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A γ-hydroxyphosphonate P6 (O1-methyl-O2-(1, 2, 2-trimethylpropyl)-2-hydroxy-5-nitro-phenyl methylphosphonic acid) which is proposed to be an analog of the transition state in hydrolysis of soman was synthesized. Artificial antigens were obtained by conjugating P6 to the carrier proteins BSA (bovine serum albumin) and LPH (Limulus polyphenus hemocyanin). Mice were immunized with P6-LPH and recombinant single-chain antibody phage display library was constructed. After 4 rounds of panning against P6-BSA and competitive inhibition enzyme immunoassay, more than 70 strains of phage antibodies capable of binding soman were obtained and 11 of them can accelerate the hydrolysis reaction of soman. One of them (EP6) was studied further. Soluble single-chain antibody was prepared and purification was performed by gel filtration and ion exchange chromatography. The kinetic experiment was carried out showing that the turnover number kcatt= 198 min-1 and the rate enhancement kcatkuncat = 122 419. When 0.16 mg · mL-1 相似文献
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通过相应的酸和亚硫酰氯反应合成了3种新的稠环芳基取代酰氯类化合物(9-蒽丙烯酰氯,9-蒽丙酰氯和1-芘丙烯酰氯)作为羟基药物及毒物超微量分析的荧光探针。 相似文献
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含哌啶结构杂环醇的立体化学研究 总被引:2,自引:0,他引:2
用分子力学和量子化学计算方法(AM1)研究了含哌啶结构杂环醇(1_4)及其羧酸酯类化合物的立体化学问题.结果表明,在哌啶醇(1)及醇(3)分子中,4位羟基处于哌啶环的a键位时,环上的氮原子和羟基氧原子之间可能形成非经典的轨道超共轭作用,使其构型构象得到稳定. 相似文献
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通过邻苯二胺与环戊酮的β-羧酸酯缩合及催化氢化,合成了由环戊基与苯并二氮并环形成的新的苯并二氮类三环化合物。 相似文献