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1.
Nolte Steffen Fink Reinhard Krooss Bernhard M. Amann-Hildenbrand Alexandra Wang Yue Wang Moran Schmatz Joyce Klaver Jop Littke Ralf 《Transport in Porous Media》2021,137(3):519-553
Transport in Porous Media - To improve the understanding of gas transport processes in tight rocks (e.g., shales), systematic flow tests with different gases were conducted on artificial micro- to... 相似文献
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The minor relation of functions is generalized to multisorted functions. Pippenger’s Galois theory for minor-closed sets of functions is extended to multisorted functions and multisorted relation pairs. Reflections of minor-closed sets are again minor-closed, and the effect of reflections on the invariant relation pairs of minor-closed sets of multisorted functions is described. 相似文献
3.
Reinhard Diestel 《Order》2018,35(1):157-170
Abstract separation systems provide a simple general framework in which both tree-shape and high cohesion of many combinatorial structures can be expressed, and their duality proved. Applications range from tangle-type duality and tree structure theorems in graphs, matroids or CW-complexes to, potentially, image segmentation and cluster analysis. This paper is intended as a concise common reference for the basic definitions and facts about abstract separation systems in these and any future papers using this framework. 相似文献
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Cover Picture: Robust Chemical Preservation of Digital Information on DNA in Silica with Error‐Correcting Codes (Angew. Chem. Int. Ed. 8/2015) 下载免费PDF全文
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Yuanzhong Yang Reinhard I. BoysenJamil Chowdhury Asif AlamMilton T.W. Hearn 《Analytica chimica acta》2015
In this study, the advantages of carrying out the analysis of peptides and tryptic digests of proteins under gradient elution conditions at pH 6.5 by reversed-phase liquid chromatography (RP-HPLC) and in-line electrospray ionisation mass spectrometry (ESI-MS) are documented. For these RP separations, a double endcapped, bidentate anchored n-octadecyl wide pore silica adsorbent was employed in a capillary column format. Compared to the corresponding analysis of the same peptides and protein tryptic digests using low pH elution conditions for their RP-HPLC separation, this alternative approach provides improved selectivity and more efficient separation of these analytes, thus allowing a more sensitive identification of proteins at different abundance levels, i.e. more tryptic peptides from the same protein could be confidently identified, enabling higher sequence coverage of the protein to be obtained. This approach was further evaluated with very complex tryptic digests derived from a human plasma protein sample using an online two-dimensional (2D) strong cation-exchange (SCX)-RP-HPLC-ESI-MS/MS system. Again, at pH 6.5, with mobile phases of different compositions, improved chromatographic selectivities were obtained, concomitant with more sensitive on-line electrospray ionisation tandem mass spectrometric (ESI-MS/MS) analysis. As a consequence, more plasma proteins could be confidently identified, highlighting the potential of these RP-HPLC methods with elution at pH 6.5 to extend further the scope of proteomic investigations. 相似文献
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Dendritic Glycopolymer as Drug Delivery System for Proteasome Inhibitor Bortezomib in a Calcium Phosphate Bone Cement: First Steps Toward a Local Therapy of Osteolytic Bone Lesions 下载免费PDF全文
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In this investigation, methods based on on-probe enzymatic cleavage matrix-assisted laser desorption/ionization time-of-flight mass spectrometric (MALDI-TOF-MS) analyses have been developed, allowing the rapid assignment of phosphorylation sites within phosphoproteins. The procedures involved robotic sample deposition of a phosphoprotein, such as intact bovine β-casein, on stainless steel or gold MALDI plates, on-probe proteolysis with trypsin for 10–180?s at 37°C, on-probe dephosphorylation for 1–10?min at 37°C with alkaline phosphatase, followed by differential mass spectrometry with peptide mass mapping. The dephosphorylation conditions were initially optimized using in-solution tryptic digestion of the phosphoprotein performed in the presence of MS-compatible anionic surfactant sodium 3-[(2-methyl-2-undecyl-1,3-dioxolan-4-yl)methoxy]-1-propanesulfonate. Two methods of trypsin deactivation were investigated, cooling and quenching by acidification, which resulted in the surfactant either staying intact or becoming cleaved, respectively. Since the surfactant had no detrimental effects on dephosphorylation of phosphopeptides, the acidification and neutralization steps were not included in the final analytical method. A protocol, comprising on-probe tandem, surfactant-aided proteolysis for 3?min followed by on-probe dephosphorylation for 10?min was thus established, allowing the rapid identification of location and sequence of phosphopeptides within a phosphoprotein by these procedures. 相似文献