PIV measurements of a microchannel flow

 A particle image velocimetry (PIV) system has been developed to measure velocity fields with order 1-μm spatial resolution. The technique uses 200 nm diameter flow-tracing particles, a pulsed Nd:YAG laser, an inverted epi-fluorescent microscope, and a cooled interline-transfer CCD camera to record high-resolution particle-image fields. The spatial resolution of the PIV technique is limited primarily by the diffraction-limited resolution of the recording optics. The accuracy of the PIV system was demonstrated by measuring the known flow field in a 30 μm×300 μm (nominal dimension) microchannel. The resulting velocity fields have a spatial resolution, defined by the size of the first window of the interrogation spot and out of plane resolution of 13.6 μm× 0.9 μm×1.8 μm, in the streamwise, wall-normal, and out of plane directions, respectively. By overlapping the interrogation spots by 50% to satisfy the Nyquist sampling criterion, a velocity-vector spacing of 450 nm in the wall-normal direction is achieved. These measurements are accurate to within 2% full-scale resolution, and are the highest spatially resolved PIV measurements published to date. Received: 29 October 1998/Accepted: 10 March 1999     

Turbulent flow measurement in a square duct with hybrid holographic PIV

 A hybrid holographic system has been developed for three-dimensional particle image velocimetry. With unique high pass filters, the system combines advantages of both in-line and off-axis holography without having their draw-backs. It improves the signal to noise ratio of the reconstructed image, allows use of 3–15 μm particles in water at high population and achieves large dynamic ranges in both velocity and space. With an automated image acquisition and processing system it has been used for measuring the velocity distributions in a square duct at Re=1.23×10⁵. The data consists of 97×97×87 vectors (with 50% overlapping of adjacent interrogation windows). The quality of the results is evaluated using the continuity equation. The deviation from the equation decreases rapidly with increasing control volume and reaches a level of less than 10%. Mean velocities, r.m.s. velocity fluctuations and turbulence spectra are estimated using the data. Received: 16 December 1996/Accepted: 6 March 1997     

Experimental investigation on turbulence modification in a horizontal channel flow at relatively low mass loading

Particle-laden flows in a horizontal channel were investigated by means of a two-phase particle image velocimetry (PIV) technique. Experiments were performed at a Reynolds number of 6 826 and the flow is seeded with polythene beads of two sizes, 60 μm and 110 μm. One was slightly smaller than and the other was larger than the Kolmogorov length scale. The particle loadings were relatively low, with mass loading ratio ranging from 5×10⁻⁴ to 4×10⁻² and volume fractions from 6×10⁻⁷ to 4.8×10⁻⁵, respectively. The results show that the presence of particles can dramatically modify the turbulence even under the lowest mass loading ratio of 5×10⁻⁴. The mean flow is attenuated and decreased with increasing particle size and mass loading. The turbulence intensities are enhanced in all the cases concerned. With the increase of the mass loading, the intensities vary in a complicated manner in the case of small particles, indicating complicated particle-turbulence interactions; whereas they increase monotonously in the case of large particles. The particle velocities and concentrations are also given. The particles lag behind the fluid in the center region but lead in the wall region, and this trend is more prominent for the large particles. The streamwise particle fluctuations are larger than the gas fluctuations for both sizes of particles, however their varying trend with the mass loadings is not so clear. The wall-normal fluctuations increase with increasing mass loadings. They are smaller in the 60 μm particle case but larger in the 110 μm particle case than those of the gas phase. It seems that the small particles follow the fluid motion to certain extent while the larger particles are more likely dominated by their own inertia. Finally, remarkable non-uniform distributions of particle concentration are observed, especially for the large particles. The inertia of particles is proved to be very important for the turbulence modification and particles behaviors and thus should be considered in horizontal channels. The project supported by the National Natural Science Foundation of China (50276021), and Program for New Century Excellent Talents in University, Ministry of Education (NCET-04-0708) The English text was polished by Yunming Chen.     

Microscale 3D flow mapping with μDDPIV

Three-dimensional (3D) quantitative flow visualization by tracking microscale particles has become an invaluable tool in microfluid mechanics. Defocusing digital particle image velocimetry (DDPIV) can recover spatial coordinates by calculating the separation between defocused images generated by an aperture mask with a plurality of pinholes. In this paper, a high-speed 3D micro-DDPIV (μDDPIV) system was devised based on this technique to achieve microscale velocity field measurements. A micro-volume of 400 × 300 μm² with a depth of 150 μm has been mapped using an inverted microscope equipped with a 20× objective lens. The proposed technique was successfully applied to 3D tracking of 2-μm fluorescent particles inside an evaporating water droplet.     

Application of the PIV technique to measurements around and inside a forming drop in a liquid–liquid system

A particle image velocimetry (PIV) method has been developed to measure the velocity field inside and around a forming drop with a final diameter of 1 mm. The system, including a microscope, was used to image silicon oil drops forming in a continuous phase of water and glycerol. Fluorescent particles with a diameter of 1 μm were used as seeding particles. The oil was forced through a 200 μm diameter glass capillary into a laminar cross-flow in a rectangular channel. The velocity field was computed with a double-frame cross-correlation function down to a spatial resolution of 21 × 21 μm. The method can be used to calculate the shear stress induced at the interface by the cross-flow of the continuous phase and the main forces involved in the drop formation process.     

A calibration procedure for millimeter-scale stereomicroscopic particle image velocimetry

A stereomicroscopic particle image velocimetry (SμPIV) system has been developed for millimeter scale flows. The SμPIV system is based on an off-the-shelf stereomicroscope, with magnification between 0.69× and 30×, and a field of view between 7.5 × 6 mm and 250 × 200 μm. Custom calibration targets were devised using printed circuit board technology, and applied at a magnification factor of 1.74, with a field of view of 4.75 × 3.8 mm. Measurement errors were assessed by moving a test block with fixed particles. Total system uncertainty in test block displacement transverse to the optical axis was 0.5% of the field of view, and 3% of the depth of field for motion along the optical axis. Approximately 20% of this uncertainty was due to the calibration target quality and test block procedures.     

A particle image velocimetry system for microfluidics

 A micron-resolution particle image velocimetry (micro-PIV) system has been developed to measure instantaneous and ensemble-averaged flow fields in micron-scale fluidic devices. The system utilizes an epifluorescent microscope, 100–300 nm diameter seed particles, and an intensified CCD camera to record high-resolution particle-image fields. Velocity vector fields can be measured with spatial resolutions down to 6.9×6.9×1.5 μm. The vector fields are analyzed using a double-frame cross-correlation algorithm. In this technique, the spatial resolution and the accuracy of the velocity measurements is limited by the diffraction limit of the recording optics, noise in the particle image field, and the interaction of the fluid with the finite-sized seed particles. The stochastic influence of Brownian motion plays a significant role in the accuracy of instantaneous velocity measurements. The micro-PIV technique is applied to measure velocities in a Hele–Shaw flow around a 30 μm (major diameter) elliptical cylinder, with a bulk velocity of approximately 50 μm s^-1. Received: 26 November 1997/Accepted: 26 February 1998     

Effect of resolution on the speed and accuracy of particle image velocimetry interrogation

Particle image velocimetry incorporates a process by which an image of a flow field, bearing double images of seeding particles, is analyzed in small regions called “interrogation spots.” Each spot is imaged onto a photodetector array whose digitized output is evaluated computationally using the auto-correlation technique. This paper examines the effects of resolving the spot using arrays of various resolutions, motivated primarily by a gain in speed. For this purpose, two specially created test photographs representing (i) uniform flow and (ii) solid body rotation, were interrogated using array sizes ranging from 32 × 32 to 256 × 256. Each reduction in resolution by a factor of two gains a factor of four in interrogation speed, but this benefit is counteracted by a loss in accuracy. The particle image diameter strongly influences accuracy through two distinct error mechanisms. When the particle image is small compared to the pixel size, mean bias error becomes significant due to finite numerical resolution of the correlation function. Conversely, when the particle image is large, random error due to irregularities in the electronic images predominates. The optimum image size, therefore, lies not at either extreme but at an intermediate value such that the particle image is small in an absolute sense, and yet large relative to the pixel size. A version of this paper was presented at the 12th Symposium on Turbulence, University of Missouri-Rolla, 24–26 September 1990     

Stereoscopic micro particle image velocimetry

A stereoscopic micro-PIV (stereo-μPIV) system for the simultaneous measurement of all three components of the velocity vector in a measurement plane (2D–3C) in a closed microchannel has been developed and first test measurements were performed on the 3D laminar flow in a T-shaped micromixer. Stereomicroscopy is used to capture PIV images of the flow in a microchannel from two different angles. Stereoscopic viewing is achieved by the use of a large diameter stereo objective lens with two off-axis beam paths. Additional floating lenses in the beam paths in the microscope body allow a magnification up to 23×. The stereo-PIV images are captured simultaneously by two CCD cameras. Due to the very small confinement, a standard calibration procedure for the stereoscopic imaging by means of a calibration target is not feasible, and therefore stereo-μPIV measurements in closed microchannels require a calibration based on the self-calibration of the tracer particle images. In order to include the effects of different refractive indices (of the fluid in the microchannel, the entrance window and the surrounding air) a three-media-model is included in the triangulation procedure of the self-calibration. Test measurement in both an aligned and a tilted channel serve as an accuracy assessment of the proposed method. This shows that the stereo-μPIV results have an RMS error of less than 10% of the expected value of the in-plane velocity component. First measurements in the mixing region of a T-shaped micromixer at Re = 120 show that 3D flow in a microchannel with dimensions of 800 × 200 μm² can be measured with a spatial resolution of 44 × 44 × 15 μm³. The stationary flow in the 200 μm deep channel was scanned in multiple planes at 22 μm separation, providing a full 3D measurement of the averaged velocity distribution in the mixing region of the T-mixer. A limitation is that this approach requires a stereo-objective that typically has a low NA (0.14–0.28) and large depth-of-focus as opposed to high NA lenses (up to 0.95 without immersion) for standard μPIV.     

Design and fabrication of a micro thermal actuator for cellular grasping

The development of a novel polymer-based micro robotic gripper that can be actuated in a fluidic medium is presented in this paper. Our current work is to explore new materials and designs for thermal actuators to achieve micromanipulation of live biological cells. We used parylene C to encapsulate a metal heater, resulting in effectively a tri-layered thermal actuator. Parylene C is a bio-compatible dielectric polymer that can serve as a barrier to various gases and chemicals. Therefore, it is suitable to serve as a thermal/electrical/chemical isolation material for protecting the metal heater from exposing to an aqueous environment. We have demonstrated parylene actuators(2 mm×100/μm×0.5μm) to operate in an aqueous environment using 10 to 80 mW input power. The temperature of these actuators at full deflection was estimated to be～ 60℃, which is much lower than the typical requirement of > 100℃ to actuate other conventional MEMS actuators. Danio rerio follicles in fluidic medium were captured successfully using these actuators. Moreover, these actuators were found to be responsive to moderate rise in environmental temperature, and hence, we could vary the fluidic medium temperature to actuate trimorphs on a chip without any input of electrical energy, i.e., raising the fluidic temperature from 23℃ to 60℃ could actuate the trimorphs to grasp follicles of～1mm size in diameter. At 60℃, the embryos inside the follicles were observed to be alive, i.e., they were still moving in the biological fluid isolated by the follicle membrane. The smallest follicles grasped were～500μm in diameter using 800μm×130μm×0.6μm actuators. The fabrication process, modeling, and optimization of the trimorph actuators are presented. Based on the successful operation of these polymer-based actuators, we are currently developing multifinger thermal microgrippers for cellular grasping and manipulation, which can potentially be hybridly integrated with circuits for computer control.     

Particle tracer response across shocks measured by PIV

The experimental approach used for the evaluation of the particle response time across a stationary shock wave is assessed by means of PIV measurements. The study focuses on the experimental requirements for a reliable and unbiased measurement of the particle response time τ _p and length ξ _p based on a single-exponent decaying law. A numerical simulation of the particle response experiment returns the parameters governing the measurement: namely the normalized spatial and temporal resolution, shock strength, and digital resolution. Representing the velocity decay in logarithmic coordinates it is shown that measurements performed with laser pulse separation time up to τ _p and interrogation window up to ξ _p still yield unbiased results for the particle response. A set of experiments on the particle response across a planar oblique shock wave was conducted to verify the results from the numerical assessment. Liquid droplets of DEHS and solid tracer particles of silicon and titanium dioxide with different primary crystal size are compared. The resulting temporal response ranges from 2 to 3 μs, corresponding to values commonly reported in literature, to almost 0.3 μs when particles are properly dehydrated and a filter is applied before injection into the wind tunnel. It is the first experimental evidence of particle tracers with a measured response time lower than 0.4 μs. The same procedure is applied to attempt the measurement of individual particle tracers by particle tracking velocimetry to estimate the spread in the distribution of tracer time response. The latter analysis is limited by the particle image tracking precision error, which biases the results introducing a wider broadening of the particle velocity distribution.     

Simultaneous two-phase PIV by two-parameter phase discrimination

 A flexible and robust phase discrimination algorithm for two-phase PIV employs second-order intensity gradients to identify objects. Then, the objects are sorted into solids and tracers according to parametric combinations of size and brightness. Solids velocities are computed by tracking, gas velocities by cross-correlation. Tests in a fully-developed turbulent channel flow of air showed that the two phases do not contaminate or bias each other's velocity statistics. Error magnitude and valid data yield were quantified with artificial images for three particle sizes (25, 33, and 63 μm), two interrogation area sizes (32 and 64 pixels), and volumetric solids loads from 0.0022% to 0.014%. At the channel centerline, the gas valid data yield was above 98% and the RMS error in gas velocity was less than 0.1 pixels for all variations of these parameters. The solid-to-tracer signal ratio was found to be the major parameter affecting the magnitude of the RMS error. Received: 20 September 2000/Accepted: 2 July 2001 Published online: 29 November 2001     

Micro- and Nanoscale Deformation Measurement of Surface and Internal Planes via Digital Image Correlation

The digital image correlation (DIC) technique is successfully applied across multiple length scales through the generation of a suitable speckle pattern at each size scale. For microscale measurements, a random speckle pattern of paint is created with a fine point airbrush. Nanoscale displacement resolution is achieved with a speckle pattern formed by solution deposition of fluorescent silica nanoparticles. When excited, the particles fluoresce and form a speckle pattern that can be imaged with an optical microscope. Displacements are measured on the surface and on an interior plane of transparent polymer samples with the different speckle patterns. Rigid body translation calibrations and uniaxial tension experiments establish a surface displacement resolution of 1 μm over a 5×6 mm scale field of view for the airbrushed samples and 17 nm over a 100×100 μm scale field of view for samples with the fluorescent nanoparticle speckle. To demonstrate the capabilities of the method, we characterize the internal deformation fields generated around silica microspheres embedded in an elastomer under tensile loading. The DIC technique enables measurement of complex deformation fields with nanoscale precision over relatively large areas, making it of particular relevance to materials that possess multiple length scales.     

The effect of particles on fluid turbulence in a turbulent boundary layer over a cylinder

The turbulent fluid and particle interaction in the turbulent boundary layer for cross flow over a cylinder has been experimentally studied. A phase-Doppler anemometer was used to measure the mean and fluctuating velocities of both phases. Two size ranges of particles (30μm–60μm and 80μm–150μm) at certain concentrations were used for considering the effects of particle sizes on the mean velocity profiles and on the turbulent intensity levels. The measurements clearly demonstrated that the larger particles damped fluid turbulence. For the smaller particles, this damping effect was less noticeable. The measurements further showed a delay in the separation point for two phase turbulent cross flow over a cylinder. The project supported by the National Natural Science Foundation of China     

Scanning Electron Microscopy for Quantitative Small and Large Deformation Measurements Part II: Experimental Validation for Magnifications from 200 to 10,000

A combination of drift distortion removal and spatial distortion removal are performed to correct Scanning Electron Microscope (SEM) images at both ×200 and ×10,000 magnification. Using multiple, time-spaced images and in-plane rigid body motions to extract the relative displacement field throughout the imaging process, results from numerical simulations clearly demonstrate that the correction procedures successfully remove both drift and spatial distortions with errors on the order of ±0.02 pixels. A series of 2D translation and tensile loading experiments are performed in an SEM for magnifications at ×200 and ×10,000, where both the drift and spatial distortion removal methods described above are applied to correct the digital images and improve the accuracy of measurements obtained using 2D-DIC. Results from translation and loading experiments indicate that (a) the fully corrected displacement components have nearly random variability with standard deviation of 0.02 pixels (≈25 nm at ×200 and ≈0.5 nm at ×10,000) in each displacement component and (b) the measured strain fields are unbiased and in excellent agreement with expected results, with a spatial resolution of 43 pixels (≈54 μm at ×200 and ≈1.1 μm at ×10,000) and a standard deviation on the order of 6 × 10⁻⁵ for each component.

Measurement of fully-developed turbulent pipe flow with digital particle image velocimetry

A new and unique high-resolution image acquisition system for digital particle image velocimetry (DPIV) in turbulent flows is used for the measurement of fully-developed turbulent pipe flow at a Reynolds number of 5300. The flow conditions of the pipe flow match those of a direct numerical simulation (DNS) and of measurements with conventional (viz., photographic) PIV and with laser-Doppler velocimetry (LDV). This experiment allows a direct and detailed comparison of the conventional and digital implementations of the PIV method for a non-trivial unsteady flow. The results for the turbulence statistics and power spectra show that the level of accuracy for DPIV is comparable to that of conventional PIV, despite a considerable difference in the interrogation pixel resolution, i.e. 32 × 32 (DPIV) versus 256 × 256 (PIV). This result is in agreement with an earlier analytical prediction for the measurement accuracy. One of the advantages of DPIV over conventional PIV is that the interrogation of the DPIV images takes only a fraction of the time needed for the interrogation of the PIV photographs.     

Holographic particle image velocimetry system for measurements of hairpin vortices in air channel flow

A holographic particle image velocimetry system for investigating hairpin vortices, artificially generated in a subcritical plane Poiseuille air flow, is presented. The optical setup is a modified version of the hybrid scheme, previously employed in turbulent water flows. Accordingly, separate reconstruction of holograms, successively recorded on the same photoplate, is provided by using two reference beams. The positioning of the photoplate within the image of the sample volume accompanied by special alignment procedures, minimizes the apparent displacement caused by the misalignment of the reconstruction waves. A novel method is employed for detecting in-focus particles. Testing the system with a fixed 5 μm diameter wire, results in a corresponding 3D wire image having a diameter of ≈25 μm. Finally, the instantaneous topology and 3D distribution of the two velocity components associated with the hairpin vortex are presented.     

Performance of propellant decomposition products as fuel in an airbreathing PDE

Decomposition products of a solid propellant are considered as a possible fuel in an airbreathing pulse detonation engine (PDE). However, these decomposition products contain not only gaseous species but also a significant amount of solid carbon particles. Whether performance can be improved by burning these particles is investigated numerically. Thermodynamic calculations allow predicting the quantity of additional air required for optimum performance. Gasdynamic numerical simulations indicate that particle burning has an effect on the pressure impulse on the thrust wall. The particle size determines the detonation structure, according to the model of hybrid detonations, thus governing the delay and rate of heat release from particle combustion behind the detonation front. In the situation investigated here, the particles are incompletely burnt inside a 0.6-m-long tube. As a result, smaller particles ( ≤ 5μm) contribute to an increase in the impulse, by up to 6%. However, larger particles either have a negligible effect on the pressure impulse, if around 10 μm, or result in a decrease, if around 20 μm. Overall, the calculations show that the best efficiency is obtained for this fuel by diluting the gaseous decomposition products with an additional quantity of air, allowing for incomplete particle combustion rather than letting them behave as if inert, absorbing part of the energy released by gaseous combustion.This paper was based on a work that was presented at the 20th International Colloquium on the Dynamics of Explosions and Reactive Systems, Montreal, Canada, July 31–August 5, 2005.     

Microscopic high-speed liquid-metal jets in vacuum

The operation of microscopic high-speed liquid-metal jets in vacuum has been investigated. We show that such jets may be produced with good stability and collimation at higher speeds than previously demonstrated, provided that the nozzle design is appropriate and that cavitation-induced instabilities are avoided. The experiments with a medium-speed tin jet (u ∼ 60 m/s, Re=1.8×10⁴, Z=2.9×10⁻³) showed that it operated without any signs of instabilities, whereas the stability of high-speed tin jets (d=30 μm, u=500 m/s, Re=5.6×10⁴, Z=4.7×10⁻³) has been investigated via dynamic similarity using a water jet. Such a 500-m/s tin jet is required as the anode for high-brightness operation of a novel electron-impact X-ray source.