decreased when the applied copolymers were highly grafted ones. The charge transfer resistance obtained from impedance measurements was much smaller in the DMcT/PEO grafted polyaniline electrode than that in the DMcT/polyaniline electrode, and more pronounced reduction of charge transfer resistance was observed for the electrode with low grafted polyaniline. The diffusion coefficient of lithium cation in the electrode was increased when the PEO grafted polyaniline was used as an electrode material, however, the increase of the diffusion coefficient was less significant at higher graft degrees. All these changes in electrochemical and transport characteristics by the employment of PEO chains upon polyaniline backbones were attributed to the enhancement of lithium ion solvation and enlarged free volume in the electrode. 相似文献
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M. Dosiere 《Journal of Macromolecular Science: Physics》2013,52(3-4):303-328
The phase diagrams of the binary systems poly(ethylene oxide) (PEO)-resorcinol and poly(ethylene oxide)-p-nitrophenol show the presence of molecular complexes with well-defined stoichiometries. The crystal structure of these two molecular complexes has been determined from wide-angle x-ray diffraction patterns of stretched films and spherulites. The morphology of the two complexes crystallized from the melt is investigated by differential scanning calorimetry and small-angle x-ray scattering. The crystallization of the PEO-resorcinol complex from the melt gives integral-folded crystals with either extended chains (EC) or n-folded chains (n-FC). As observed for PEO oligomers, the fraction of EC crystals of the PEO-resorcinol complex increases with the crystallization temperature to give finally only EC crystals but in a larger range of crystallization temperatures than for pure PEO. On the other hand, the PEO-p-nitrophenol complex crystallizes over all the studied crystallization range as stable nonintegral-folded (NIF) crystals. Two proposals related to the crystal structure of these complexes and their mode of growth are invoked to explain these two greatly different morphologies at the lamellar level. 相似文献
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Z.M. Elimat 《辐射效应与固体损伤》2013,168(8):686-695
The optical characterization of poly (ethylene oxide)/zinc oxide thin films has been done by analyzing the absorption spectra in the spectral wavelength region 380–800 nm using a ultraviolet-spectrophotometer at room temperature. Thin film polymer composites made of poly (ethylene oxide) (PEO) containing zinc oxide (ZnO) filler concentrations (0%, 2%, 6%, 10%, and 14%) by weight were used in this study. The optical results obtained were analyzed in terms of the absorption formula for non-crystalline materials. The optical energy gap and other basic optical constants such as dielectric constants and optical conductivity were investigated and showed a clear dependence on the ZnO filler concentration. It was found that the optical energy gap for the composite films is less than that for the neat PEO, and that it decreases as the ZnO concentration increases. Enhancement of the optical conductivity was observed with increase in the ZnO concentration. Dispersion of refractive index was analyzed using the Wemple–DiDomenico single oscillator model. The refractive index (n), extinction coefficient (k), and dispersion parameters (Eo, Ed) were calculated for the investigated films. 相似文献