毛细管电泳法分离测定芦丁、槲皮素和连翘苷

用毛细管电泳紫外检测法同时测定了芦丁、槲皮素和连翘苷，研究了各种条件的影响，得到了优化的实验条件，在20mmol/L的Na2B4O7(H3B03调节至pH8．40)-30mmol/L十二烷基硫酸钠-10％乙腈(1 9)的缓冲溶液中，分离电压为12kV时，芦丁、槲皮素和连翘苷在10min内得到了良好的分离，检测波长为254nm，芦丁、槲皮素和连翘苷分别在0．01-1．0mg/mL，0．01-1．0mg／mL和0．05-1．0mg/mL质量浓度范围内与电泳峰高呈现良好线性关系，检测下限分别为0．005mg/mL，0．005mg/mL和0．01mg／mL，应用于实际样品的测定。     

毛细管电泳法测定复方芦丁片的芦丁和维生素C

用毛细管电泳紫外检测法同时测定了复方芦丁片中芦丁和抗坏血酸的含量,研究了各种条件的影响,得到了优化的实验条件,在30mmol/L Na2B2O7-H3BO3(pH7.50)缓冲溶液中,芦丁和维生素C在13min内得到了良好的分离,芦丁和维生素C分别在0.5-0.005mg和5.0-0.05mg浓度范围内与电泳峰高呈现良好线性关系,检测下限分别为0.002mg和0.01mg,应用于实际样品的测定。     

高效液相色谱法测定复方芦丁片中芦丁和维生素C的含量

采用反相高效液相离子对色谱法,以对氨基苯甲酸为内标,测定了复方芦丁片中芦丁和维生素Ｃ的含量。色谱柱为μ－ＢｏｎｄａｐａｋＣ_（１８）,流动相为甲醇－水（５０：５０）配制的樟脑磺酸溶液（０．００２５ｍｏｌ／Ｌ）,检测波长为２５４ｎｍ。并做了线性范围和回收率测定。     

毛细管电泳法测定复方芦丁片中的芦丁和维生素C

用毛细管电泳紫外检测法同时测定了复方芦丁片中芦丁和抗坏血酸的含量 ,研究了各种条件的影响 ,得到了优化的实验条件 ,在 30 mmol/L Na2 B4 O7-H3BO3( p H7.5 0 )缓冲溶液中 ,芦丁和维生素 C在 1 3min内得到了良好的分离 ,芦丁和维生素 C分别在 0 .5～ 0 .0 0 5 mg和 5 .0～ 0 .0 5 mg浓度范围内与电泳峰高呈现良好线性关系 ,检测下限分别为 0 .0 0 2 mg和 0 .0 1 mg,应用于实际样品的测定     

复方芦丁片中主要成分的毛细管电泳安培检测

用毛细管电泳安培检测法同时测定了复方芦丁片中芦丁和抗坏血酸的含量 ,研究了各种实验条件对分离效果的影响 ,得到了优化的实验条件。以直径为 5 0 μm的碳纤维微电极为工作电极 ,电极电位为+ 0 .80V(vs.Ag AgCl) ,4 0mmol L的Na2 B4O7 H3 BO3 (pH值为 7 5 0 )为缓冲溶液。在此条件下 ,芦丁和维生素C在 1 0min内得到了良好的分离。芦丁和维生素C分别在 5 .0×1 0 - 8～ 5 .0× 1 0 - 4 g mL、8.0× 1 0 - 8～ 5 .0× 1 0 - 4g mL浓度范围内与电泳峰电流呈现良好的线性关系 ;检测下限分别为 2 .0× 1 0 - 8、5 .0× 1 0 - 8g mL。方法应用于实际样品的测定 ,结果令人满意     

小波变换分光光度法同时测定复方芦丁片中芦丁和维生素C

建立了同时测定复方芦丁片中芦丁和维生素C含量的计算分光光度法--小波变换分光光度法.对芦丁和维生素C的重叠吸收光谱进行连续小波变换,分别提取只与芦丁和维生素C有关的特征小波变换系数,由特征小波变换系数值和浓度建立的线性回归方程,可以准确地测定复方芦丁片中芦丁和维生素C的含量.芦丁和维生素C的线性范围分别为0～25 mg·L-1和0～15 mg·L-1,对3个模拟试样作回收率和精密度试验,回收率在98.1%～102.0%之间,RSD值均小于等于0.9%.     

毛细管电泳法测定银杏叶片中的橙皮素、芦丁和槲皮素

建立了一种利用毛细管电泳法测定银杏叶片中橙皮素、芦丁和槲皮素的方法.研究了缓冲溶液pH和浓度、分离电压和进样时间对分离的影响,在优化的条件下,10 min内实现了三种物质的良好分离.橙皮素、芦丁和槲皮素分别在0.03～0.80、0.06～1.00,0.04～0.90 mg/mL浓度范围内和峰电流成线性关系,线性相关系数分别为0.9992、0.9976和0.999l,检出限分别为0.005、0.009和0.006 mg/mL.     

复方维生素B片中主要成分的高效毛细管电泳电化学法检测

采用高效毛细管电泳－电化学检测法同时测定复方维生素B片中的主要成分维生素B1，B12，B6和C的含量；研究了电极电位，运行缓冲溶液的浓度和酸度，电泳电压和进样时间等对电泳的影响，以微铂电极为工作电极，检测电位＋0．5V（vs SCE)，在pH9.0的15mmol/L Tris-1mmol/L H3BO3缓冲溶液中，上述4组分在5min内获得基线分离；维生素B1，B12，B6和C的线性范围分别为2．1mg/L-1.0g/L,6.0mg/L-0.80g/L,1.4mg/L-0.72g/L和0．97mg/L-0.44g/L检出限分别为0．50mg/L,1.0mg/L,,0.65mg/L和0．40mg/L；5次测定峰高的相对标准偏差分别为2．4％，3．0％，3．1％，和2．5％，5次测定的平均回收率分别为99％，102％，98％和100％。     

高效毛细管电泳电导法快速检测复方维生素B片中的VBl、VBl2、VB6和VC

采用高效毛细管电泳电导法同时分离、测定了复方维生素B片中的主要成分VB1，VB12，VB6和VC的含量。研究了运行缓冲溶液的酸度和浓度、电泳电压、进样时间等因素对电泳的影响。在优化的实验条件下：40mmol/L Tris-4mmol/L H3BO3(pH8．0)的缓冲溶液中加入0．30mmol/L CTAB(溴化十六烷基三甲基铵)，分离电压为15kV，上述4组分在5min内得到良好的分离。维生素B1，B12，B6和VC的线性范围分别为5．5～1．0mg/mL；15～1．5mg／mL;1．0～0．40mg／mL和6．6～0．80mg／mL；检测限分别为0．80μg/mL，4．0μg/mL，0．50μg/mL，2．9μg/mL；5次测定峰高的相对标准偏差分别为2．2％，1．6％，3．9％，2．8％。5次测定的平均回收率分别为99％，94％，l00％，97％。     

高效毛细管电泳法同时测定药品中苯甲酸和山梨酸钾

建立了毛细管电泳-紫外检测法测定硝酸咪康唑乳膏、小儿止咳糖浆及复方苦参水杨酸散中苯甲酸和山梨酸钾的方法。在230nm波长处以焦性没食子酸为内标物,分离电压为20kV,分离温度为25℃,用20mmol/L硼砂缓冲液(pH9.2)作毛细管电泳的运行液,被测组分与内标物得到快速分离。苯甲酸和山梨酸钾的进样质量浓度在1～400mg/L范围内与电泳峰面积呈良好的线性关系,相关系数r均为0.9999,检出限均为0.15mg/L。方法可用于药品中苯甲酸和山梨酸钾含量的测定。     

Oscillopolarography of rutin

Summary It has been shown that in the oscillopolarography of rutin the electrochemical reduction is affected by the pH of the solution and the concentration of ethanol. Protonation of the rutin molecules and their adsorption on the electrode in aqueous solutions are observed. The adsorption is reduced by increasing the concentration of ethanol to 50%.The change in current at various pH values with a constant concentration of rutin is due to a change in the concentration of protonated and ionized polarographically active forms of rutin.Conditions for the quantitative analysis of rutin have been found.Pyatigorsk Pharmaceutical Institute. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 187–192, March–April, 1978.     

Das rutin als anorganisch-analytisches reagens: Nachweis von vanadium(v) mit rutin

A simple, sensitive and highly specific test is presented for the detection of vanadium(V). The test is based on the colour reaction of vanadium(V) with rutin (quercetin-3-rutinosude) in the presence of the ammoniumperoxydisulfate in phosphoric acid.Sensitivity : 0.3 μg vanadium (V) in 1 ml solution.     

Enzymatic method of obtaining rutin

The possibility has been shown for the first time of using enzymes for increasing the yield of rutin. The processes of fermentation and extraction have been optimized with the aid of mathematical methods of planning experimentation.All-Union Scientific-Research Chemical and Technological Institute of the Medicinal and Microbiological Industry, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 628–630, September–October, 1991.     

Theoretical conformational analysis of rutin

Abstract  

The structural properties of rutin were determined by using a computational multistep progression. In the first step a stochastic strategy based on a molecular mechanics calculation was adopted to obtain a preliminary screening of the low-energy rutin conformations. The most stable structures obtained by the random conformational search were used as a starting point for an Austin Model 1 (AM1) semiempirical optimization. After this treatment, 16 structures characterized by a more stable frontal in respect to back disposition of the glycosidic chain of rutin were identified. To validate the results found from the stochastic search a molecular dynamics simulation was carried out. The results evidenced the presence of a global minimum highly stabilized by a hydrogen bond between the hydroxyl in the 3′ position of the B ring and the endocyclic oxygen of the rhamnose unit followed by approximately 8 kJ mol⁻¹ less stable local minima with similar energy values. Finally, the reliability of the molecular model was confirmed by comparing the calculated electronic absorption spectrum with that measured on a methanolic rutin solution.     

Enzymatic method of obtaining rutin

The possibility has been shown for the first time of using enzymes for increasing the yield of rutin. The processes of fermentation and extraction have been optimized with the aid of mathematical methods of planning experimentation.