用于单细胞RNA分析的循环肿瘤细胞团捕获和单细胞分离方法的质量评估

循环肿瘤细胞团（Circulating tumor cell cluster, CTC cluster）的单细胞分析对CTC cluster的生物学效应分析极其重要。为了使CTC cluster单细胞分析可以获得最有效的结果,分析前的捕获和单细胞分离的过程必须能够保持CTC cluster的完整性和细胞内部RNA的完整。本研究通过单细胞RNA测序中的逆转录方法逆转录并定量3个看家基因进行RNA质控,同时测量CTC cluster的横向直径用于评估CTC cluster的完整性,从而测定CTC cluster捕获和单细胞分离过程对CTC cluster单细胞分析结果的影响。分别采用胰酶-EDTA和非酶细胞解离溶液（Non-enzymatic cell dissociation solution, NECDS）分离CTC cluster中的单细胞,并考察了单细胞分离过程对胞内RNA的影响。考察了负向富集、正向富集和膜过滤捕获方案中不同操作流程对CTC cluster完整性和单细胞内RNA完整性的影响。结果表明,NECDS可快速分离CTC cluster中的单细胞且不会对细胞内RNA产生影响...     

STUDIES ON THE REGULAR COPOLYMERS OF POLY (1, 4-PHENYLENETEREPHTHALAMIDE)

Two types of the regular copolymer of poly (1, 4-phenyleneterephthalamide) were synthesized by the low temperature solution polycondensation in NMP-CaCl_2 solvent system, using the piperazine or 2,5-dimethylpiperazine as the third components introduced in the main chain of poly (1, 4-phenyleneterephthalamide). The properties of copolymers were characterized by IR, SEM, X-RAY diffraction, polarizing microscopy, TGA and solubilities.Experimental results showed that the copolymers had good solubility and thermal stability, the concentrated sulfuric acid (～98% ) solution of regular PPTA copolymers had liquid crystalline proper-     

THE POLYCONDENSATION OF TEREPHTHALOYL CHLORIDE AND p-PHENYLENEDIAMINE HYDROCHLORIDE IN NMP-CaCl_2 SOLVENT SYSTEM

The solution polycondensation of 1,4-phenylenediamine hydrochloride and terephthaloyl chlo-ride with introducing a tertiary amine in NMP-CaCl_2 solvent system were studied in detail.Effect of reactant concentration, the content of CaCl_2, in NMP solvent and the kinds of tertiary amineon inherent viscosity of PPTA polymer have been studied. High molecular weight PPTA withinherent viscosity over 5 was obtained at 0.35Mol/L of reactant concentration in a solvent of NMP-CaCl_2 with the presence of α-picoline.     

气相色谱法直接进样测定饮用水中挥发性卤代烃

 利用大口径毛细管柱气相色谱/电子捕获检测器(GC/ECD)方法,直接进样测定了饮用水中的三氯甲烷、四氯化碳、一溴二氯甲烷、二溴一氯甲烷、三溴甲烷等5种挥发性卤代烃。所建立的方法简便、快速、灵敏度高,并具有较好的精密度与准确度。当5种卤代烃化合物的质量浓度平均值为9.03～20.19μg/L时,CV为2.9%～7.5%,标准差为0.3～0.9μg/L。样品的加标回收率为80%～118%,标准曲线的相关系数为0.998和0.999。ECD的温度为350℃时,其最低检测限在0.1～0.7μg/L范围内,均低于文献报道值 。     

关于Birch还原反应产物的探讨

Birch还原反应产物为非共轭的烯烃，其原因在各种基础有机化学教材中都未有明确探讨。现有机理表明，决定产物双键位置的一步是芳香族化合物获得2个电子后形成的负离子中间体的质子化。基于量子化学计算与其他文献的报道对质子化的区域选择性做出了探讨。结论从定性与定量2方面指出，上述质子化的反应位点是中间体HOMO中轨道系数最大的碳原子，即该反应的区域选择性由动力学因素控制。     

STUDIES ON THE POLYPYRROLE/POLYELECTROLYTE MOLECULAR COMPOSITES

The potyelectrolyte of propane sulfonate(PS) grafted PPTA copolymers——PPTA-PS, PPTA[O]-PS, PPTA[C]-PS were prepared and used as electrolyte in the process of electrochemical polymerization of pyrrole to form the molecular composite polypyrrole (PPY)/Polyelectrolyte.The preparation and liquid crystalline property of three kinds of polyelectrolyte, the electrical conductivity, mechanical properties, SEM and thermoproperties of PPY/polyelectrolyte are presented in detail.     

对苯二胺盐酸盐与对苯二甲酰氯在NMP-CaCl_2溶剂体系中的缩聚反应

<正> 聚对苯二甲酰对苯二胺(PPTA)具有刚性链分子结构,能形成各向异性的高分子液晶溶液,纺丝后可得到性能优异的高模量、高强度的纤维。但是,欲制出高模高强的PPTA纤维,必须先制出高分子量的PPTA聚合体。等曾研究了对苯二胺盐酸盐与对苯二甲酰氯在二甲基乙酰胺-氯化锂(DMAC-LiCl)溶剂体系中合成PPTA的问题。     

两种萘酰亚胺类化合物及其聚合物荧光性质的研究

本文以1,8萘酰亚胺为原料,合成了两种萘酰亚胺类型的化合物,系统地研究了它们在不同溶剂中的吸收光谱和荧光光谱.并以这两种化合物单体为基础,通过共聚合成了它们与甲基丙烯酸甲酯和N 乙烯基咔唑的嵌段共聚物.研究了聚合物在溶液和薄膜中的荧光性质.研究结果证明,所得共聚物不但保持了单体的基本荧光特性,其溶解性、成膜性、热稳定性等都得到了大大改善,是一种有应用前景的有机发光半导体材料.此外,本文还利用荧光猝灭的手段研究了萘酰亚胺类化合物与C₆₀之间的相互作用.