| 促卵泡激素的径向色谱纯化工艺 |
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| 引用本文： | 郭立安,阎哲,杨晓东,褚西秦,徐海峰.促卵泡激素的径向色谱纯化工艺[J].色谱,2000,18(6):577-579. |
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| 作者姓名： | 郭立安 阎哲 杨晓东 褚西秦 徐海峰 |
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| 作者单位： | 1. 第四军医大学中心实验室,陕西,西安,710032
2. 第四军医大学第二附属医院核医学科,陕西,西安,710038 |
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| 基金项目： | 陕西省科学技术发展计划项目攻关课题! (95K11 8)，高等学校骨干教师资助计划资助 |
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| 摘 要： | 比较了径向离子交换色谱与经典离子交换色谱在纯化工艺放大过程中的分离效果 ,证实了径向色谱比经典色谱更适合于促卵泡激素 (FSH)的纯化 ,并建立了适合于大规模生产FSH的径向色谱纯化新工艺。纯化后FSH的比活性为 180IU/mg ,活性回收率为 5 6 % ,产品质量符合国家药典规定。
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| 关 键 词： | 径向色谱 离子交换色谱 促卵泡激素 纯化 |
Purification of Follicle-Stimulating Hormone by Radial-Flow Chromatography |
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| GUO Li-an,YAN Zhe,YANG Xiao-dong,CHU Xi-qin,XU Hai-feng.Purification of Follicle-Stimulating Hormone by Radial-Flow Chromatography[J].Chinese Journal of Chromatography,2000,18(6):577-579. |
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| Authors: | GUO Li-an YAN Zhe YANG Xiao-dong CHU Xi-qin XU Hai-feng |
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| Institution: | Central Labaratory, 4th Military Medical University, Xi'an 710032, China. lianguo@fmmu.edu.cn |
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| Abstract: | By comparing the purification results of radial flow chromatography with conventional chromatography, a new purification process of follicle stimulating hormone (FSH) from crude sample of human menopausal gonadotrophin has been developed The results showed that the specific activity, activity recovery, and purification factor of FSH purified by radial flow chromatography were higher than those by conventional chromatography in large scale The specific activity of FSH purified was 180 IU/mg protein, activity recovery 56%, purification factor 150 The purified product meets the quality requirement of the Standard of Chinese Pharmacopoeia. |
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| Keywords: | radial flow chromatography ion exchange chromatography follicle stimulating hormone purification |
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