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Jumiarti Agus Hideki Abe Yoshiharu Doi Takeharu Tsuge 《Polymer Degradation and Stability》2006,91(8):1645-1650
Polyhydroxyalkanoate (PHA) synthase (PhaC) from Wautersia eutropha was expressed in a wide range of production level in Escherichia coli XL1-Blue cells and its effects on PhaC activity, poly[(R)-3-hydroxybutyrate] [P(3HB)] production and its molecular weights were investigated. The production level of PhaC was controlled both by the amount of chemical inducer (isopropyl-β-d-thiogalactopyranoside, IPTG) added into the medium and the use of different copy number of plasmids. In a flask experiment, as PhaC production level in the cells increased, the PhaC activity also increased in the range of low PhaC concentration. However, PhaC activity did not further increase in the range of high PhaC concentration, probably due to the formation of inclusion body in the cells. The molecular weight of P(3HB) was found to decrease with increasing PhaC activity. This trend was also verified in high cell density cultivation using 10-l jar fermentor. Furthermore, we demonstrated that the use of low copy number plasmid and appropriate induction of PhaC expression were effective in achieving both high productivity and high molecular weight of P(3HB). 相似文献
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M. D. Srinivas 《Pramana》2003,60(6):1137-1152
We derive an optimal bound on the sum of entropic uncertainties of two or more observables when they are sequentially measured
on the same ensemble of systems. This optimal bound is shown to be greater than or equal to the bounds derived in the literature
on the sum of entropie uncertainties of two observables which are measured on distinct but identically prepared ensembles
of systems. In the case of a two-dimensional Hilbert space, the optimum bound for successive measurements of two-spin components,
is seen to be strictly greater than the optimal bound for the case when they are measured on distinct ensembles, except when
the spin components are mutually parallel or perpendicular 相似文献
124.
Svitlana P. Rogovchenko 《Journal of Mathematical Analysis and Applications》2003,279(1):121-134
In this paper, we are concerned with a class of nonlinear second-order differential equations with a nonlinear damping term. Passage to more general class of equations allows us to remove a restrictive condition usually imposed on the nonlinearity, and, as a consequence, our results apply to wider classes of nonlinear differential equations. Two illustrative examples are considered. 相似文献
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线性规划联合算法的理论与应用 总被引:6,自引:4,他引:2
本在[1]的基础上.较系统的叙述了线性规划联合算法的步骤、相关理论及其应用,指出该算法具有避免人工变量、减少迭代次数、使用灵活、应用方便等特点。 相似文献
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