Revealing the Cell Entry Dynamic Mechanism of Single Rabies Virus Particle

The rabies virus is a neurotropic virus that causes fatal diseases in humans and animals. Although studying the interactions between a single rabies virus and the cell membrane is necessary for understanding the pathogenesis, the internalization dynamic mechanism of single rabies virus in living cells remains largely elusive. Here, we utilized a novel force tracing technique based on atomic force microscopy(AFM) to record the process of single viral entry into host cell. We revealed that the force of the rabies virus internalization distributed at (65±25) pN, and the time was identified by two peaks with spacings of (237.2±59.1) and (790.3±134.4) ms with the corresponding speed of 0.12 and 0.04 μm/s, respectively. Our results provide insight into the effects of viral shape during the endocytosis process. This report will be meaningful for understanding the dynamic mechanism of rabies virus early infection.     

Synthesis,Crystal Structure and Photoluminescence of a Three-coordinate Ag(Ⅰ) Complex

A three-coordinate Ag(I) complex, [Ag(Mepzpy)(PAr3)]BF4·H2O(1, Mepzpy = 3-methyl-1-(2-pyridyl)pyrazole, PAr3 = tri(o-tolyl)phosphine), was synthesized from the reaction of Ag(CH3CN)4BF4, PAr3 and Mepzpy in CH3 CN at room temperature. The compound was characterized by UV-vis, NMR and X-ray single-crystal structure analysis. It crystallizes in triclinic space group P1 with a = 10.2251(5), b = 10.6014(5), c = 15.7012(5) , α = 92.963(3), β = 92.641(3), γ = 114.647(4)o, V = 1540.6(1) 3, Z = 2, Mr = 676.24, Dc = 1.458 g/cm3, F(000) = 688, μ = 6.187 mm-1, GOOF = 1.038, the final R = 0.0530 and w R = 0.1493 for 6371 observed reflections with I 2σ(I). Compound 1 is ionic. It is composed of a BF4- anion and a [Ag(Mepzpy)(PAr3)]+ cation. The Ag(I) ion adopts a distorted trigonal pyramidal coordination geometry defined by two nitrogen atoms and a phosphorous atom. The complex emits blue luminescence with maximum peaks at 470 nm in solid state at room temperature.     

高效液相色谱-电喷雾串联质谱法测定畜禽粪便中四环素类抗生素

建立了高效液相色谱-电喷雾串联质谱法(HPLC-MS/MS)测定畜禽粪便中四环素类抗生素的分析方法。样品经甲醇-乙酸-水(体积比6∶3∶1)溶液提取,OASIS HLB小柱净化后,采用Waters Symmetry C18色谱柱(150×3.9mm,5μm)进行分离,以0.3%甲酸水溶液-0.3%甲酸乙腈溶液梯度洗脱,电喷雾正离子模式多反应监测(MRM)模式定量分析。在优化的色谱条件下,四环素、土霉素和金霉素在0.05~1.0mg/L范围内具有良好的线性,其检出限分别为0.025、0.084、0.098mg/kg;样品回收率为80%~103%。实验结果表明,该方法较液相色谱法具有更高的准确性和灵敏度,可满足畜禽粪便中四环素类抗生素残留的检测要求。     

航空发动机旋转叶片振动监测系统研究

介绍了叶尖定时测量旋转叶片的原理，设计的光纤柬式传感结合光电接收实现了高精度的弱光检测。基于“5＋2”传感器均布方案，利用双速率对叶片振动信号进行采样，通过“5＋2”处理算法实现了振动频率的准确辨识，并在某航空设备上进行了高速模拟转子实验，实验表明测得频率与应变仪结果一致。     

磁控溅射制备ZnO薄膜的受激发射特性的研究

用射频磁控反应溅射法在二氧化硅衬底上制备ZnO薄膜。得到了在不同温度下ZnO薄膜的吸收与光致发光。观测到了纵光学波 (LO)声子吸收峰与自由激子吸收峰 ;室温 (30 0K)下 ,PL谱中仅有自由激子发光峰。这些结果证实了ZnO薄膜具有较高的质量。探讨了变温ZnO薄膜的发光特性。研究了ZnO薄膜的受激发射特性。     

双硫腙-活性炭柱富集分离水中痕量铅的条件研究

本文研究了用活性炭加双硫腙柱富集、分离水中痕量铅的适宜条件。用火焰原子吸收法分析了不同分离条件下铅的回收率。并讨论了几种干扰离子的影响情况。     

并联铁电体冲击去极化输出电流实验和计算

 为分析不同数量铁电体的输出电流特征，进行了多片并联PZT95/5铁电体冲击去极化实验，测量了电阻负载下铁电体的输出电流；建立了并联铁电体输出电流的计算模型，计算了并联铁电体的输出电流。通过比较计算和实验结果，分析了铁电体数量和冲击波状态对铁电体输出电流的影响。结果表明：若进入铁电体的冲击波为平面波，铁电体输出电流的波形接近方波，电流峰值随铁电体并联数量的增加成比例增加，冲击波波形和侧向稀疏波对铁电体输出电流波形和去极化率有很大的影响。     

喷雾热解法制备的SrWO4膜的阴极射线发光

利用超声喷雾热解法制备了钨酸锶SrWO₄多晶发光膜,并研究了制备条件及掺杂对其阴极射线发光特性的影响.生成的发光膜在300℃以上退火后具有白钨矿结构,其阴极射线发光为一宽带的蓝光,包括一个位于448nm的蓝色发光带和一个位于488.6nm的蓝绿色发光带,是由阴离子络合物WO₄^2-的电荷转移跃迁引起的.发光强度随着退火温度的升高而增强,而退火气氛对其影响不大.在SrWO₄膜中掺入银离子Ag⁺和镧离子La³⁺后,不影响其发光特性,但铕离子Eu³⁺的掺入对发光特性有影响.     

多晶X射线线形傅氏分析方法在合金材料力学性能预测上的应用

缺陷组态直接涉及到结构材料的力学性能,特别是位错亚结构(DSS)为最重要的缺陷组态中的第一个。本文评论并叙述了一种由王煜明完善并发展了的关于DSS的实用X射线线形傅里叶综合分析方法。根据此法进行了定量预测合金材料的力学性能,同时证明本方法的实际应用价值。 关键词：