增效试剂对过氧化物模拟酶催化显色体系的增效作用——Fe-TPPS_4-4-AAP-苯酚衍生物-H_2O_2体系

本文以β－环糊精（β－ＣＤ）和表面活性剂为增效试剂，分别研究了它们对以Ｆｅ－ｍｅｓｏ－（四（４－磺基苯）卟啉）（Ｆｅ－ＴＰＰＳ４）为催化剂，催化过氧化氢氧化４－氨基安替比林（４－ＡＡＰ）与苯酚衍生物显色反应的速度和灵敏度的影响，发现β－ＣＤ和十二烷基苯磺酸钠（ＳＤＢＳ）对该体系具有明显的增效作用．在３×１０－３ｍｏｌ／Ｌβ－ＣＤ存在下，测定Ｈ２Ｏ２的灵敏度比文献报道的提高了１．５６倍．利用ＳＤＢＳ浓度与催化显色反应初速度的增加值之间的线性关系，建立了测定微量ＳＤＢＳ的方法     

毕兹多克隆抗体的制备和分析应用

用琥珀酸酐－碳二亚胺法和氯磺化法合成了毕兹（Ｂｚ）的两种人工抗原Ｂｚ＿ＨＳ＿ｐｒｏｔｅｉｎ和Ｂｚ＿ＳＯ２＿ｐｒｏｔｅｉｎ。由Ｂｚ＿ＳＯ２＿ＢＳＡ（牛血清白蛋白）诱导出的抗血清能够用来测定１０－９ｍｏｌ·Ｌ－１的Ｂｚ，工作曲线的线性范围在３×１０－５～３×１０－９ｍｏｌ·Ｌ－１，相对标准偏差为１３６％（ｎ＝６），回收率在９０％～１１５％。     

1,2,5-噻二唑衍生物电子结构的紫外光电子能谱研究

首次报导了１,２,５－噻二唑衍生物３－氯－１,２,５－噻二唑（Ａ）和３,４＝二氯－１,２,５－噻二唑（Ｂ）化合物的紫外光电子能谱（ＵＰＳ）,谱带的指认建筑在对谱带形状、相对强度、实验电离能（ＩＰＳ）的分析以对研究分子Ｇａｕｓｓｉａｎ ９４ＳＴ０－６Ｇ从头计算电离能（－ε１）。化合物Ｂ ＵＰＳ谱带的ＩＰＳ比相应的化合物Ａ的ＩＰＳ均你芝归结为Ｂ分子中两个取代Ｃ１原子上电子的拥挤效应。计算的Ｂ的 有量（     

以混合模板剂合成TS—1分子筛及其性能研究

以四丁基溴化铵（ＴＢＡＢｒ）＋四乙基氢氧化铵（ＴＥＡＯＨ，ｎ（ＴＢＡ＾＋）／ｎ（ＴＥＡ＾＋）＝１）或以四丙基溴化铵（ＴＰＡＢｒ）＋ＴＥＡＯＨ为模板剂，钛酸四丁酯和正硅酸乙酯为原料，于１７０℃水热合成出ＴＳ－１分子筛。对合成的ＴＳ－１样品进行了ＸＲＤ，ＦＴ－ＩＲ，ＳＥＭ和ＢＥＴ比表面积分析，证实了样品中钛已进入Ｓｉｌｉｃａｌｉｔｅ－１骨架。选择戊烷氧化为探针反应，考察了ＴＳ－１的催化活性。结果表明以     

Synthesis and X－ray Structure of a Binuclear Nickel（Ⅱ） Complex of Nickel（Ⅱ） Perchlorate and 2，2＇－Bipyridyl with N，N＇－Bis（2－aminoethyl）oxamido Nickel（Ⅱ）

ＳｙｎｔｈｅｓｉｓａｎｄＸ－ｒａｙＳｔｒｕｃｔｕｒｅｏｆａＢｉｎｕｃｌｅａｒＮｉｃｋｅｌ（Ⅱ）ＣｏｍｐｌｅｘｏｆＮｉｃｋｅｌ（Ⅱ）Ｐｅｒｃｈｌｏｒａｔｅａｎｄ２,２＇－ＢｉｐｙｒｉｄｙｌｗｉｔｈＮ,Ｎ＇－Ｂｉｓ（２－ａｍｉｎｏｅｔｈｙｌ）ｏｘａｍｉｄ...     

新型六核钼原子簇化合物{[Mo3（μ3—S）（μ—S2）2（dtp）3]—（μ3—S）（η…

［Ｍｏ３（μ３－Ｏ）（μ－Ｓ）３（μ－ＯＡｃ（ｄｔｐ）３（ｐｙ）］和［Ｍｏ３（μ－Ｓ）３（μ－ＯＡｃ（ｄｔｐ）３－（ｐｙ）］（ｄｔｐ＝Ｓ２Ｐ（ＯＣ２Ｈ５）２＾－）族合物分别与金属化合物ＢｉＩ３和ＳｂＢｒ３进行原子簇反应均能获得意外产物｛［Ｍｏ３（μ３－Ｓ）（μ－Ｓ２）２（ｄｔｐ）３］（μ３－Ｓ）（η＾３－Ｓ２）［Ｍｏ２（μ３－Ｓ）（μ－Ｓ）３（μ－ＯＡｃ）（ｄｔｐ）２］｝六核钼簇。其结构实际上是由     

DTPA－Eu~（3＋）螯合物在核酸杂交分析中的应用

报道了靶ＤＮＡ／生物素化λＤＮＡ探针／亲合素／Ｂｉｏ－ＢＳＡ（ＴＧ）－ＤＴＰＡ－Ｅｕ~（３＋）体系在核酸杂交分析中的应用。亲合素做为连结杂交体与Ｂｉｏ－ＢＳＡ－ＤＴＰＡ－Ｅｕ~（３＋）或Ｂｉｏ－ＴＧ－ＤＴＰＡ－Ｅｕ~（３＋）的桥，生物素和ＤＴＰＡ－Ｅｕ~（３＋）均标记在载体蛋白（ＢＳＡ，ＴＧ）上。优化了分析条件，比较了己二胺、乙二胺转胺化探针及载体蛋白对杂交分析的影响。方法灵敏度高（１ｐｇ／ｗｅｌｌ靶λＤＮＡ），重现性较好（ＲＳＤ＝５．７％，ｎ＝６）。     

1－苯基－3－甲基－4－（a－呋喃甲酰基）－5－吡唑酮与中性萃取剂协同萃取镱的研究

研究了１－苯基３－甲基－４－（呋喃甲酰基）－５－吡唑酮（ＨＡ）萃取镱及其分别与丁基辛基亚砜（ＢＯＳＯ）、戊基已基亚砜（ＰＨＳＯ）、乙基十二烷基亚砜（ＥＤＳＯ）、二苯亚砜（ＤＰＳＯ）和ＴＢＰ协同萃取镱的行为。协萃配合物的组成为ＹｂＡ＿３·Ｂ，ＨＡ的萃取平衡常数为—５．６４±０．０４，中性萃取剂的协萃能力比单独萃取时高３～４个数量级，协萃效果明显。     

增效试剂对过氧化物模拟酶催化显色体系的增效作用——Fe—TPPS4—4—AAP—苯酚

本以β－环糊精（β－ＣＤ）和表面活性剂为增效剂，分别研究了它们对以Ｆｅ－ｍｅｓｏ－（四（４－磺基苯）卟啉）（Ｆｅ－ＴＰＰＳ４）为催化剂，催化过氧化氢氧化４－氨基安替比林（４－ＡＡＰ）与苯酚衍生物显色反应的速度和灵敏度的影响，发现β－ＣＤ和十二烷基苯磺酸钠（ＳＤＢＳ）对该体系具有明显的增效作用，在３×１０＾－３ｍｏｌ／Ｌβ－ＣＤ存在下，测定Ｈ２Ｏ的灵敏度比献报道的提高了１．５６倍，利用ＳＤＢＳ深     

SyntesesandMagnetismofBinuclearCu（Ⅱ）－Ni（Ⅱ）ComplexeswithN，Nbis（N－hydroxyisopropyl－ethyleneamine）－oxamidoasBridgingLigand

ＳｙｎｔｅｓｅｓａｎｄＭａｇｎｅｔｉｓｍｏｆＢｉｎｕｃｌｅａｒＣｕ（Ⅱ）－Ｎｉ（Ⅱ）ＣｏｍｐｌｅｘｅｓｗｉｔｈＮ,Ｎｂｉｓ（Ｎ－ｈｙｄｒｏｘｙｉｓｏｐｒｏｐｙｌ－ｅｔｈｙｌｅｎｅａｍｉｎｅ）－ｏｘａｍｉｄｏａｓＢｒｉｄｇｉｎｇＬｉｇａｎｄ￣＊ＳＨＩＪ...     

孔石莼质体蓝素的柱色谱纯化及其N-端氨基酸序列的分析测定

通过DEAE-Sepharose Fast Flow阴离子交换柱和Sephadex G-75凝胶过滤柱分离纯化得到了孔石莼(Ulva pertusa)的质体蓝素。其步骤为:将孔石莼样品以0.02 mol/L磷酸盐缓冲液(pH 7.2)进行匀浆,然后离心去除沉淀,将上清液用硫酸铵分级盐析获得饱和度为40%~80%的盐析蛋白;通过DEAE-Sepharose 柱色谱,在含有0~1.0 mol/L NaCl 的0.01 mol/L磷酸盐缓冲液线性梯度洗脱下,盐析蛋白有3个主要的洗脱峰,然后在Sephadex G-75凝胶过滤色谱柱中进一步纯化。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）显示,该蛋白质被纯化为单一条带。根据蛋白质电泳迁移率,纯化蛋白质的相对分子质量约为10000。该蛋白质不含糖。纯化的蛋白质经电转移至聚偏二氟乙烯（PVDF）膜后,以Edman降解法进行N-端氨基酸序列测定,前20个氨基酸残基序列为AAIVKLGPDDGSLAFVPSKI。通过对相关蛋白质数据库的检索,发现该序列与3种已报道的海藻的质体蓝素具有较高的序列同源性,其同源性分别为85%,85%和90%。据此,认为孔石莼的质体蓝素已获得纯化,其N-端20个氨基酸残基与已报道的海藻质体蓝素的氨基酸残基有较大的同源性,也存在着一定的变异。     

Purification of antibody heteropolymers using hydrophobic interaction chromatography

A heteropolymer (HP) is a unique dual antibody conjugate composed of specific, chemically cross-linked monoclonal antibodies (mAbs). In this study we have demonstrated that HPs can be purified using hydrophobic interaction chromatography (HIC). Two propyl HIC resins; [PolyPropyl A and EMD Fractogel Propyl (S)] were evaluated in this study. Phosphate buffers, pH 6.5 containing ammonium sulfate or sodium sulfate were used to bind the HP to the column. A descending sulfate gradient or step gradient was used to elute the bound HP species from the column. The HP reaction mixture typically contains multiple conjugated HP species, as well as unreacted monomer mAbs. Conjugated HP product was successfully separated from unreacted antibody monomers with both propyl resins using buffers with ammonium sulfate. There was no monomer separation from HP using buffers with sodium sulfate. The purification processes, presented in this study allows the non-cross-linked antibodies to pass through the column without being bound to the resin, while the cross-linked antibodies (the HP product) bound to the column were subsequently eluted by decreasing the ammonium sulfate concentration in the running buffer. HP product was efficiently separated from free mAbs using Propyl HIC resins at both analytical and preparative scales.     

Orthogonal test design for optimization of suitable conditions to separate C-phycocyanin from Spirulina platensis by high-speed counter-current chromatography using reverse micelle solvent system

High-speed counter-current chromatography (HSCCC) was applied to separate C-phycocyanin (C-PC) from Spirulina platensis in the article. The suitable conditions were optimized by an orthogonal test design (L(9)(3)(3)), including the stationary phase of reverse micelle solvent system (0.10 g/mL cetyltrimethylammonium bromide [CTAB]/isooctane-hexylalcohol), mobile phase A (0.05 mol/L sodium phosphate buffer, pH 4.0, containing 0.2 mol/L KCl) and mobile phase B (0.05 mol/L sodium phosphate buffer, pH 8.0, containing 0.4 mol/L KCl). Under the selected conditions, 78.7 mg protein was purified from 200 mg crude extract of S. platensis, and the purity of the product was 4.25 based on the absorbance ratio of A(620)/A(280) , which was increased 6.85 times compared with the crude extract. Then, the protein was identified to be C-PC by MALDI-TOF/TOF-MS and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis compared with the standard. The application of HSCCC used in the separation of C-PC from S. platensis was first reported in the article. Furthermore, three kinds of tumor cell lines including human hepatoma cell line SMMC-7721, human ovarian carcinoma cell line ES-2, and human lung adenocarcinoma cell line SPCA-1 were used to evaluate the anticancer activities of the separated product, and the results showed that the separated C-PC had excellent anti-tumor actions with the IC(50) values at 2.998, 4.854, and 8.423 μg/mL, respectively, for 48 h treatment. The outcome indicates that an effective method for C-PC purification by HSCCC has been established.     

免疫法制备液相色谱生物样品的研究——血中沙丁胺醇的分析

 用动物免疫法制备了免疫亲和柱纯化水溶性的沙丁胺醇血浆样品。琥珀酸酐交联沙丁胺醇和牛血清白蛋白获得抗原免疫家兔抗沙丁胺醇抗体——免疫球蛋白。琼脂糖Ｓｅｐｈａｒｏｓｅ４Ｂ与抗体交联制成免疫球蛋白亲和柱。对高效液相色谱法测定中的一般提取方法和固相小柱提取法作了比较，后者具有内源性杂质干扰少的优点，是生物样品预处理的一种有效的方法。     

一种从蛇毒中纯化神经生长因子的新工艺

为了能从中华眼镜蛇蛇毒中简单快速地分离纯化神经生长因子（一种治疗各种神经性损伤和神经退行疾病的药物,简称NGF）,采用不同的色谱柱联用的方式对NGF的纯化工艺进行了研究。结果表明,采用DEAE Sepharose F.F.和Sephadex G 50二步柱色谱工艺可以从蛇毒中快速分离得到神经生长因子。经十二烷基硫酸钠聚丙烯酰胺凝胶电泳和反相高效液相色谱分析, 证明所得到的NGF为单一组分,相对分子质量约为 29 000。对8　d龄鸡胚背根神经节采用体外培养法,结果证明,所得NGF具有明显的促进神经纤维     

A SIMPLE AND IMPROVED METHOD OF ISOLATION AND PURIFICATION FOR NATIVE OAT PHYTOCHROME

Abstract— A simple procedure for the isolation and purification of 124 kDa phytochrome (phyA) from etiolated Avena seedlings has been developed employing ammonium sulfate back-extraction. After solubilization of the ammonium sulfate precipitate (250 g/L) an additional ammonium sulfate fractionation with 17 g per 100 mL rather than column chromatography was performed. After several steps of the "washing-out" procedure with 100 mM phosphate buffer, phytochrome was solubilized in 10 m M phosphate buffer. The resulting phytochrome had a specific absorbance ratio (SAR = A₆₆₆/A₂₈o) ranging from 0.60 to 0.85. These values are equivalent to those of phytochrome preparations after hydroxyla-patite chromatography-ammonium sulfate back-extraction. The total isolation-purification time was 8 h and yield of the chromoprotein was 50% higher than the yield using conventional techniques. The phytochrome preparation, after application to a Toyopearl HW-65S gel filtration column, produced very pure 124 kDa phyA with a specific absorbance ratio greater than 1.00. The spectral characteristics are identical to those described for the best of the highly purified native chromoprotein preparations.     

亲和毛细管电泳法测定牛血清白蛋白和加替沙星的结合常数

利用亲和毛细管电泳法对牛血清白蛋白(BSA)与加替沙星(GT)间的结合反应及其相互作用做了初步探索,并应用淌度比(M)作为指标测定了两者的结合常数。以20 mmol/L pH 7.4的磷酸盐缓冲液作为运行缓冲液,分别以GT和BSA作为添加剂,另一组分为进样样品,内标为二甲基甲酰胺,于214 nm波长下检测。两种测定条件下得到的结合常数分别为4.4×104 L/mol和4.2×104 L/mol,与传统的荧光淬灭法测得的结果基本一致。该方法具有简单、高效的优点。     

高效液相色谱-荧光检测法测定血浆中总同型半胱氨酸

 建立了测定血浆中总同型半胱氨酸的柱前衍生、高效液相色谱-荧光检测的分析方法。以Br omobimane作荧光剂,对巯基进行衍生。同型半胱氨酸的最低检测浓度为0.5 μmol/L,线性 浓度范围是2.5～80.0 μmol/L,回收率为94.0%～112.0%,批内、批间相对标准偏差都小于5. 6%。 关键词：     

高效液相色谱-荧光检测法分析麦类中麦角克列斯汀碱

提出了一种采用高效液相色谱-荧光检测法(HPLC-FLD)测定麦类样品中麦角克列斯汀碱的方法。麦类样品经V(乙腈)∶V(0.1 mol/L乙酸铵缓冲溶液)=1∶4提取,以C18小柱净化,C18色谱柱(4.6×250 mm,5μm)分离,V(水)∶V(乙腈)=3∶2作流动相,流速1.0 mL/min,以HPLC-FLD定量测定。标准工作溶液浓度在1.0~50.0μg/L范围内,与峰面积成良好的线性关系,线性相关系数0.9999,样品在10.0、50.0、250.0μg/kg添加水平的回收率为76%~85%,相对标准偏差(RSD)为6.6%~8.8%(n=8),方法检测限为5.0μg/kg(S/N10)。     

毛细管电泳法测定阿霉素脂质体药物中的微量硫酸根离子

建立了以硝酸钾作为背景电解质测定阿霉素脂质体药物中微量硫酸根离子的毛细管电泳分析法。考察了分离电压、背景电解质、电渗流改性剂浓度、pH值对分离测定的影响。结果表明,当毛细管长度为60 cm(有效长度51.5 cm)、分离电压为~15 kV、缓冲溶液采用20 mmol/L硝酸钾(pH 7.0)、电渗流改性剂采用0.4 mmol/L十六烷基三甲基氯化铵(CTAC)、检测波长为202 nm时,阿霉素脂质体破乳液中硫酸根离子和氯离子在3 min内得到了基线分离,硫酸根离子迁移时间和峰面积的相对标准偏差分别小于0.01%和1.0%,检出限为5 μg/L。用该方法对阿霉素脂质体样品中的微量硫酸根离子进行了分析测定,结果令人满意。