MODULATING ACTIVITY OF B LYMPHOCYTES ON HAEMOPOIESIS (Ⅱ)——THE PURIFICATION OF PROLIFERATION AMPLIFYING FACTOR (PAF)

In recent years we have done a series of observations on the haemopoietic modulation effects oflymphocytes. Now we report here, for the first time, the pulification of a modulating factor (prolif-eration amplifying factor, PAE) with affinity chromatography and HPLC from supernatant conditionedby a lymphoma cell line M12.4.1. RAF exhibits on SDS-PAGE only one band, the molecular weightis about 31,000, the specific activity is 20,000 times that in the conditioned medium. It may providea direct evidence that the B lymphocyte also possesses some haemopoietic modulation effects.     

B淋巴细胞造血调控活性的研究——Ⅱ.增殖放大因子的分离提纯

本文首次报道用亲和层析和高效液相色谱等技术由B淋巴瘤M12.4.1的培养上清中分离到一种新的造血调控因子——增殖放大因子(PAF)。PAF在SDSPAGE电泳上呈现分子量为31000 D的单一谱带,比活性较M12.4.1培养上清提高了20000倍,为B淋巴细胞的造血调控作用提供了直接的证据。