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| 胡椒碱与牛血清白蛋白的相互作用 | |
| 引用本文： | 尚永辉,李华,孙家娟.胡椒碱与牛血清白蛋白的相互作用[J].光谱实验室,2011,28(3):1236-1238. |
| 作者姓名： | 尚永辉 李华 孙家娟 |
| 作者单位： | 1. 咸阳师范学院化学与化工学院,陕西省咸阳市文林路东段1号,712000;西北大学分析科学研究所,西安市,710069 2. 西北大学分析科学研究所,西安市,710069 3. 咸阳师范学院化学与化工学院,陕西省咸阳市文林路东段1号,712000 |
| 基金项目： | 国家自然科学基金，西北大学研究生交叉学科资助项目，陕西省教育厅科研计划项目，咸阳师范学院专项科研基金 |
| 摘 要： | 采用荧光光谱技术研究了胡椒碱与牛血清白蛋白（BSA）的相互作用。根据测定不同温度下胡椒碱对BSA的猝灭常数,证实了荧光猝灭过程为静态猝灭,由热力学参数焓变（ΔH）小于零和熵变（ΔS）大于零,推断出胡椒碱与BSA之间主要靠静电引力相结合,生成自由能变（ΔG）为负值,表明胡椒碱与BSA的作用过程是一个自发过程;并应用同步荧光光谱技术考察了胡椒碱对BSA构象的影响。 |
| 关 键 词： | 胡椒碱 牛血清白蛋白 荧光光谱 |
The Interaction Between Piperine and Bovine Serum Albumin |
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| SHANG Yong-Huia,b LI Huab SUN Jia-Juan.The Interaction Between Piperine and Bovine Serum Albumin[J].Chinese Journal of Spectroscopy Laboratory,2011,28(3):1236-1238. | |
| Authors: | SHANG Yong-Huia b LI Huab SUN Jia-Juan |
| Institution: | aa(School of Chemistry ＆ Chemical Engineering,Xianyang Normal University,Xianyang,Shaanxi 712000,P.R.China)b(Institute of Analytical Science,Northwest University,Xi＇an 710069,P.R.China) |
| Abstract: | The interaction between piperine and bovine serum albumin（BSA） was studied by fluorescence spectroscopy.The quenching constants were obtained at different temperatures,the results indicated that the fluorescence quenching mechanism for BSA through piperine binding was likely a static quenching process.The thermodynamic parameters,enthalpy change ΔH0 and entropy change ΔS0,which indicated that the interaction of piperine with BSA was driven mainly by electrostatic force.The process of binding was a spontaneous process in which Gibbs free energy change ΔG0.The effect of piperine on the conformation of BSA was analyzed using synchronous fluorescence spectroscopy. |
| Keywords: | Piperine Bovine Serum Albumin Fluorescence Spectroscopy |
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