盐酸多赛平与牛血清白蛋白结合反应光谱法研究

用荧光法和紫外-可见光谱法研究了在生理条件下,盐酸多赛平(DH)和牛血清白蛋白(BSA)结合反应的特征。实验发现DH对BSA的荧光有较强猝灭作用,DH的紫外吸收光谱和BSA的荧光光谱有一定程度的重叠现象,20℃反应的结合常数为4.42×104mol/L,DH在BSA分子上色氨酸残基所在区域的结合位点数为1.75,结合反应的标准焓变、标准熵变、标准吉布斯自由能分别为-15.16kJ/mol、36.83J/(mol.K)、-25.95kJ/mol,作用距离r=3.70nm。     

荧光光谱法研究培氟沙星与牛血清白蛋白结合反应特征

在不同温度下,扫描了培氟沙星(PEFL)与牛血清白蛋白(BSA)作用的荧光猝灭光谱、同步荧光光谱、三维荧光光谱和紫外可见吸收光谱,分别用Stern-Volm er方程、L ineweaver-Burk方程和热力学方程等处理实验数据,得到了结合反应的相关参数KLB、ΔHθ、ΔGθ和ΔSθ等的平均值分别为8.419×103L.mol-1、150.7 kJ.mol-1、-22.88 kJ.mol-1和561.9 J.K-1,结合位点数为1.081;证实了在实验浓度和温度范围内,PEFL与BSA可结合形成具有一定结构的复合物,荧光猝灭作用符合静态猝灭作用特征,作用力主要是疏水作用力和静电作用力;为研究PEFL的药理作用和生物学效应,以及PEFL对蛋白质构像的影响等提供了重要信息。     

荧光光谱法研究橙皮甙与牛血清白蛋白的相互作用

在pH7.40的Tris-HCl 缓冲体系中,采用荧光光谱技术研究了橙皮甙与牛血清白蛋白(BSA)的相互作用.随着温度升高,橙皮甙与BSA 的猝灭常数逐渐增加.实验表明:橙皮甙对BSA的荧光猝灭为动态猝灭过程.由热力学参数焓变(△H=70.71 kJ/mol)大于零和熵变[△S=316.29J/(mol·K)]大于零,...     

光谱法研究氨基己酸与牛血清白蛋白的相互作用

利用荧光光谱、紫外-可见吸收光谱及圆二色（CD）光谱研究了模拟生理条件下的氨基己酸(ACA)与牛血清白蛋白(BSA)的相互作用。 实验结果分析表明,氨基己酸对BSA的内源性荧光具有猝灭作用,属于动态猝灭过程。 计算了2种温度下ACA-BSA体系的结合常数、结合位点数及反应的热力学参数ΔG、ΔH和ΔS分别约为-21.00 kJ/mol、-0.64 kJ/mol和-72.00 kJ/(mol·K),由此推出了二者主要通过氢键和范德华力形成摩尔比为1∶1的复合物。 依据Forster非辐射能量转移理论求得二者之间的结合距离为2.3 nm。 位点取代实验指出氨基己酸主要结合在位点Site I。 CD光谱表明,氨基己酸诱导了BSA分子二级结构微变。     

荧光法研究盐酸多赛平与牛血清白蛋白的相互作用

应用荧光共振能量转移(FRET)技术研究了生理条件下,盐酸多赛平(DH)和牛血清白蛋白(BSA)的相互作用.结果表明DH经非辐射能量转移猝灭BSA的荧光.分析荧光猝灭光谱数据,由Stern-Volmer方程、double-reciprocal方程和热力学公式,求得20℃时该反应的标准焓变、标准熵变、标准吉布斯自由能变分别为-15.16kJ.mol-1,37.20J.mol-1.K-1,-26.06kJ.mol-1.结合反应的结合常数为4.42×104,DH在BSA分子上色氨酸残基所在区域的结合位点数为1.75,其作用距离为3.70nm.并从同步荧光光谱考察了DH对BSA构象的影响.     

杂多酸盐K7[PTi2W10O40]·6H2O与人血清白蛋白(HSA)的作用研究

在模拟动物体生理条件下,用荧光和紫外光谱研究了在不同温度下杂多酸盐K7[PTi2W10O40]·6H2O(PM-19)与人血清白蛋白(HSA)结合反应的光谱行为.试验发现,PM-19对HSA有较强的荧光猝灭作用.用Stern-Volmer和Lineweaver-Burk方程分别处理试验数据,发现HSA与PM-19发生反应生成了新的复合物,属于静态荧光猝灭.由Lineweaver-Burk方程求出了不同温度下反应时复合物的形成常数KA(298 K:2.26×105 L/mol;303 K:1.67×105 L/mol;310K:1.01×105 L/mol)及对应温度下结合反应的热力学参数(△H=-51.12kJ/mol;△S=-88.19/-87.85/-88.26 J/K;△G=-24.84/-24.50/-23.78kJ/mol),证明二者之间的主要作用力氢键和范德华力.根据F(o)rster非辐射能量转移机制计算出了两者之间作用距离(4.21 nm).同时用同步荧光光谱法探讨了PM-19对HSA构象的影响.     

罗丹明B与牛血清白蛋白的相互作用研究

在模拟动物体生理条件和不同温度下,用荧光光谱和紫外-可见吸收光谱法研究了罗丹明B(RHB)与牛血清白蛋白(BSA)结合反应的光谱行为.试验发现,RHB对BsA有较强的荧光猝灭作用.用Stern-Volmer和Lineweaver-Burk方程分别处理试验数据,发现BSA与RHB发生反应生成了新的复合物,属于静态荧光猝灭.求出了反应时复合物的形成常数KLB(6.080×104L·mol-1)、热力学参数(△Hθ=-5.997 kJ·mol-1,△Sθ=72.01 J·k-1,△Gθ=-28.09kJ·mol-1)和结合位点数(1.025)等,证明二者主要靠静电作用力结合.同时用同步荧光光谱及三维荧光光谱法探讨了RHB对BSA构象的影响,表明RHB使色氨酸残基所处微环境的极性减弱、疏水作用增强.为阐明RHB的染色机理、毒理效应和生物学效应提供了重要信息.     

溴百里酚蓝与牛血清白蛋白的相互作用研究

在模拟动物体生理条件和不同温度下,用荧光光谱和紫外-可见吸收光谱法研究了溴百里酚蓝(BTB)与牛血清白蛋白(BSA)结合反应的光谱行为。用Stern-Volmer和Lineweaver-Burk方程分别处理试验数据,发现BSA与BTB发生反应生成了新的复合物,属于静态荧光猝灭。求出了反应时复合物的形成常数KLB(2.792×105L.mol-1)、热力学参数(ΔHθ=(20.24 kJ.mol-1,ΔSθ=37.22J.K-1,ΔGθ=(31.25kJ.mol-1)与结合位点数(1.1578)。根据F rster偶极-偶极非辐射能量转移理论计算出结合位置距离212位色氨酸残基2.60nm,证明二者主要靠静电作用力结合。同时用同步荧光光谱和三维荧光光谱法探讨了BTB对BSA构象的影响,表明BTB使色氨酸残基所处微环境的极性减弱、疏水作用增强,为阐明BTB的染色机理、毒理效应和生物学效应提供重要信息。     

荧光光谱法研究氰戊菊酯与牛血清白蛋白的结合反应

在生理酸度条件下,应用荧光光谱法和紫外一可见光谱法,研究了在不同温度下氰戊菊酯与牛血清白蛋白（BSA）的结合反应。实验发现,氰戊菊酯对BSA的内源荧光有较强的猝灭作用,且此荧光猝灭机理为动态猝灭。由热力学参数焓变△H=17.78kJ·mol^-1,熵变△S=143.65J·mol^-1·K^-1,推断出氰戊菊酯与BSA结合反应主要由疏水作用力驱动。根据Fǒrster非辐射能量转移理论,计算出供体（BSA）-受体（氰戊菊酯）间的结合距离为4.28nm,能量转移效率为0.0699。三维荧光光谱表明氰戊菊酯的存在使BSA的构象发生了变化。此外,还考察了某些金属离子对氰戊菊酯与BSA结合反应的影响。     

川陈皮素与牛血清白蛋白相互作用的光谱研究

用多种光谱技术研究了生理条件下川陈皮素(NOB)与牛血清白蛋白(BSA)的相互作用及热力学特征.结果表明,NOB与BSA有较强的作用,NOB能使BSA的内源荧光猝灭,并以静态猝灭为主.按照Stern-Volmer方程和双对数方程分别得出不同温度下,以及不同pH值时NOB与BSA的结合常数和结合位点数.运用紫外光谱获得常温下NOB与BSA的结合常数与荧光光谱测定值相近,热力学参数△H、△S分别为55.91 kJ·5moL~(-1)、274.61 J·5moL~(-1)·5K-1,表明其主要作用力为疏水力,NOB与BSA作用为非辐射能量转移机制,其能量转移效率与结合距离分别为0.27和1.76 nm,用参比法得出BSA荧光量子产率为0.074.同步荧光光谱研究发现川陈皮素对牛血清白蛋白构象几乎没有影响.     

铜(Ⅱ)离子与神经红蛋白的相互作用

利用紫外可见吸收光谱、荧光光谱、同步荧光光谱及圆二色(CD)光谱研究了铜髤离子与神经红蛋白(NGB)的相互作用。结果表明,Cu2+离子使NGB在280nm处的紫外吸收增强,说明Cu2+与NGB发生了相互作用;Cu2+使NGB内源性荧光发生猝灭,其猝灭机制为静态猝灭;同步荧光光谱表明,Cu2+使色氨酸微环境的疏水性有所降低,Cu2+对NGB的作用位点更接近于色氨酸;CD光谱显示Cu2+没有引起NGB二级结构明显的变化。     

碳笼氧化物的结构和谱学性质

碳笼氧化物对碳笼的官能团化研究具有重要意义，因而激起了人们广泛的研究兴趣。本文对碳笼氧化物C₆₀O_n、C₇₀O_n、C₇₆O_n、C₇₈O_n及C₈₀O_n的结构、电子光谱、红外光谱及核磁共振谱的研究进展进行综合评述。介绍了国内外近十几年来众多研究小组的工作，并结合作者本人在此方面的理论研究成果，进一步探索碳笼氧化物的结构特点以及光谱性质的规律性。在对C₆₀及C₇₀氧化物研究结果与实验符合的基础上，预测高碳笼氧化物的结构和光谱。     

Synthesis and structural studies of complexes of Co(II), Ni(II), Cu(II), Zn(II) and Cd(II) with substituted chalcones

Complexes of Co(II), Ni(II), Cu(II), Zn(II) and Cd(II) with 3-(2-pyridyl)-1-(2-hydroxy phenyl)-2-propen-1-one (PHPO), 3-(1-naphthyl)-1-(2-hydroxy phenyl)-2-propen-1-one (NHPO) and 3-(3,4-dimethoxy phenyl)-1-(2-hydroxy phenyl)-2-propen-1-one (DMPHPO) have been synthesized and characterized by analytical, conductivity, thermal, magnetic, infrared, electronic and electron spin resonance data. Based on analytical data the stoichiometry of the complexes has been found to be 1 : 2. The conductivity data show that all these complexes are non-electrolytes. The infrared spectral data indicate that the ligand PHPO acts as uninegative tridentately towards Co(II) and Ni(II) and bidentately with Cu(II), Zn(II) and Cd(II). Ligands like NHPO and DMPHPO act as uninegative bidentately with all the metal ions. The electronic spectral data suggest that all the Co(II) complexes and Ni(II) of PHPO complex are octahedral and all the Cu(II) and Ni(II) of NHPO and DMPHPO complex are square-planar. The complex of Zn(II) and Cd(II) are tetrahedral. ESR parameters of Cu(II) complexes have been calculated and relevant conclusions have been drawn with respect to the nature of bonds present in them.     

Chlorofluorieren mit Trifluormethylhypochlorit CF3OCl. Darstellung und spektroskopische Charakterisierung von Trifluormethyliodchloridfluorid CF3I(Cl)F

Chlorofluorinate with Trifluoromethylhypochlorite CF₃OCl. Preparation and Spectroscopic Characterization of Trifluormethyliodinechloridefluoride CF₃I(Cl)F The preparation of a new iodine(III) compound, trifluormethyliodinechloridefluoride CF₃I(Cl)F, via oxidative addition of trifluoromethylhypochlorite CF₃OCl to trifluoromethyliodide CF₃I is described. The thermolabile compound has been characterized by i.r., Raman, ¹⁹F NMR, and mass spectroscopy.     

5,10,15,20-四(对氯苯基)卟啉羟基稀土配合物的光谱研究

研究了四(对氯苯基)卟啉及其羟基稀土配合物的紫外-可见光谱、红外光谱和拉曼光谱,对主要谱带进行了经验归属,讨论了中心离子对紫外光谱和共振拉曼光谱结构敏感带频率的影响.     

Derivate von Tricyanmethan. 2. Untersuchungen über Methyltricyanmethan (1,1,1-Tricyanethan) [1]

Derivatives of Tricyanomethane. 2. Studies on Methyl Tricyanomethane (1,1,1-Tricyanoethane) The title compound H₃C? C(CN)₃ has been characterized by means of nuclear magnetic resonance, vibrational, and mass spectra.     

Diffuse reflection FTIR spectral database of dyes and pigments

24 pigments commonly used in art have been characterized by diffuse reflection infrared spectroscopy (DR). All of the compounds have also been characterized by means of infrared absorption spectroscopy to demonstrate the reliability of the DR technique. This is the first record of the use of this technique as an analytical tool in conservation science, and the results appear to be promising for the identification of unknown pigments used on historical and artwork artifacts. Although the DR technique used here is not nondestructive, it can still be usefully applied to the analysis of artwork since it requires only a very small quantity of sample for analysis.     

The role of TiO2 in the B2O3–Na2O–PbO–Al2O3 glass system

Optical and vibrational studies have been carried out on 60B₂O₃·(20−x)Na₂O·10PbO·10Al₂O₃:xTiO₂ (x=0, 1, 2, 3, 4, and 5 mol%) glasses, in order to understand the role of TiO₂ in the 60B₂O₃·20Na₂O·10PbO·10Al₂O₃ glass matrix. The X-ray patterns reveal homogeneous glasses over the entire compositional range. The absorption spectra show that the energy of the optical band gap (ΔE_opt) and Urbach's energy (E_U) decreases as TiO₂ content increases. The changes observed in the Raman and IR spectra are related to the BO₄→BO₃ back conversion effect and the appearance of “loose” BO₄⁻ groups. The data indicate that titanium ions act as a network modifier.     

Dinitramide and its salts

The vibrational and electronic spectra of dinitramide salts MN(NO₂)₂ (M=K, Na, Li, NH₄, Fe, Ag, Mn, Mg, Rb, C(NH₂)₃) have been studied. The IR and Raman spectra of solutions and melts of the salts have been satisfactorily interpreted on the basis of the anion model withC _2v symmetry. The complication of the spectra in the crystalline phase has been explained by restructurization of the anion that reduces its symmetry and makes the nitro groups nonequivalent.For part 6, seeIzv. Akad. Nauk, Ser. Khim., 1995, 113 [Russ. Chem. Bull.,44, 108 (Engl. Transl.)].Translated fromIzvestiya Akademii Nauk. Seriya Khimicheskaya, No. 8, pp. 1508–1512, August, 1995.     

Preparation and investigation of the crystals formed in the buckminsterfullerene-tetraselenotetracene-carbon disulfide system

Crystals containing up to 30 wt. % CS₂, according to the data of IR and X-ray photoelectron spectroscopy, were isolated from the C₆₀-TSeT-CS₂ (TSeT is tetraselenotetracene) system. The unusually high concentration of carbon disulfide results in the complete sublimation of the crystals at a relatively high temperature (520 °C). The electron energy loss spectra of the crystals obtained were measured and analyzed.Translated fromIzvestiya Akademii Nauk. Seriya Khimicheskaya, No. 5, pp. 845–848, May, 1995.This work was financially supported by the Russian Foundation for Basic Research (Projects No. 93-03-18002, 93-03-18705, and 93-03-5650).