提高拮抗菌WB3对番茄灰霉病菌拮抗能力的He-Ne激光与氯化锂复合诱变技术

利用He-Ne激光(波长632.8 nm,功率12 mW)与氯化锂复合诱变一株番茄灰霉病菌拮抗菌WB3,获得14株拮抗效果突出的菌株,其最大抑菌带宽为15.7 mm.综合单一诱变组里菌株致死率以及拮抗能力的大小,其中辐照时间为30 min,氯化锂浓度为1.2%时为复合诱变最佳条件.经过复合诱变,L8拮抗菌最高抑菌带宽可提高14%以上,而单一He-Ne激光诱变后抑菌带宽提高8.8%,单一氯化锂诱变后抑菌带宽提高9%.L8菌株生长迅速,经传代培养证明其抑菌性能具有稳定遗传性.该实验研究He-Ne激光与氯化锂复合诱变枯草芽孢杆菌WB3的方法可应用于番茄灰霉病的生物防治领域.     

激光应用 激光生物学

Q631 2005064123 碱性淀粉酶菌株的紫外线和He-Ne激光复合诱变及其产 酶条件的研究=Study on the breeding of alkaline amylase producing strain bv UV and He-Ne laser compound muta- tion and the conditions for enzyme production[刊,中]/刘 建龙(华南理工大学食品与生物学院．广东,广州 (510641)),刘建军…∥激光杂志．-2005,26(3)．-83-84 以嗜碱芽胞杆菌BC-A36为出发菌株,通过紫外线     

提高拮抗菌WB3对番茄灰霉病菌拮抗能力的He-Ne激光与氯化锂复合诱变技术

利用He-Ne激光(波长632.8 nm,功率12 mW)与氯化锂复合诱变一株番茄灰霉病菌拮抗茵WB3,获得14株拮抗效果突出的菌株,其最大抑菌带宽为15.7 mm.综合单一诱变组里菌株致死率以及拮抗能力的大小,其中辐照时间为30 min,氯化锂浓度为1.2%时为复合诱变最佳条件.经过复合诱变,L8拮抗茵最高抑菌带宽可...     

He-Ne激光诱变原生质体选育四环素高产菌的研究

采用He-Ne激光对金霉素链霉菌(S.aureofaciens)原生质体诱变处理,结果表明:He-Ne激光对该菌原生质体具有明显的致死作用.经激光处理的原生质体再生株产抗生素能力与亲本菌株相比,四环素产量平均提高9.32%,最高提高20.6%.     

激光诱变选育面包酵母菌的研究

采用He-Ne激光对面包酵母菌进行诱变处理,筛选到3株低糖面团起发活力大幅度提高的菌株XD198、XD193和XD174,其低糖面团起发活力比出发菌株分别提高31.3%、32.7%和35.3%.海藻糖含量分别较出发菌株提高-1.37%、10.83%、5.10%.而且筛选到的高活力菌株遗传性能稳定.证明激光诱变效果明显,是进行面包酵母菌种选育的理想方法之一.     

紫外、氦氖激光等复合诱变产果胶酶细菌ZH1的研究

ZH₁菌株是从自然界分离筛选而来的,该菌株产果胶酶的最适pH为7.0;最适温度33℃;培养36h达产酶高峰,酶活力为73.5μ/mL,将ZH₁作为出发菌株,经紫外线诱变、硫酸二乙酯诱变,亚硝基胍和紫外线复合诱变及氦氖激光等多次反复诱变,选育得到一株产果胶酶性稳定且酶活明显提高的突变株ZHg,其酶活为301m/mL,比出发菌株ZH₁产果胶酶能力提高3.1倍.     

激光诱变选育果胶酶高产菌株

采用Ar⁺、He-Ne、LD、YAP等激光辐照果胶酶产生菌,不同波长激光辐照均获高酶活菌株,其中从LD激光与He-Ne激光辐照组中选育出酶活达6×10⁴单位/g的高产菌株(比对照组提高122%),并稳定地应用于生产.文中从黑曲霉抱子与DNA的吸收光谱以及电镜观察黑曲霉抱子的结果出发,对激光诱变机制进行了初步探讨.     

He-Ne激光对γ-亚麻酸产生菌少根根霉的诱变作用

采用He-Ne激光和化学诱变剂LiCl对少根根霉孢子和原生质体进行复合诱变,从一株产GLA的出发菌株少根根霉R8#中选育出高产菌株RC378.摇瓶培养含油脂量47.8%,GLA含量12.6%,比出发菌株油脂含量提高了130.8%,GLA含量提高了276.7%.从菌落形态、同工酶酶谱、红外光谱吸收特征和油脂产量及组成比较分析表明:该菌株较出发菌株具有显著的变异.菌株RC378经传代培养,产脂量维持在41.1%～48.7%,GLA含量维持在10.8～12.6%.该菌株具有较好的遗传稳定性.     

He-Ne激光对钝顶螺旋藻的诱变效应

利用He-Ne激光(波长632 nm，功率10 mW)诱变钝顶螺旋藻IS（Spirulina platensis IS），经过一定时间的辐照，选取生长较快的藻种测量其β-胡萝卜素、蛋白质及多糖含量，进一步筛选出生长快、高产胡萝卜素或高产蛋白质的藻种.实验结果表明：与出发藻种IS相比，经He-Ne激光15min和25 min照射后的藻种MS-1、MS-2和 MS-3，藻丝形态发生变化，藻丝变短、螺旋变紧密，生长速度明显加快，其中 MS-1的β-胡萝卜素含量增幅为18.1%，MS-3的蛋白质和多糖含量均有较大增加.此外通过对出发藻种和诱变藻种的叶绿素a和胡萝卜素的紫外吸收光谱进行比较，发现诱变藻种与出发藻种相比最大吸收峰值略有变化，从而说明He-Ne激光对于钝顶螺旋藻的诱变效应.     

紫外激光等选育生淀粉糖化酶高产菌株及其应用

以紫外线、LiCl、5-氟尿嘧啶、HNO2为诱变剂,黑曲霉Sx为出发菌株,选育出生淀粉糖化酶酶活力提高12%的突变株黑曲霉S′x.制取黑曲霉S′x的原生质体,用He-Ne激光照射,经反复筛选得到突变株黑曲霉Sy,其酶活力较出发菌株提高51%,而酸性蛋白酶活力降低45%.并对其在农作物秸杆的应用进行了研究.     

Study of Laser-induced Mutation on Gibberellin-produced Strains

In this ppaer, it was presented that after Gibberellin-produced strains were irradiated with He-Ne laser for 10～15 minutes, high yield strains, obtained by continuous screening, increased potency (unit) by over 110% than that of control strains. More high yield strains were also obtained by the combined treatment with He-Ne laser and LiCl. The mutagenic effects were more exciting because of potency improvement rate reached 189.6%. It seemed that the effect of the combined treatment was better than that of the single one. The experimental results indicated that is important to choose suitable wavelengths dosages and power density of laser and mutagenic condition for obtaining high yield strains.     

Study of Laser induced Mutation on Gibberellin produced Strains

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He-Ne激光选育高木质素降解率的白腐真菌Lx

用He-Ne激光对一株木质素降解活性较高的白腐真菌L₁进行选育.将该菌的菌丝体和原生质体多次照射诱变,结果得出:在波长632.5nm,用功率为6mW和7mW照射该菌的菌丝体,选育出的菌株L₆和L₇木质素降解率可达38.1%和39.88%,比出发菌株L₁提高了33%和39%.用功率9mW的He-Ne激光辐照原生质体,照射20min时,原生质体致死率已达100%.在照射时间为10min时,选育出一株木质素降解率达43.03%的菌株L_x,比出发菌株L₁提高了50%.同工酶分析显示该菌株发生了稳定的遗传突变.     

氦-氖激光诱变细菌细胞及原生质体选育果胶酶高产菌株

利用氦-氖激光(波长632.8nm,功率15mW)诱变果胶酶产生菌的菌体细胞,获得了突变株ZH-g,其酶活达到301 u·mL－1,是出发菌株的1.3倍.进一步用氦-氖激光照射ZH-g的原生质体,从而得到了高产突变株ZH-gA,其酶活达到357 u·mL－1,比ZH-g提高了18%,是出发菌株的1.6倍.该菌株在好氧和静置条件下均能良好产酶,经传代实验证明其产酶性能稳定.     

Displacement sensor based on polarization mixture of orthogonal polarized He-Ne laser at 1.15μm

Displacement sensor based on the polarization mixture and the cavity tuning of the orthogonal polarized He-Ne laser 1.15μm is presented.The power tuning curves of He-Ne laser are irregular,and it is difficult to measure the change in cavity length.The distortion of the curves is caused by the higher relative excitation compared with the He-Ne laser at 633 nm.In view of its potential for the wider displacement measuring range,a new method of displacement sensing is developed.Experiments show that displacement measuring stability based on the method of the polarization mixture is better than that of the power tuning curves. The displacement sensor achieves the measuring range of 100 mm,resolution of 144 nm,and linearity of 7×10-6 .     

Photodegradation of melanin by an interferometric technique

Photodegradation of melanin thin films is investigated for a UVA wavelength of 355 nm and a UVC wavelength of 244 nm. The technique involves interferometric exposure of melanin with two coherent beams from a low-power UV laser. The periodic photodegradation-grating pattern is monitored by diffraction of a second low-power He-Ne laser. Dependence of the photodegradation rate on UV intensity as well as the effect of ambient humidity is investigated and explained with a simple model. The technique has promise for investigating photo-induced effects in other biomolecular substrates as well.     

He-Ne激光对小麦幼苗增强UV-B辐射损伤修复的影响

采用He-Ne激光(5mW/mm²)来辐照处理经增强UV-B(10.8kJ·m^-2·d^-1)辐射损伤的小麦幼苗,通过荧光光谱测定其中双链DNA(dsDNA)的含量,分析研究了He-Ne激光对小麦DNAUV-B损伤的切除修复的影响和机制,以探明激光对UV-B损伤的修复途径及机制.结果表明:He-Ne激光能明显增强UV-B辐射处理后小麦种子的萌发力;小麦对UV-B辐射损伤具有一定的切除修复能力,切除修复的高峰期发生在UV-B辐射后4～6h内;He-Ne激光主要通过促进小麦的切除修复途径影响小麦对UV-B损伤的修复,而且能增强小麦的切除修复能力,其促进作用在修复高峰期(5h)表现尤为明显.     

Cancellation of laser dither modulation from optical frequency standards

We demonstrate the removal of the dither modulation from an iodine-stabilized He-Ne laser by using a frequency-modulated acousto-optic modulator and feed-forward techniques. This procedure reduces the linewidth of the beat between this laser and a flywheel He-Ne laser from 6 MHz to 8 kHz, the undithered beat linewidth being ~7 kHz. Dither suppression greatly reduces counter errors during beat measurements from stroboscopic effects between the counter's gate and the frequency of the dither modulation and increases the utility of the already formidable array of dithered laser frequency standards by making locking to them an easier task.     

He-Ne激光器放电点火电压的实验研究

寻找能降低He—Ne激光器点火电压的外加催离剂．实验对He—Ne激光器的点火电压在阴、阳极被可见光照射、低放射源作用于放电管和阴极附近加强电场的条件下进行了测量．对不同外加条件作用下He—Ne激光器点火电压实验数据的误差进行了分析．实验结果指出，用可见光照射阴极或阳极不能降低He—Ne激光器的点火电压；能明显降低点火电压的因素可以是低放射源或外加强电场．