共查询到19条相似文献,搜索用时 109 毫秒
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以宽谱带高压汞灯为光源的倒置荧光显微镜,配备电荷耦合器件(CCD)及微流控芯片,自组装芯片毛细管电泳荧光电荷耦合器件检测系统。选择不同激发波长(340~540 nm)荧光试剂,进行荧光检测,荧光素的检出限(S/N=3)为7.3×10-9mol/L,荧光素异硫氰酸酯(FITC)的检出限(S/N=3)为1.7×10-8mol/L,显示了该系统对荧光试剂选择范围宽、灵敏度高的特点。用该系统成功分离了FITC与荧光素、曙红与荧光素两种混合荧光试剂。实验表明,组装的芯片毛细管电泳CCD荧光检测系统是成功的。 相似文献
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一种以发光二极管为激发光源的荧光检测器 总被引:5,自引:0,他引:5
利用高亮度发光二极管作为激发光源组装了一荧光检测器并应用于毛细管电泳检测 ;激发光斑与毛细管检测池的耦合通过透镜加光阑组合方式实现 ;光纤用于收集并传输荧光信号 ,增加了光学系统的灵活性和紧凑性 ;光阑、检测池和光纤之间的校准简单、方便 ;用荧光素和异硫氰酸荧光素衍生的氨基酸考察了体系性能 ,最小检测浓度为0.18μmol/L(S/N=5) ,在2×10-7~4×10-5 mol/L范围内表现出较好的线性关系(r=0.993) ,结果表明该系统灵敏度达到了普通荧光检测器的指标 相似文献
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采用自行设计、组装的毛细管电泳光导纤维发光二极管诱导荧光检测装置,建立了一种直接测定免疫球蛋白G(IgG)的方法。以蓝色发光二极管(LED)为荧光检测器的激发光源,荧光素异硫氰酸酯(FITC)为柱前衍生试剂,采用毛细管区带电泳,以20 mmol/L硼砂缓冲溶液(pH9.2)为背景电解液进行分离检测。通过对衍生反应条件和电泳分离条件进行优化,确定了最佳实验条件,在该条件下,IgG的线性范围为4.5×10-8~1.2×10-6g/L,检出限为2.0×10-8g/L。该方法简单、高效、选择性好,无需前处理,可用于人血清中IgG含量的测定。 相似文献
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报道了一种测定大黄酸的快速毛细管电泳电化学方法。采用简易制作的一种双通道-双工作电极电化学系统,可以实现电导法和安培法同时检测;优化选择了缓冲介质、工作电极、检测电位、毛细管长度和内径以及分离电压等实验参数,并对提高分析速度进行了初步研究和探讨。结果表明:大黄酸在100s内可以得到较好的分离测定,电导法和安培法的线性范围分别为6.83×10-4~1.07×10-5mol/L和3.41×10-4~2.67×10-6mol/L,最小检出浓度分别为5.28×10-6mol/L和3.16×10-7mol/L。采用设计的双通道 双工作电极检测装置,可以充分发挥电导法和安培法的优越性,对样品峰及样品的纯度进行确证;另外通过采用较短的毛细管与适当提高分离电压,可以提高分析速度。该法已用于中药大黄中大黄酸的测定。 相似文献
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用芯片毛细管电泳激光诱导荧光检测系统研究了分离多种荧光素异硫氰酸酯(FITC)衍生氨基酸的实验条件.采用以乙醇为有机添加剂的胶束毛细管电动色谱分离体系(50 mmol/LSDS,体积分数为15%的乙醇,5 mmol/L pH 9.2的硼砂缓冲液),在72 mm长的通道上实现了10种常见氨基酸的分离,一次分离的时间小于5 min. 相似文献
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Eiichi Sato Yasuyuki Oda Abulajiang Abudurexiti Osahiko Hagiwara Hiroshi Matsukiyo Akihiro Osawa Toshiyuki Enomoto Manabu Watanabe Shinya Kusachi Shigeaki Sugimura Haruyuki Endo Shigehiro Sato Akira Ogawa Jun Onagawa 《Radiation Physics and Chemistry》2011,80(12):1327-1332
6 Mcps photon counting was carried out using a detector consisting of a 1.0 mm-thick LSO [Lu2(SiO4)O] single-crystal scintillator and an MPPC (multipixel photon counter) module in an X-ray computed tomography (CT) system. The maximum count rate was 6 Mcps (mega counts per second) at a tube voltage of 100 kV and a tube current of 0.91 mA. Next, a photon-counting X-ray CT system consists of an X-ray generator, a turntable, a scan stage, a two-stage controller, the LSO–MPPC detector, a counter card (CC), and a personal computer (PC). Tomography is accomplished by repeated linear scans and rotations of an object, and projection curves of the object are obtained by the linear scan with a scan velocity of 25 mm/s. The pulses of the event signal from the module are counted by the CC in conjunction with the PC. The exposure time for obtaining a tomogram was 600 s at a scan step of 0.5 mm and a rotation step of 1.0°, and photon-counting CT was accomplished using gadolinium-based contrast media. 相似文献
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First separation of four aromatic acids and two analogues with similar structures and polarities from Clematis akebioides by high‐speed counter‐current chromatography 下载免费PDF全文
Chengcheng Gong Tao Chen Huifeng Chen Shanshan Zhang Xinzhu Wang Weiqing Wang Jing Sun Yulin Li Zhixin Liao 《Journal of separation science》2016,39(23):4660-4666
In this paper, we report an efficient method by high‐speed counter‐current chromatography for the first separation of four aromatic acids and two analogs with similar structures and polarities from Clematis akebioides. First, the ethyl acetate extract was treated by silica gel column chromatography to enrich the target compounds. And then the fraction with target compounds were purified by high‐speed counter‐counter chromatography using a two‐phase solvent system consisting of chloroform/acetonitrile/water (10:6:4, v/v). The results showed high‐speed counter‐current chromatography could be a powerful technology for the separation of compounds with similar structures and polarities. Besides, it was found acetonitrile could be a good methanol substitute when a chloroform/methanol/water system could not provide a good separation factor. This study provides a reference for the separation of compounds from Clematis akebioides. 相似文献
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The photon counting histogram (PCH) analysis is a fluorescence fluctuation method that is able to characterize the brightness and concentration of different fluorescent species present in a liquid sample. We find that the PCH model using a three-dimensional Gaussian observation volume profile is inadequate for fitting experimental data obtained from a confocal setup with one-photon excitation. We propose an imoroved model, which is based on the correction to the observation volume profile for the out-of-focus emission. We demonstrate that this model is able to resolve different species present under a wide range of conditions. Attention is given to how this model allows the examination of the effects of different instrumental setups on the resolvability. 相似文献
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《Analytical letters》2012,45(7):445-450
Abstract Tellurium has been determined at milligram and submilligram levels in the presence of uranium by a strictly instrumental photon activation 121 analysis procedure. The 575 keV gamma ray of Te was measured. At a decay time of three weeks, this gamma ray peak was interference-free. As little as 0.4 mg of tellurium was determined and the method evidences that sensitivity would increase with irradiation time. 相似文献
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Wen Su Qi Liu Qing Yang Jingang Yu Xiaoqing Chen 《Journal of separation science》2013,36(20):3338-3344
An off‐line 2D high‐speed counter‐current chromatography technique in preparative scale has been successfully applied to separate and purify the main compounds from the ethyl acetate extract of Desmodium styracifolium. A two‐phase solvent system composed of n‐hexane/ethyl acetate/methanol/water at an optimized volume ratio of 1:2:1:2 v/v/v/v was used. Conventional high‐speed counter‐current chromatography was used as the first dimension, and the upper phase of the solvent system was used as the stationary phase in the head‐to‐tail elution mode at a flow rate of 2.0 mL/min and a rotation speed of 900 rpm. Recycling high‐speed counter‐current chromatography served as the second dimension to separate an impure fraction of the first dimension. A total of four well‐separated substances including vanillic acid ( 1 ), β‐sitosterol ( 2 ), formononetin ( 3 ), and aromadendrin ( 4 ) were obtained, and their purities and structures were identified by HPLC–MS and 1H NMR spectroscopy. The results illustrated that off‐line 2D high‐speed counter‐current chromatography is an effective way to isolate compounds in complex samples. 相似文献
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光子存储原理与光致变色材料 总被引:14,自引:0,他引:14
光子存储是信息科学中的一个前沿课题。本文主要包括以下内容: 光子存储的4种方式; 光子烧孔、电子捕获、光折变和光致变色存储的原理和材料; 双光子三维光存储原理和材料; 可能的发展方向。 相似文献
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《先进技术聚合物》2018,29(1):401-406
Polypyrrole films on fluorine doped tin oxide (FTO)‐coated glass substrate were prepared in situ by placing FTO/glass substrates where pyrrole was polymerized by methyl orange‐ferric chloride complex. The atomic force microscopy image indicated growth of acicular nanorods of polypyrrole. These films exhibited catalytic activity towards I3−/I− redox couple and have been investigated for counter electrode application in dye‐sensitized solar cell (DSSC). The fabricated DSSC with N719 dye/TiO2 as photoanode, and PPy/FTO as counter electrode shows ~1.7% efficiency. 相似文献