共查询到19条相似文献,搜索用时 46 毫秒
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合成了具有抗菌活性的β-氨基酸聚合物,采用核磁共振和凝胶渗透色谱表征聚合物的数均分子量,通过最低抑菌浓度实验测试了β-氨基酸聚合物对多种革兰氏阳性菌(包括多株耐药菌)的抗菌活性,并开展了可能的耐药性研究。通过细胞膜去极化实验和扫描电子显微镜探究了抗菌聚合物对革兰氏阳性菌,尤其是对耐甲氧西林金黄色葡萄球菌的抗菌机理。结果表明,β-氨基酸聚合物通过作用于细菌的细胞膜杀死革兰氏阳性菌从而获得高效的抗菌活性,其对枯草芽孢杆菌的抗菌活性最好,最低抑菌浓度为3.13μg/mL。金黄色葡萄球菌对β-氨基酸聚合物不产生耐药性,但在相同条件下测试的对照抗生素诺氟沙星的最低抑菌浓度增加了124倍,表明细菌对抗生素很快产生了耐药性。 相似文献
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聚(4-乙烯基吡啶季铵盐-丙烯酰胺)的抗菌性能与机理研究 总被引:2,自引:0,他引:2
季铵盐型阳离子聚合物具有絮凝、缓蚀与杀菌等多种功能,据此,我们通过分子设计,先将丙烯酰胺与4-乙烯基吡啶(4-VP)进行共聚合,然后使用季铵化试剂硫酸二甲酯使共聚物阳离子化,制备了吡啶季铵盐型阳离子聚丙烯酰胺(QPAV),本文报道QPAV的抗菌性能,并探讨其抗菌机理,结果表明,吡啶季铵盐型阳离子聚丙烯酰胺具有很强的抗菌性能,且其抗菌机理是基于杀菌而不仅仅是抑菌。 相似文献
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合成了一种甲基丙烯酰胺类两性离子单体,采用自由基聚合法制备了具有双重抗菌作用的水凝胶。利用1 H-NMR和FT-IR表征单体和水凝胶,考察了水凝胶释放水杨酸的动力学曲线。以导入绿色荧光蛋白的大肠杆菌为试验菌,荧光显微镜观察水凝胶表面细菌黏附情况;以金黄色葡萄球菌和大肠杆菌为试验菌,研究水凝胶抗菌性能。结果表明,该水凝胶可有效防止细菌黏附并且通过释放水杨酸实现抗菌功能。 相似文献
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由于传统抗生素类药物容易使细菌产生耐药性而成为超级细菌,新型的抗菌药物亟待开发。通过模拟天然抗菌多肽设计合成的高分子抗菌剂,具有很高的抗菌活性和生物选择性,而且由于其合成方法简单,结构易于控制,且可实现大规模工业生产,有望成为代替传统抗生素和抗菌多肽的新一代抗菌药物。本文介绍了天然抗菌多肽的抗菌机理与模拟天然抗菌多肽的... 相似文献
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合成了一种甲基丙烯酰胺类两性离子单体,采用自由基聚合法制备具有双重抗菌作用的水凝胶。利用1 H-NMR和FT-IR表征单体和水凝胶,考察了水凝胶释放水杨酸的水解曲线。以导入绿色荧光蛋白的大肠杆菌为试验菌,荧光显微镜下观察水凝胶表面的细菌黏附情况;以金黄色葡萄球菌和大肠杆菌为试验菌,研究水凝胶的抗菌性能。结果表明,该水凝胶可有效防止细菌黏附并且通过释放水杨酸实现抗菌性能。 相似文献
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Shady Farah Wahid Khan Ira Farber Dana Kesler‐Shvero Nurit Beyth Ervin I. Weiss Abraham J. Domb 《先进技术聚合物》2013,24(5):446-452
Quaternary ammonium polyethylenimine (QA‐PEI) nanoparticles were synthesized by PEI crosslinking and alkylation with octyliodide followed by quaternization by methyl iodide. The present study focuses on the reproducibility of particles formation with respect to particle size, positive charge, oxidative, thermal stability and antibacterial activity. The QA‐PEI‐based nanoparticles with particle size in the range of 160–190 nm were synthesized in high reproducibility and showed high chemical stability against environmental changes including exposure to different oxidizers and storage conditions. QA‐PEI nanoparticles incorporated in dental restorative resin composites at 2% w/w demonstrated strong antibacterial activity. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
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Bio‐Inspired Coating Strategies for the Immobilization of Polymyxins to Generate Contact‐Killing Surfaces 下载免费PDF全文
Microbial colonization of indwelling devices remains a major concern in modern healthcare. Developing approaches to prevent biomaterial‐associated infections (BAI) is, therefore, in great demand. This study aimed to immobilize two antimicrobial peptides (polymyxins B and E) onto polydimethylsiloxane (PDMS) using two polydopamine (pDA)‐based approaches: the conventional two‐step method involving the deposition of a pDA layer to which biomolecules are immobilized, and a one‐step method where peptides are dissolved together with dopamine before its polymerization. Surface characterization confirms the immobilization of polymyxins onto PDMS at a non‐toxic concentration. Immobilization of polymyxins using a one‐step pDA‐based approach is able to prevent Pseudomonas aeruginosa adhesion and kill a significant fraction of the adherent ones. Living cells adhered to these modified surfaces exhibit the same susceptibility pattern as cells adhered to unmodified surfaces, highlighting no resistance development. Results suggest that polymyxins immobilization holds a great potential as an additional antimicrobial functionality in the design of biomaterials.
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Felix Unglaube Alexander Lammers Dr. Carsten Robert Kreyenschulte Prof. Dr. Michael Lalk Dr. Esteban Mejía 《ChemistryOpen》2021,10(12):1244-1250
Rice husk, one of the main side products in the rice production, and its sustainable management represent a challenge in many countries. Herein, we describe the use of this abundant agricultural bio-waste as feedstock for the preparation of silver-containing carbon/silica nano composites with antimicrobial properties. The synthesis was performed using a fast and cheap methodology consisting of wet impregnation followed by pyrolysis, yielding C/SiO2 composite materials doped with varying amounts of silver from 28 to 0.001 wt %. The materials were fully characterized and their antimicrobial activity against ESKAPE pathogens, namely E. faecium, S. aureus, K. pneumoniae, A. baumannii, P. aeruginosa, and E. coli, and the pathogenic yeast C. albicans was investigated. Sensitivities of these strains against the prepared materials were demonstrated, even with exceptional low amounts of 0.015 m% silver. Hence, we report a straightforward method for the synthesis of antimicrobial agents from abundant sources which addresses urgent questions like bio-waste valorization and affordable alternatives to increasingly fewer effective antibiotics. 相似文献
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Gayan Heruka De Zoysa Kelvin Wang Jun Lu Yacine Hemar Vijayalekshmi Sarojini 《Molecules (Basel, Switzerland)》2020,25(24)
Novel antibiotic treatments are in increasing demand to tackle life-threatening infections from bacterial pathogens. In this study, we report the use of a potent battacin lipopeptide as an antimicrobial gel to inhibit planktonic and mature biofilms of S. aureus and P. aeruginosa. The antimicrobial gels were made by covalently linking the N-terminal cysteine containing lipopeptide (GZ3.163) onto the polyethylene glycol polymer matrix and initiating gelation using thiol-ene click chemistry. The gels were prepared both in methanol and in water and were characterised using rheology, Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). Antibacterial and antibiofilm analyses revealed that the gels prepared in methanol have better antibacterial and antibiofilm activity. Additionally, a minimum peptide content of 0.5 wt% (relative to polymer content) is required to successfully inhibit the planktonic bacterial growth and disperse mature biofilms of P. aeruginosa and S. aureus. The antibacterial activity of these lipopeptide gels is mediated by a contact kill mechanism of action. The gels are non-haemolytic against mouse red blood cells and are non-cytotoxic against human dermal fibroblasts. Findings from this study show that battacin lipopeptide gels have the potential to be developed as novel topical antibacterial agents to combat skin infections, particularly caused by S. aureus. 相似文献
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The worsening situation of global drug resistance is urgently demanding for novel antimicrobial agents. Considerable efforts have been concentrated on developing new antibacterial therapies with new mode of actions, exerting no selective pressure on bacterial mutation, and minimizing toxicity to host cells. In this context, active targeting can greatly contribute to selectivity between pathogens and mammalian cells in which carbohydrates, playing important roles in numerous biological processes, can be employed as targeting ligands or “trojan horse.” This short account has discussed the recent results of carbohydrate-based antimicrobial agents developed by our group. Other excellent works by other scientists and possible directions in the future are also discussed. 相似文献
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Antimicrobial peptides (AMPs) are being intensively investigated as they are considered promising alternatives to antibiotics where their clinical efficacy is dwindling due to the emergence of antimicrobial resistance (AMR). Accompanying with the development of AMPs, a number of fluorescent probes have been developed to facilitate the understanding the modes of action of AMPs. These probes have been used to monitor the binding process, determine the working mechanism and evaluate the antimicrobial properties of AMPs. In particular, with the recent advance of aggregation-induced emission (AIE) fluorophores, that show many advantageous properties over traditional probes, there is an increasing research interest in using AIE probes for AMP studies. In this review, we give an overview of AMP development, highlight the recent progress of using fluorescence probes in particularly AIE probes in the AMP field and propose the future perspective of developing potent antimicrobial agents to combat AMR. 相似文献
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Photodynamic inactivation of microorganisms has gained substantial attention due to its unique mode of action, in which pathogens are unable to generate resistance, and due to the fact that it can be applied in a minimally invasive manner. In photodynamic therapy (PDT), a non-toxic photosensitizer (PS) is activated by a specific wavelength of light and generates highly cytotoxic reactive oxygen species (ROS) such as superoxide (O2−, type-I mechanism) or singlet oxygen (1O2*, type-II mechanism). Although it offers many advantages over conventional treatment methods, ROS-mediated microbial killing is often faced with the issues of accessibility, poor selectivity and off-target damage. Thus, several strategies have been employed to develop target-specific antimicrobial PDT (aPDT). This includes conjugation of known PS building-blocks to either non-specific cationic moieties or target-specific antibiotics and antimicrobial peptides, or combining them with targeting nanomaterials. In this review, we summarise these general strategies and related challenges, and highlight recent developments in targeted aPDT. 相似文献
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Neeraj Kumar Gajendra Smgh Ashok K Yadav 《Phosphorus, sulfur, and silicon and the related elements》2013,188(1):217-225
Abstract 2-Thioxc-3,5,7-trisubstitutcd-I-[2′.3′,5′-tri-O-benzoyl-β-D-ribofuranosyllpyrido [2,3-d]pyri midin-4(IH)-ones have been prepared by the condensation of trimethylsilyl derivative of 2-thioxo-3,5,7-trisubstituted pyrido[2,3-d]pyrimidin-4(IH)-ones with β-D-ribofuranose 1-acetate-2,3,5-tribenzoate in 65%-78% yield. The structure of the synthesized ribofuranosides and their precursors have been established by IR, 1H NMR and elemental analysis. These derivatives have been screened for their antimicrobial activity. 相似文献
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Luminita Georgeta Confederat Cristina Gabriela Tuchilus Maria Dragan Mousa Shaat Oana Maria Dragostin 《Molecules (Basel, Switzerland)》2021,26(12)
Despite the advantages presented by synthetic polymers such as strength and durability, the lack of biodegradability associated with the persistence in the environment for a long time turned the attention of researchers to natural polymers. Being biodegradable, biopolymers proved to be extremely beneficial to the environment. At present, they represent an important class of materials with applications in all economic sectors, but also in medicine. They find applications as absorbers, cosmetics, controlled drug delivery, tissue engineering, etc. Chitosan is one of the natural polymers which raised a strong interest for researchers due to some exceptional properties such as biodegradability, biocompatibility, nontoxicity, non-antigenicity, low-cost and numerous pharmacological properties as antimicrobial, antitumor, antioxidant, antidiabetic, immunoenhancing. In addition to this, the free amino and hydroxyl groups make it susceptible to a series of structural modulations, obtaining some derivatives with different biomedical applications. This review approaches the physico-chemical and pharmacological properties of chitosan and its derivatives, focusing on the antimicrobial potential including mechanism of action, factors that influence the antimicrobial activity and the activity against resistant strains, topics of great interest in the context of the concern raised by the available therapeutic options for infections, especially with resistant strains. 相似文献