亲水作用色谱法测定组织中全基因组DNA甲基化水平

建立了亲水作用色谱(HILIC)测定组织中全基因组DNA甲基化水平的方法。采用苯酚-氯仿提取组织中的DNA,提取的DNA用88%甲酸在140 ℃下裂解,经N2吹干后,加乙腈-水(9:1, v/v)溶解,用Waters BEH HILIC柱进行分离,在277 nm波长下检测胞嘧啶(Cyt)及5-甲基胞嘧啶(5-mCyt)含量。结果表明,以乙腈-10 mmol/L甲酸铵溶液(94:6, v/v)为流动相,流速为0.5 mL/min, Cyt与5-mCyt分离较好,保留时间分别为2.6与3.1 min。胞嘧啶的线性范围为1～900 μmol/L,相关系数为0.9999; 5-甲基胞嘧啶的线性范围为1～64 μmol/L,相关系数为0.9998。胞嘧啶和5-甲基胞嘧啶的检出限为54 nmol/L(柱中为0.54 pmol),定量限为250 nmol/L(柱中为2.5 pmol);在5～900 μmol/L的添加水平下,胞嘧啶和5-甲基胞嘧啶的平均加标回收率为94.7%～100.5%,相对标准偏差小于1.48%。用该方法检测了结肠癌组织中DNA甲基化水平,结果显示该癌组织中全基因组的DNA甲基化均值为4.0%。该方法快速、简单,稳定性好,灵敏度较高,能满足全基因组DNA甲基化的检测要求。     

Bifurcation analysis in a neutral differential equation

The dynamics of a neural network model in neutral form is investigated. We prove that a sequence of Hopf bifurcations occurs at the origin as the delay increases. The direction of the Hopf bifurcations and the stability of the bifurcating periodic solutions are determined by using normal form method and center manifold theory. Global existence of periodic solutions is established using a global Hopf bifurcation result of Krawcewicz et al. and a Bendixson's criterion for higher dimensional ordinary differential equations due to Li and Muldowney.     

Completeness of the System of Root Vectors of 2 × 2 Upper Triangular Infinite-Dimensional Hamiltonian Operators in Symplectic Spaces and Applications

The authors investigate the completeness of the system of eigen or root vectors of the 2 × 2 upper triangular infinite-dimensional Hamiltonian operator H ₀. First, the geometrical multiplicity and the algebraic index of the eigenvalue of H ₀ are considered. Next, some necessary and sufficient conditions for the completeness of the system of eigen or root vectors of H ₀ are obtained. Finally, the obtained results are tested in several examples.     

无界奇异∮-自伴算子矩阵的可逆性与可逆补

刻画了2×2无界奇异∮-自伴算子矩阵的可逆性,并且得到相应的缺项算子矩阵存在可逆补的充分必要条件,最后举例验证了结果的合理性.     

电磁力诱导二次流对微流体混合效果影响的研究

设计了一种微流道中液体混合的方法, 基于电磁力的作用, 诱导微流道中二次流动 发生, 从而产生Dean涡, 使流道中流体出现往复运动及流体界面的弯曲延伸, 使不 同流体的接触面积大大地增加, 从而提高混合效率. 对3种结构的工况进行了比较. 建立了微流道混合系统的理论模型并进行了数值模拟,对流体混合过程进行了分析并对混合效率进行了评价. 进行了验证性的试验研究, 对模拟结果与试验结果进行了比较, 结果相吻合.     

Numerical and experimental nonlinear dynamics of a proportional pressure-regulating valve

A novel direct proportional pressure-regulating valve is presented in this paper, and its working principle is introduced. The pressure of feedback chamber is controlled by two orifices. The lumped parameter double-mass dynamic model considering both the spool mass and the plunger mass is established. The model consists of the subsystem models with hydraulic fluid dynamic, valve mechanic and electromagnetic. The numerical model is validated through experiments. With the model, the spool and pressure dynamics are analysed by comparing the changes of the simulation parameters. The effects of orifice diameters, lap, spring stiffness, viscous damping coefficient on the stability of spool and pressure are investigated. The results show that a fixed relationship between the orifice diameters of the valve can be achieved. A larger overlap is beneficial to improve the stability of the spool. It is aimed to propose a parametric design method for the valve optimization.

     

White light interferometry test and analysis of LiNbO3 polarizer

White light interferometer can be used to measure the amplitude extinction ratio (ER) of polarizer and coupling distribution in fiber. A LiNbO₃ polarizer coupled with a polarization maintaining fiber and a silica planar waveguide at the two ends was measured using white light interferometer. According to the principles of optical coherence domain polarimeter (OCDP) technique, the test scheme is analyzed and presented to measure the ER of LiNbO₃ polarizer with its apparatus proposed correspondingly. By analyzing the interference intensity, both the ER of LiNbO₃ polarizer and its coupling crosstalk with optical fiber and waveguide are obtained. The results illustrate that the ER of a 5 mm-long LiNbO₃ polarizer is 71 dB and the crosstalk of the coupling points are around 40 dB. The results have good agreement with analysis.     

A large volume cubic press with a pressure-generating capability up to about 10 GPa

A massive cubic press, with a maximum load of 1400 tons on every WC anvil, has been installed at the High Pressure Laboratory of Peking University. High-P experiments have been conducted to examine the performance of the conventional experimental setup and some newly developed assemblies adopting the anvil-preformed gasket system. The experimental results suggest that (1) the conventional experimental setup (assembly BJC2-0) can reach pressures up to about 6 GPa with a large cell volume of 34.33 cm³; (2) the anvil-preformed gasket system, despite decreasing the P-generating efficiency, extends the P-generating capability up to about 8 GPa at the expense of reducing the cell volume down to 8.62 cm³ (assembly BJC2-6); (3) due to the large cell volume, it is possible to make further modifications to extend the pressure range, as readily demonstrated, to about 10 GPa (assembly BJC5-7); (4) the effect of high temperature on the pressure generation of the press is not significant. It follows that this cubic press can be very useful in synthesizing materials of large volume at high pressures and to the studies such as high-P phase equilibrium, trace element partitioning and isotope fractionation in the research fields of Earth and planetary sciences.