| 毛细管电泳发光二极管诱导荧光对免疫球蛋白G的检测 |
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| 引用本文： | 甘杰,张丹,罗岳平,胡军,黄东勤.毛细管电泳发光二极管诱导荧光对免疫球蛋白G的检测[J].分析测试学报,2010,29(3). |
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| 作者姓名： | 甘杰 张丹 罗岳平 胡军 黄东勤 |
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| 作者单位： | 1. 湖南省环境监测中心站,湖南,长沙,410014
2. 湖南美可达生物资源有限公司,湖南,长沙,410016 |
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| 摘 要： | 采用自行设计、组装的毛细管电泳光导纤维发光二极管诱导荧光检测装置,建立了一种直接测定免疫球蛋白G(IgG)的方法。以蓝色发光二极管(LED)为荧光检测器的激发光源,荧光素异硫氰酸酯(FITC)为柱前衍生试剂,采用毛细管区带电泳,以20 mmol/L硼砂缓冲溶液(pH9.2)为背景电解液进行分离检测。通过对衍生反应条件和电泳分离条件进行优化,确定了最佳实验条件,在该条件下,IgG的线性范围为4.5×10-8~1.2×10-6g/L,检出限为2.0×10-8g/L。该方法简单、高效、选择性好,无需前处理,可用于人血清中IgG含量的测定。
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| 关 键 词： | 免疫球蛋白G 毛细管电泳 发光二极管 荧光检测 |
Determination of IgG by Capillary Electrophoresis with Optical Fiber Light-emitting Diode Induced Fluorescence Detection |
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| GAN Jie,ZHANG Dan,LUO Yue-ping,HU Jun,HUANG Dong-qin.Determination of IgG by Capillary Electrophoresis with Optical Fiber Light-emitting Diode Induced Fluorescence Detection[J].Journal of Instrumental Analysis,2010,29(3). |
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| Authors: | GAN Jie ZHANG Dan LUO Yue-ping HU Jun HUANG Dong-qin |
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| Institution: | GAN Jie1,ZHANG Dan2,LUO Yue-ping1,HU Jun1,HUANG Dong-qin1(1.Hunan Environmental Monitoring Centre,Changsha 410014,China,2.Hunan Micolta Bioresource Inc,Changsha 410016,China) |
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| Abstract: | A new method for direct determination of IgG without purification was developed by capillary electrophoresis(CE) with optical fiber light-emitting diode(LED) induced fluorescence detection.Fluorescence isothiocyanate(FITC) was selected as precolumn derivatization reagent in capillary zone electrophoresis.The conditions of derivatization and separation were optimized.The result indicated that the best derivatization conditions was used buffer of 10 mmol/L borate,reaction time of 16 h at room temperature,and ... |
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| Keywords: | IgG capillary electrophoresis LED fluorescence detection |
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