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糖蛋白激素的不均一致及其色谱分离纯化研究的进展 总被引:1,自引:0,他引:1
本文介绍了糖蛋白激素的不均一性,讨论了影响其色谱分离纯化过程的各种因素,以及色谱分离模式与操作模式的选择。 相似文献
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本文介绍了糖蛋白激素的不均一性, 讨论了影响其色谱分离纯化过程的各种因素, 以及色谱分离模式与操作模式的选择。 相似文献
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小鼠血清IgG的蛋白G灌注亲和色谱行为的研究 总被引:3,自引:0,他引:3
通过实验考察了小鼠血清IgG的蛋白G灌注亲和色谱行为,虽然未发现非特异性吸附,但在通常情况下对IgG存在不可逆性吸附。洗脱液组成及pH的不同决定IgG在灌注亲和色谱柱上具有不同的解离常数KD,产生不同的色谱行为。流动相的流速对IgG与蛋白G的结合/解离动力学过程产生一定的影响。结果表明,对于小鼠血清中的IgG灌注亲和色谱的分离,由于解离速率影响到传质过程,使之在高流速下的分离受到一定的限制。 相似文献
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A series of LiNbO_3 crystals tri-doped with Mg~(2+),Yb~(3+),and Ho~(3+) are grown by the conventional Czochraski technique.The concentrations of Mg~(2+),Yb~(3+),and Ho~(3+) ions in Mg:Yb:Ho:LiNbO_3 crystals are measured by using an inductively coupled plasma atomic emission spectrometry.The x-ray diffraction is proposed to determine the lattice constant and analyze the internal structure of the crystal.The light-induced scattering of Mg:Yb:Ho:LiNbO_3 crystal is quantitatively described via the threshold effect of incident exposure energy flux.The exposure energy(E_r) is calculated to discuss the optical damage resistance ability.The exposure energy of Mg(7 mol):Yb:Ho:LiNbO3 crystal is 709.17 J/cm~2,approximately 425 times higher than that of the Mg(l mol):Yb:Ho:LiNbO_3 crystal in magnitude.The blue,red,and very intense green bands of Mg:Yb:Ho:LiNbO_3 crystal are observed under the 980-nm laser excitation to evaluate the up-conversion emission properties.The dependence of the emission intensity on pumping power indicates that the up-conversion emission is a two-photon process.The up-conversion emission mechanism is discussed in detail.This study indicates that Mg:Yb:Ho:LiNbO_3 crystal can be applied to the fabrication of new multifunctional photoluminescence devices. 相似文献
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