双氰基荧光染料的合成、光学性质及其生物成像

设计合成了一种A-π-D-π-A型的双光子荧光染料3,6-双(4-乙烯基苯腈)-9-乙基咔唑,测试了其在二氯甲烷(DCM)、乙酸乙酯(EA)、乙醇(Et OH)、乙腈(ACN)、二甲亚砜(DMSO)和磷酸缓冲盐溶液(PBS)等不同溶剂中的紫外吸收光谱、单光子及双光子荧光光谱。化合物3,6-双(4-乙烯基苯腈)-9-乙基咔唑在紫外吸收光谱中存在两个相似的特征吸收带并呈现出复杂的溶剂化效应,在DMSO中具有最大荧光量子产率(86.02%),其相应的活性吸收截面为12.56 GM。在双光子荧光成像方面,染料分子具有优良的细胞膜通透性并且在双光子荧光显微镜下呈现出明亮的绿色荧光,表现出较好的双光子荧光成像性能。这些数据表明,化合物3,6-双(4-乙烯基苯腈)-9-乙基咔唑可用作一种较为理想的双光子荧光标记染料。

Synthesis,Optical Properties and Biological Imaging of A New Two-photon Fluorescence Dye with Dicyano-group

A novel two-photon fluorescence dye 4,4′-((1Z,1′Z)-(9-ethyl-9H-carbazole-3,6-diyl) bis( ethene-2,1-diyl)) dibenzonitrile was synthesized, and the linear absorption and fluorescent spectra of the dye in different solvents were investigated. The results indicate that the dye has a in-tricate solvent effect existing in UV-Vis absorption spectra, and the quantum yields and two-photon active cross-sections in DMSO are 86. 02%, 12. 56 GM, respectively. About the images, the dye exhibites excellent cell membrane permeability and optical properties. These data show that the com-pound is a promising candidate for two-photon fluorescence labels.