石榴状凝胶微球固定化漆酶的制备、 表征及降解双酚A

采用铜/锌复合金属磷酸盐晶体和海藻酸钙凝胶双重包覆技术对漆酶进行固定化, 制得石榴状Alg@Cu₃/Zn₃(PO₄)₂@Lac的凝胶微球. SEM, EDX和FTIR表征结果表明, 在凝胶微球内部, 漆酶被成功固定于由海藻酸钙凝胶包覆的铜/锌复合金属磷酸盐晶体内, 铜/锌复合金属磷酸盐晶体镶嵌于海藻酸钙凝胶网格的孔隙中而呈石榴状. 以2,2′-联氮双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)为底物, 经酶学性质研究表明, 在无机盐晶体和海藻酸钙凝胶的双重保护下, Alg@Cu₃/Zn₃(PO₄)₂@Lac的耐热性、 耐酸性以及储存稳定性比游离漆酶均有不同程度增强. 将Alg@Cu₃/Zn₃(PO₄)₂@Lac应用于双酚A(BPA)的降解, 采用孔径约1 mm滤网实现快速回收, 经6次循环利用, 对BPA的降解率下降约14%, 显示出比较稳定的重复利用性和便捷的可操作性, 这主要得益于海藻酸钙和铜/锌无机盐晶体对漆酶蛋白分子的双重保护.     

由三个特殊次序向量对构造三对角对称矩阵

讨论利用给定的三个特殊次序向量对构造不可约三对角矩阵、Jacobi矩阵和负Jacobi矩阵的反问题.在求解方法中,将已知的一些关系式等价地转化为线性方程组,利用线性方程组有解的条件,得到了所研究问题有惟一解的充要条件,并给出了数值算法和例子.     

Molecular Dynamic Simulation on the Absorbing Process of Isolating and Coating of α-olefin Drag Reducing Polymer

The absorbing process in isolating and coating process of α-olefin drag reducing polymer was studied by molecular dynamic simulation method, on basis of coating theory of α-olefin drag reducing polymer particles with polyurethane as coating material. The distributions of sodium laurate, sodium dodecyl sulfate, and sodium dodecyl benzene sulfonate on the surface of α-olefin drag reducing polymer particles were almost the same, but the bending degrees of them were obviously different. The bending degree of SLA molecules was greater than those of the other two surfactant molecules. Simulation results of absorbing and accu-mulating structure showed that, though hydrophobic properties of surfactant molecules were almost the same, water density around long chain sulfonate sodium was bigger than that around alkyl sulfate sodium. This property goes against useful absorbing and accumulating on the surface of α-olefin drag reducing polymer particles; simulation results of interactions of different surfactant and multiple hydroxyl compounds on surface of particles showed that, interactions of different surfactant and one kind of multiple hydroxyl compound were similar to those of one kind of surfactant and different multiple hydroxyl compounds. These two contrast types of interactions also exhibited the differences of absorbing distribution and closing degrees to surface of particles. The sequence of closing degrees was derived from sim-ulation; control step of addition polymerization interaction in coating process was absorbing mass transfer process, so the more closed to surface of particle the multiple hydroxyl com-pounds were, the easier interactions with isocyanate were. Simulation results represented the compatibility relationship between surfactant and multiple hydroxyl compounds. The isolating and coating processes of α-olefin drag reducing polymer were further understood on molecule and atom level through above simulation research, and based on the simulation, a referenced theoretical basis was provided for practical optimal selection and experimental preparation of α-olefin drag reducing polymer particles suspension isolation agent.     

斜率巧双用 旧题焕新解

<正>斜率关系是圆锥曲线问题中常见的一种关系,常见的有斜率和、积、商等,涉及定点定值问题．处理这种关系,通常是设线用韦达定理或结合斜率齐次化处理．本文给出这类问题的另一种处理方法“斜率双用”,再以高考真题阐述这一方法的具体应用,帮助同学们理解和掌握这种方法的原理和应用技巧,拓宽解题思维,提升数学运算能力．     

双氰基荧光染料的合成、光学性质及其生物成像

设计合成了一种A-π-D-π-A型的双光子荧光染料3,6-双(4-乙烯基苯腈)-9-乙基咔唑,测试了其在二氯甲烷(DCM)、乙酸乙酯(EA)、乙醇(Et OH)、乙腈(ACN)、二甲亚砜(DMSO)和磷酸缓冲盐溶液(PBS)等不同溶剂中的紫外吸收光谱、单光子及双光子荧光光谱。化合物3,6-双(4-乙烯基苯腈)-9-乙基咔唑在紫外吸收光谱中存在两个相似的特征吸收带并呈现出复杂的溶剂化效应,在DMSO中具有最大荧光量子产率(86.02%),其相应的活性吸收截面为12.56 GM。在双光子荧光成像方面,染料分子具有优良的细胞膜通透性并且在双光子荧光显微镜下呈现出明亮的绿色荧光,表现出较好的双光子荧光成像性能。这些数据表明,化合物3,6-双(4-乙烯基苯腈)-9-乙基咔唑可用作一种较为理想的双光子荧光标记染料。     

镁基复合材料的放氢性能

将混合粉末Ni和Ti覆盖于纯Mg锭表面制备Mg基复合材料.此方法有利于吸/放氢相Mg2Ni和催化相NiTi规则地生成与分布.纯Mg锭位于中心位置,而粉末Ni和Ti包覆于纯Mg锭表面.首先确定生成Mg2Ni和NiTi的温度范围为600℃左右;根据此温度范围,计算在650℃和850℃ Mg2Ni和NiTi的晶粒尺寸,从而确定最优化的烧结温度;建立晶体结构模型,确定Mg2Ni合金相主要分布在烧结体表面;最终实现了5 min放氢量为4wt;,实现了短时间内大放氢量的目的.     

反射波对预混气体爆炸过程与管壁动态响应的影响

为研究管道内甲烷/空气混合气体火焰和压力波的传播规律,对内载压力波作用下管壁的动态响应进行实验。结果表明,末端闭口实验中,管道末端的反射激波会引起当地火焰亮度的增大,而前端反射激波则有可能导致火焰内部的分离从而出现熄灭与复燃现象。相对于末端开口工况,末端闭口实验时管道两端产生的往复反射激波对管壁具有叠加加载作用,导致管壁产生较大的环向应变。     

基于微悬臂梁技术对固液界面间横向摩擦力的检测

研究固液界面上的力学行为对于界面科学和工程应用具有重要意义,如何检测液滴在材料表面的横向摩擦力一直是界面领域研究的难题之一.本文基于微悬臂梁传感技术和视觉图像分析技术,设计并搭建了一套固液横向摩擦力检测系统,将毛细管端部的弯曲程度与固液界面的相互作用力建立关系,实现对固液界面间横向摩擦力的精确测量;通过对水滴在疏水界面...     

促性腺激素释放激素类似物-紫杉醇靶向抗肿瘤缀合物的合成及评价

以促性腺激素释放激素类似物(GnRHa)为靶向配体, 以紫杉醇为抗癌因子, 分别以硫醚键和二硫键为连接臂, 设计合成了2个靶向抗肿瘤缀合物. 研究了缀合物的肿瘤细胞增殖抑制活性和GnRH受体结合活性, 结果表明, 2个缀合物均具有较强的抗肿瘤活性和GnRH受体亲和力; 另外, 血浆稳定性实验结果显示, 以硫醚键偶联的缀合物1在血浆中孵育24 h, 原型保留仍在50%以上, 具有较高的稳定性.     

高铁酸钾的合成与电化学性能研究

本文主要研究了锂离子电池正极材料高铁酸钾的合成,表征和电化学性质.用次氯酸钾与硝酸铁于碱性介质中反应得到高铁酸钾粗品,重结晶后成纯度大于97%的产品,用XRD和FTIR等方法对高铁酸钾进行表征和分析.初步研究了K2FeO4/Li电池的充放电性能.