荧光分光光度法测定水中痕量钴和镍

试验合成了一种荧光试剂对-氨-苯基卟啉(TAPP),该试剂与钴(Ⅱ)离子和镍(Ⅱ)离子反应发生荧光淬灭,据此试验了以其为探针测定痕量钴(Ⅱ)和镍(Ⅱ)的荧光分光光度法。在pH7.2的Tris-盐酸缓冲介质中,在一定量的十二烷基硫酸钠(SDS)存在下,通过测定样品溶液和空白溶液在激发波长392 nm和发射波长516 nm处的发射荧光强度F_s及F_0,计算得反应液荧光强度减弱程度△F,其结果表明:△F与钴(Ⅱ)和镍(Ⅱ)的浓度均在2.0×10~(-7)～1.0×10~(-5)mol·L~(-1)范围内呈线性关系,方法的检出限(3S/N)分别为2.0×10~(-8)mol·L~(-1)和4.0×10~(-8)mol·L~(-1)。此方法用于自然水体和污水中钴(Ⅱ)和镍(Ⅱ)的测定,回收率分别在97.0%～108.5%和97.0%～106.5%之间。     

含氨基酸席夫碱的5-氟尿嘧啶类衍生物在银溶胶上的吸附状态研究

合成了三种含氨基酸席夫碱的5-氟尿嘧啶类衍生物,用元素分析和1H NMR表征了它们的结构;用拉曼光谱（RS）和表面增强拉曼光谱（SERS）研究目标化合物在银溶胶上的吸附状态,推测其SERS增强机理.结果表明：在银溶胶表面上,羧基氧原子与银形成化学建,苯环是稍微倾斜地吸附在银胶面上,其它部分则平躺吸附于银溶胶表面.     

早期自然流产患者绒毛滋养细胞中CXCL14的表达及其临床意义

目的研究正常早孕妇女和早期自然流产妇女之间绒毛滋养细胞中CXCL14的表达情况，探讨其表达的临床意义。方法选取33例正常早孕妇女绒毛组织和37例早期自然流产妇女绒毛组织，HE染色后光镜下观察两组绒毛组织的形态学变化；采用免疫组化SP三步法检测两组绒毛滋养细胞中CXCL14的表达。结果HE染色下两组绒毛滋养细胞形态对比，见早期自然流产组绒毛组织滋养细胞层变薄、滋养细胞变性甚至坏死、滋养细胞嗜酸性增强、绒毛间质水肿坏死；免疫组化示正常早孕组绒毛滋养细胞CXCL14的表达量为0.07±0.05，自然流产组绒毛滋养细胞CXCL14的表达量为0.12±0.02，两组比较差异具有统计学意义（P＜0.01）；首次流产组CXCL14表达量0.116±0.067，多次流产组CXCL14表达量0.086±0.127，两组比较差异无统计学意义（P＞0.01）。结论早期自然流产绒毛滋养细胞中CXCL14表达升高，可能在自然流产的发生中起着重要作用。     

含氨基酸席夫碱的5-氟尿嘧啶类衍生物的合成及Raman光谱分析

以N1-(2-四氢呋喃烷基)-5-氟尿嘧啶为原料,合成了三种含氨基酸席夫碱的5-氟尿嘧啶类衍生物,用元素分析,1HNMR表征了它们的结构。用拉曼光谱(RS)和表面增强拉曼光谱(SERS)分析了目标化合物的分子振动形式,比较它们在RS和SERS中的异同点。     

4-氟苯甲醛酪氨酸席夫碱的制备及其振动光谱的密度泛函理论和从头算理论计算的研究

4-氟苯甲醛酪氨酸席夫碱(4-FT)由4-氟苯甲醛和L-酪氨酸钾盐在室温条件下反应制得,4-FT的结构通过1HNMR,FTIR和Raman测试得到确认。分别用密度泛函(DFT,density functional theory)理论的B3LYP(Becke’s three-parameter exchange functional(B3)with Lee,Yang,Parr(LYP))方法和从头算(ab initio)理论的HF(Hartree-Fock)方法,选用标准的6-31G**和6-31G*基组对化合物4-FT的几何构型进行优化。同时分别在B3LYP/6-31G**,B3LYP/6-31G*,HF/6-31G**和HF/6-31G*水平下计算4-FT的振动波数。用密度泛函(DFT)的B3LYP方法计算并校正后的波长数据比用从头算(ab i-nitio)的HF方法计算的结果更与实验数据吻合。     

4-氟苯甲醛丙氨酸席夫碱的合成及其IR和Raman光谱分析

席夫碱及其金属配合物具有杀菌、抗菌、植物生长调节等生理活性,在医药、农药等领域有广泛的应用前景[1-2].含氟席夫碱及其配合物具有独特的生物活性, 在医药研究中备受关注[3-4]; 氨基酸席夫碱一直是人们感兴趣的研究课题,但对含氟的氨基酸席夫碱的研究未见报道.本文报道了4-氟苯甲醛与丙氨酸形成的席夫碱的合成方法及利用1H NMR、红外光谱、拉曼光谱、热重分析等测试手段进行表征的结果,化合物的生物活性研究正在进行.     

Quantitative damage imaging using Lamb wave diffraction tomography

In this paper, we investigate the diffraction tomography for quantitative imaging damages of partly through-thickness holes with various shapes in isotropic plates by using converted and non-converted scattered Lamb waves generated numerically. Finite element simulations are carried out to provide the scattered wave data. The validity of the finite element model is confirmed by the comparison of scattering directivity pattern(SDP) of circle blind hole damage between the finite element simulations and the analytical results. The imaging method is based on a theoretical relation between the one-dimensional(1D) Fourier transform of the scattered projection and two-dimensional(2D) spatial Fourier transform of the scattering object. A quantitative image of the damage is obtained by carrying out the 2D inverse Fourier transform of the scattering object. The proposed approach employs a circle transducer network containing forward and backward projections, which lead to so-called transmission mode(TMDT) and reflection mode diffraction tomography(RMDT),respectively. The reconstructed results of the two projections for a non-converted S0 scattered mode are investigated to illuminate the influence of the scattering field data. The results show that Lamb wave diffraction tomography using the combination of TMDT and RMDT improves the imaging effect compared with by using only the TMDT or RMDT. The scattered data of the converted A0 mode are also used to assess the performance of the diffraction tomography method. It is found that the circle and elliptical shaped damages can still be reasonably identified from the reconstructed images while the reconstructed results of other complex shaped damages like crisscross rectangles and racecourse are relatively poor.