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基于导电聚合物吡咯膜的生物电化学传感研究 总被引:1,自引:0,他引:1
导电聚合物可以与纳米尺寸的生物分子形成纳米复合物,在生物催化传感器的设计中,聚吡咯(Ppy)是应用最为广泛的一种导电聚合物,这种聚合物膜具有良好的导电性、选择性、稳定性、灵敏性以及生物相容性,很容易用于纳米尺寸生物分子的固定,并展示独特的催化和亲合特性.文章主要讨论了氧化还原酶在Ppy界面上的电子传递,提出了Ppy在免疫传感器、DNA传感器以及分子印迹技术领域中的最新应用及存在的问题,并对导电聚合物在未来分子技术中的发展进行了展望. 相似文献
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以邻苯二胺(o-PD)为功能单体, 乙二胺四乙酸铜离子螯合物(Cu(II)-EDTA)为模板分子, 利用循环伏安法(CV)合成了Cu(II)-EDTA分子印迹聚合物(MIPs). 通过紫外-可见(UV-Vis)光谱、X射线光电子能谱(XPS)、差分脉冲伏安法(DPV)、石英晶体微天平(QCM)等手段对合成的聚合物进行了表征. UV-Vis光谱分析表明当溶液的pH≥5.0时有利于邻苯二胺电聚合形成聚合度较高的聚合物; XPS结果证明Cu(II)-EDTA螯合物被成功地包覆在聚合物膜中, 且推断出模板分子和聚合物之间可能主要靠氢键相互作用; DPV实验结果证明模板分子能够被有效洗脱; QCM的测试结果表明此方法合成的Cu(II)-EDTA印迹聚合物膜对Cu(II)-EDTA具有良好的响应度. 相似文献
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合成了可对氨基酸海因对映异构体选择性分离的分子印迹聚合物膜。利用紫外光谱法比较不同功能单体与模板分子的作用能力。以丙烯酰胺为功能单体,在极性溶剂中制备了5R-5氨-基酸海因的分子印迹聚合物膜,通过Scatchard分析法研究膜中结合位点情况。通过膜透过实验研究印迹膜对外消旋体的分离特性。Scatchard分析显示聚合物膜中形成了两类结合位点,其解离常数分别为1.88mmol/L和5.14mmol/L;选择性透过实验表明膜中形成了与5R-5氨-基酸海因分子形状和功能基因位置匹配的孔穴。与非印迹聚合物膜相比,印迹聚合物膜对对映体具有良好的选择性。 相似文献
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以溶菌酶为模板蛋白质,结合分子印迹技术在硅烷化的基质玻片上制备了溶菌酶分子印迹聚合物膜。实验优化了溶菌酶聚合物膜的印迹体系,考察了溶菌酶分子印迹聚合物膜的吸附平衡时间、最大吸附量、特异识别能力、重复使用性以及对实际样品中溶菌酶的分离情况。结果表明,在最优条件下,制备的分子印迹聚合物膜对溶菌酶具有特异吸附能力,印迹因子为3.0,吸附平衡时间为5 min,吸附行为符合Langmuir吸附模型,理论最大吸附量为42.5 mg/g,实际样品中的吸附量为30 mg/g。且此印迹聚合物膜在重复使用5次后,最大吸附量仅下降了5%,具有良好的重复使用性。该方法为复杂生物样品中目标蛋白质的分离富集提供了一种快速、高效的手段。 相似文献
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琥珀酸氯霉素分子印迹聚合膜的制备及其吸附特性研究 总被引:2,自引:0,他引:2
为制备对琥珀酸氯霉素分子具有特异性吸附的分子印迹聚合物膜, 利用模板分子琥珀酸氯霉素(HS-CAP)、功能单体甲基丙烯酸(MA)、交联剂乙二醇二甲基丙烯酸酯(EGDMA)、引发剂偶氮二异丁腈(AIBN)、溶剂四氢呋喃, 采用紫外光引发聚合的方法制备出含有HS-CAP分子印迹位点的印迹聚合微粒, 并使用相转化的方法, 制备含有这种HS-CAP分子印迹微粒的醋酸纤维素膜. 然后通过吸附实验检测该印迹膜的吸附特性, 与非印迹膜相比, 印迹膜对模板分子具有良好的特异性识别作用, 与印迹膜相互作用的模板分子溶液, 在作用前后浓度发生了显著的变化; 印迹膜对模板分子的识别作用主要集中于与模板分子相互作用的最初2 h之内, 并随作用时间的延长而降低; 当模板分子浓度介于0.2~0.0125 mg/mL这一范围内时, 模板分子溶液浓度越高, 印迹膜的吸附特性越明显. 本实验所制备的分子印迹聚合膜对模板分子具有特异性识别能力, 可以在下一步研制以分子印迹聚合膜为基础的检测氯霉素残留的传感设备中得到应用. 相似文献
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β-环糊精(β-CD)及其衍生物作为一种新兴的功能单体在分子印迹技术中得到了越来越多的应用。β-CD及其衍生物能够与许多分子形成主-客体包合物,基于这一包合作用制备的分子印迹聚合物具有稳定性好和选择性高等优点,因此在具有复杂基质的环境和食品样品中目标化合物的选择性分离和富集中得到了重视和发展。该综述主要回顾了2013年以来文献中报道的一些基于β-CD及其衍生物作为功能单体的分子印迹聚合物在环境水和食品样品前处理方面的最新应用,揭示这一分子印迹聚合物在复杂样品前处理中的优势。 相似文献
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In this research, a surface imprinting strategy has been adopted in protein imprinting. Bovine hemoglobin surface-imprinted
polystyrene (PS) nanoparticles with magnetic susceptibility have been synthesized through multistage core-shell polymerization
system using 3-aminophenylboronic acid (APBA) as functional and cross-linking monomers. Superparamagnetic molecularly imprinted
polystyrene nanospheres with poly(APBA) thin films have been synthesized and used for the first time for protein molecular
imprinting in an aqueous solution. The magnetic susceptibility is imparted through the successful encapsulation of Fe3O4 nanoparticles. The morphology, adsorption, and recognition properties of superparamagnetic molecularly imprinted polymers
(MIPs) have been investigated using transmission electron microscopy, X-ray diffraction, thermogravimetric analysis, and vibrating
sample magnetometer. Rebinding experimental results show that poly(APBA) MIPs-coated superparamagnetic PS nanoparticles have
high adsorption capacity for template protein bovine hemoglobin and comparatively low nonspecific adsorption. The imprinted
superparamagnetic nanoparticles could easily reach the adsorption equilibrium and achieve magnetic separation in an external
magnetic field, thus avoiding some problems of the bulk polymer. 相似文献
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Melamine was chosen as template, methacrylic acid was chosen as functional monomer, and divinylbenzene, ethylene glycol dimethacrylate, trimethylolpropane trimethylacrylate were chosen as cross‐linking agents, respectively. The WB97XD/6‐31G(d, p) method was used to calculate the geometry optimization of the different imprinting ratios, the action sites, the bonding situation, and the optimization of the cross‐linking agents. The nature of the imprinting effect was also studied by the atoms in molecules theory. The theoretical results showed that melamine interacts with methacrylic acid by hydrogen bonding, and the melamine molecularly imprinted polymers with a molar ratio of 1:6 have the most hydrogen bonds and the most stable structure. Divinylbenzene is the best cross‐linking agent for the melamine molecularly imprinted polymers. The melamine molecularly imprinted polymers were synthesized by precipitation polymerization. The results showed that the maximum adsorption capacity for molecularly imprinted polymers towards melamine is 19.84 mg/g, and the adsorption quantity of the polymers to melamine is obviously higher than that of cyromazine, cyanuric acid, and trithiocyanuric in milk. This study could provide theoretical and experimental references for the screening of the imprinting ratio and the cross‐linking agent for the given template and monomer system. 相似文献
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Sharma PS Pietrzyk-Le A D'Souza F Kutner W 《Analytical and bioanalytical chemistry》2012,402(10):3177-3204
This critical review describes a class of polymers prepared by electrochemical polymerization that employs the concept of
molecular imprinting for chemical sensing. The principal focus is on both conducting and nonconducting polymers prepared by
electropolymerization of electroactive functional monomers, such as pristine and derivatized pyrrole, aminophenylboronic acid,
thiophene, porphyrin, aniline, phenylenediamine, phenol, and thiophenol. A critical evaluation of the literature on electrosynthesized
molecularly imprinted polymers (MIPs) applied as recognition elements of chemical sensors is presented. The aim of this review
is to highlight recent achievements in analytical applications of these MIPs, including present strategies of determination
of different analytes as well as identification and solutions for problems encountered. 相似文献
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Molecularly imprinted polymer sensors for pesticide and insecticide detection in water. 总被引:10,自引:0,他引:10
Antibodies, peptides, and enzymes are often used as molecular recognition elements in chemical and biological sensors. However, their lack of stability and signal transduction mechanisms limits their use as sensing devices. Recent advances in the field of molecularly imprinted polymers (MIPs) have created synthetic materials that can mimic the function of biological receptors but with less stability constraints. These polymers can provide high sensitivity and selectivity while maintaining excellent thermal and mechanical stability. To further enhance the advantages of the traditional imprinted polymer approach, an additional fluorescent component has been introduced into these polymers. Such a component provides enhanced chemical affinity as well as a method for signal transduction. In this type of imprinted polymer, binding of the target analyte invokes a specific spectral signature from the reporter molecule. Previous work has provided molecularly imprinted polymers that are selective for the hydrolysis products of organophosphorus species such as the nerve agents sarin and soman. (A. L. Jenkins, O. M. Uy and G. M. Murray, Anal. Chem., 1999, 71, 373). In this paper the direct imprinting of non-hydrolyzed organophosphates including pesticides and insecticides is described. Detection limits for these newly developed MIP sensors are less than 10 parts per trillion (ppt) with long linear dynamic ranges (ppt to ppm) and response times of less than 15 min. 相似文献
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Xiaoling Qi Xiaoming Yang Bin Wang Hong Yang 《International journal of environmental analytical chemistry》2013,93(3):268-278
A broad selective molecularly imprinted polymers-based solid phase extraction (MISPE) for levonorgestrel (LNG) from water samples was developed. Using LNG as a template molecule, acrylamide (AA) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as linking agent and bulk polymerisation as a synthetic method, the molecularly imprinted polymers (MIPs) were synthesised and characterised. The MIPs displayed a high specific rebinding for LNG with the imprinting factor of 3.71. The Scatchard analysis showed that there was at least one class of binding site for LNG formed in the MIPs with the dissociation constant of 8.046?µg?mL?1. The results of selectivity testing indicated that the MIPs also exhibited high cross-reactivity with structurally related compounds (estrone, methylprednisolone and ethinyl estradiol), but no recognition with non-structurally related compound (indomethacin), suggesting that the MIPs could be used as a broad recognition absorbent. MISPE column was prepared by packing MIPs particles into a common SPE cartridge. The MISPE extraction conditions including loading, washing and eluting solutions were carefully optimised. Water samples spiked with LNG were extracted by MISPE column and detected by high-performance liquid chromatography. The recoveries were found to be 79.97?~?132.79% with relative standard deviations (RSD) of 1.92?~?10.43%, indicating the feasibility of the prepared MIPs for LNG extraction. 相似文献
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表面分子印迹材料和技术在分离分析中的应用进展 总被引:1,自引:0,他引:1
复杂体系的高选择性分析对分离新材料和新方法提出了迫切需求。分子印迹聚合物(MIPs)以其特异性高、化学稳定性好、制备简单且成本低等优点,在高选择性分离分析中展现出巨大的应用前景。但以本体聚合为代表的传统合成方法获得的MIPs存在识别位点位于聚合物内部难以识别、模板分子洗脱不彻底、传质速率慢、结合容量低等问题。表面印迹技术制备的核-壳型表面分子印迹材料是解决上述难题的有效途径。通过核体和壳层结构的设计和构建,表面分子印迹材料还可具备多功能、多响应的特性,适于现代分离分析对快速、高效、高选择性的要求。该文主要综述了近几年表面分子印迹技术在样品前处理、化学/生物传感分析及靶向药物递送领域的应用进展。 相似文献
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Yan Wang Jun-bo Liu Shan-shan Tang Zheng-qiang Dai Rui-fa Jin 《Structural chemistry》2016,27(3):897-905
The long-range correction method (WB97XD) was applied to simulate the self-assembly system of the molecularly imprinted polymers via Gaussian 09 software. Melamine (MAM) was taken as the template molecule and trifluoromethacrylic acid (TFMAA) was taken as the functional monomer. The ethylene glycol dimethacrylate, divinylbenzene, pentaerythritol triacrylate, and trimethylolpropane trimethylacrylate were chosen as the cross-linking agents, respectively. The acetonitrile, methanol, dichloromethane, chloroform, toluene, ethanol, and dimethylsulfoxide were taken as solvents, respectively. The bonding situation, the geometry optimization of the different imprinting ratios, the binding energy, the molecular imprinting mechanism between MAM and TFMAA, and the influence of cross-linking agent as well as solvent have been studied. The detailed topological property was also applied to discuss the nature of the imprinting effect. The results indicate that MAM and TFMAA can form ordered compounds via hydrogen bond interaction. The melamine-molecularly imprinted polymers with a molar ratio of 1:6 have the lowest binding energy, the largest amount of hydrogen bonds, and the stable structure in toluene solvent. Divinylbenzene is the best cross-linking agent for the melamine-molecularly imprinted polymers in comparison with others. The study can provide a theoretical reference for the synthesis of the high selectivity melamine-molecularly imprinted polymers. 相似文献
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Giuseppe Cirillo Francesco Puoci Manuela Curcio Ortensia Ilaria Parisi Francesca Iemma Umile Gianfranco Spizzirri Nevio Picci 《Colloid and polymer science》2010,288(6):689-693
The aim of this study is the preparation of molecularly imprinted polymers by employing a redox pair as initiator system.
Bulk molecularly imprinted polymers were synthesized by using Fenton reagents as initiator system. Theophylline, methacrylic
acid, and ethylene glycol dimethacrylate were employed as model template, functional monomer, and crosslinking agent, respectively.
Conventional imprinted polymers were also prepared by using 2,2′-azoisobutyronitrile in order to evaluate the efficiency of
the proposed initiator system. Redox molecularly imprinted polymers and conventional molecularly imprinted polymers were characterized
by water uptake measurement, while the imprinting effect of synthesized polymers were evaluated by performing binding experiments
in organic (acetonitrile) and in water (buffered water solution at pH = 7.4) media. 相似文献
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In this study, poly(amino acid)-based thermoresponsive molecularly imprinted magnetic nanoparticles for recognition and release of lysozyme was prepared via surface imprinting method. For constructing the molecularly imprinted polymer (MIP) layer, amino acid-based thermoresponsive monomer (N-methacryloyl-l-alanine methyl ester, MA-L-Ala-OMe) was mainly selected for the functional monomer along with N,N′-methylenebis(acrylamide) as the crosslinker. The resultant magnetic MIP nanoparticles were characterized in detail. Meanwhile, the dynamic light scattering studies and swelling ratios measurements were carried out for demonstrating the thermoresponsive property of the imprinted nanoparticles. The prepared magnetic MIP nanoparticles showed good adsorption capacity and selective recognition properties to lysozyme. Moreover, the fast adsorption process could reach equilibrium within 15 min. Importantly, the capture and release of lysozyme could be easily realized simply by altering the temperature of aqueous solution. Furthermore, the prepared imprinted nanoparticles were applied to separate lysozyme from the real egg white samples. The results proved that the thermoresponsive MIPs based on MA-L-Ala-OMe have great potential for selectively enriching target proteins in real samples. 相似文献