| 氦-氖激光诱变细菌细胞及原生质体选育果胶酶高产菌株 |
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| 引用本文： | 朱宏莉,郭爱莲,宋纪蓉,杨秀芳,张嘉,张晓瑞.氦-氖激光诱变细菌细胞及原生质体选育果胶酶高产菌株[J].光子学报,2005,34(11):1693-1696. |
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| 作者姓名： | 朱宏莉 郭爱莲 宋纪蓉 杨秀芳 张嘉 张晓瑞 |
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| 作者单位： | 西北大学生命科学学院,西安,710069;西北大学化工学院,西安,710069;西北大学生命科学学院,西安,710069;西北大学化工学院,西安,710069;西安理工大学机械与精密仪器工程学院,西安,710048 |
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| 基金项目： | 陕西省教委基金资助项目（00JK138）. |
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| 摘 要： | 利用氦-氖激光(波长632.8nm，功率15mW)诱变果胶酶产生菌的菌体细胞，获得了突变株ZH-g，其酶活达到301 u·mL－1，是出发菌株的1.3倍.进一步用氦-氖激光照射ZH-g的原生质体，从而得到了高产突变株ZH-gA，其酶活达到357 u·mL－1，比ZH-g提高了18%，是出发菌株的1.6倍.该菌株在好氧和静置条件下均能良好产酶，经传代实验证明其产酶性能稳定.
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| 关 键 词： | 果胶酶 细菌 He-Ne激光 原生质体 诱变 |
| 收稿时间： | 2005-04-01 |
| 修稿时间： | 2005年4月1日 |
The Breeding of High-Yield Pectinase Producing Bacteria Using He-Ne Laser Irradiation on the Cell and Protoplasts |
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| Zhu Hongli,Guo Ailian,Song Jirong,Yang Xiufang,Zhang Jia,Zhang Xiaorui.The Breeding of High-Yield Pectinase Producing Bacteria Using He-Ne Laser Irradiation on the Cell and Protoplasts[J].Acta Photonica Sinica,2005,34(11):1693-1696. |
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| Authors: | Zhu Hongli Guo Ailian Song Jirong Yang Xiufang Zhang Jia Zhang Xiaorui |
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| Institution: | 1 Institute of Life science,Northwest University,Xi′an 710069;2 Institute of chemistry Engineering,Northwest University,Xi′an 710069 |
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| Abstract: | A mutant strain ZH-g was screened by He-Ne laser mutagenesis on the cells.Its pectinase activity was in 1.3 fold of the original one,reaching 301 μ/mL.On the basis of this,using He-Ne laser irradiating on the protoplasts of ZH-g,the mutant strain ZH-gA with high yield of pectinase was obtained.It accumulated pectinase activity up to 357 μ/mL which was 18% higher than that of the parent strain.This strain can produce pectinase efficiently under both aerobic and static cultivation.The enzyme activity is stable after several times of batch. |
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| Keywords: | Pectinase Bacteria He-Ne laser Protoplast Mutagenesis |
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