Trace Metal and Persistent Organochlorine Levels in Wood Bison (Bison bison athabascae) from the Mackenzie Bison Sanctuary

Abstract

Tissue samples were collected from ten healthy mature female wood bison (B. bison athabascae) for examination. Livers and kidneys were tested for toxic heavy metals and trace metals considered as essential nutrients for successful reproduction, while fat samples were analyzed for persistent chlorinated hydrocarbons. No elevated levels of toxic heavy metals (arsenic, cadmium, lead, mercury) were found and essential trace elements (copper, manganese, selenium, zinc) were within the acceptable ranges established for healthy cattle. All fat samples tested contained trace levels of α-BHC (mean value, 23 ppb) and lindane (mean value, 3 ppb).     

毛细管气相色谱法测定清补凉中六六六、DDT的残留量

建立毛细管气相色谱法测定清补凉中六六六、滴滴涕(DDT)的残留量。用丙酮提取样品中残留的有机氯农药,石油醚萃取,萃取液以浓硫酸净化。采用HP-1石英毛细管色谱柱对清补凉中8种六六六、DDT的异构体进行了检测。结果表明,该法测定下限为2.45×10-14～1.15×10-12g,加标平均回收率为84.9%～98.6%,相对标准偏差为1.6%～4.5%。     

气相色谱法测定土壤中有机氯杀虫剂

建立了利用加速溶剂萃取和GC-ECD联用技术,对土壤中六六六、滴滴涕、艾氏剂、狄氏剂、硫丹Ⅰ、硫丹Ⅱ、硫丹硫酸盐内氯甲桥奈、内氯甲桥奈醋醛、七氯、环氧七氯17有机农药进行了分析,通过对回收率、重现性及精密度的测定,其实验具有操作简便,时间短,应用范围广的优点.     

固相萃取/毛细管气相色谱法测定肉桂皮中六六六、滴滴涕农药残留量

研究了肉桂皮中六六六、滴滴涕农药残留量的测定方法。以丙酮-石油醚混合溶剂为提取剂,旋涡振荡提取,采用固相萃取技术对提取液净化,优化洗脱溶剂的比例和用量,用气相色谱/微电子捕获检测器（μECD）测定。在优化条件下,BHC及DDT农药在10~1 000 μg/L质量浓度范围内线性关系良好,相关系数为0.995 8~0.998 1。2种农药在肉桂皮中的加标回收率为 92.1%~112%,相对标准偏差（RSD）为2.8%~8.1%,方法的检出限（LODs）为5.0×10-3~2.4×10-2 mg?kg-1,定量下限（LOQs）为1.3×10-2~6.0×10-2 mg?kg-1。方法具有简单、灵敏、选择性高、重现性好等优点。     

分散液液微萃取/气相色谱-质谱法测定蜂蜜中六六六和滴滴涕类农药残留

建立了分散液液微萃取(DLLME)与气相色谱-质谱法(GC-MS)联用快速检测蜂蜜中六六六(BHC)和滴滴涕(DDT)类农药残留的分析方法.使用三氯甲烷为萃取剂,通过涡旋、离心使分析物富集到微量三氯甲烷中,采用气相色谱-质谱进行分析.实验对影响DLLME萃取效率的因素,如萃取剂种类和体积、分散剂种类和体积、萃取时间等进行了考察,同时对方法的基质效应和性能进行了评估.结果显示:由于基质效应,8种六六六和滴滴涕类农药都出现信号增强现象.8种六六六和滴滴涕类农药在2~500 μg/L范围内线性关系良好,相关系数(r²)为0.991~0.998,方法富集倍数为74~96;当试样的加标水平为20、50和100 μg/kg时,8种六六六和滴滴涕类农药的回收率为61.0%~100.1%,相对标准偏差(RSD, n=5)为2.2%~19.5%.8种六六六和滴滴涕类农药的最低检测浓度均为20 μg/kg,最小检出量皆为1.0 ng.该方法简单、快速、高效,适用于蜂蜜中六六六和滴滴涕类农药的残留检测.     

Storage of DDT and BHC in Adipose Tissue of Indian Males

Adipose tissue of 50 human subjects (10 to 60 years old) were taken from autopsy cases and analysed by gas-liquid chromatography equipped with an electron capture detector (³H +) for determination of storage levels of BHC (benzene hexa chloride), DDT (dichlorodiphenyl trichloroethane) and its metabolites DDE and DDD in human body fat. The data is reported according to age (length of exposure), dietetic habits and area of residence of the subjects. The results show relatively moderate exposure to DDT and BHC. Levels of residues were slightly higher in non-vegetarian and rural residents than those of their counterpart vegetarian and urban inhabitants. The study suggests that DDT levels in human adipose show a decreasing trend as compared to earlier report of Dale et al. ¹ of highest body burden of pesticides being present in indians.     

微波萃取-气相色谱法测定土壤中六六六、滴滴涕

用微波萃取和GC-ECD联用技术.对土壤中六六六、滴滴涕进行了分析.实验具有操作简便,分析时间短,应用范围广等优点.并且进行了回收率、重现性及精密度的测定.     

微量化提取结合毛细管柱气相色谱法测定茶叶中六六六、滴滴涕残留量的探讨

微量化提取结合毛细管柱气相色谱法测定茶叶中六六六，滴滴涕残留量，对六六六，滴滴涕8种异构体都能得到满意的分离效果，采用外标法定量测定，且用此方法检测滴滴涕4种异构体的平均回收率在92．5％－103．2％之间，相对标准偏差在1．6％－3．1％之间，检出限可达0．04μg/kg.     

气相色谱法测定花生中六六六、滴滴涕、乙草胺和毒死蜱残留

建立了毛细管柱气相色谱法同时测定花生中六六六、滴滴涕、乙草胺和毒死蜱的方法。样品用乙腈提取,固相萃取柱净化,气相色谱仪电子捕获检测器(ECD)进行测定。结果表明,10种农药含量在0.005~1.0 mg/L之间线性关系良好,相关系数均在0.995以上,加标回收率在80.5%~116.9%之间,相对标准偏差在1.2%~5.0%之间。方法适用于花生中六六六、滴滴涕、乙草胺和毒死蜱的同时测定。     

气相色谱法测定参叶茶中农药残留量

利用气相色谱法同时测定了参叶茶中六六六和滴滴涕共 8种异构体的残留量 ,方法回收率为 78.0 %～ 1 0 0 .0 % ,最低检测浓度为 <1 .5ng/g,相对标准偏差 <1 0 .0 %。