陕滇两地天麻中天麻素的含量比较

采用HPLC法测定天麻中天麻素的含量,分析比较陕滇两地天麻中天麻素含量,筛选优质药材。色谱条件:安捷伦-C18色谱柱(4.6mm×250mm,5μm);流动相为乙腈-0.05%磷酸溶液(3∶97);流速为1.0mL.min-1;检测波长为220nm;柱温为25℃。结果表明,陕滇两地天麻中天麻素含量差异明显,陕西产天麻中天麻素含量高于云南,陕西为0.344%,云南为0.287%。     

高效毛细管电泳法测定全天麻胶囊中天麻素的含量

建立了利用毛细管电泳技术测定全天麻胶囊中天麻素含量的新方法.所用熔融石英毛细管规格为50μm×60cm(有效长度为45cm),检测波长为220nm,分离电压为12kV,缓冲液为pH7.6的200mmol/L硼酸缓冲溶液.结果表明,天麻素在10-800μg/mL范围内线性关系良好(r=0.9995),加样回收率为98.5...     

RP-HPLC测定黄毛楤木中的紫丁香苷和刺五加苷E

研究RP-HPLC同时定量分析黄毛楤木中紫丁香苷和刺五加苷E的含量。采用色谱柱为NUCLEODUR 100-5 C18ec柱(4.6mm×250mm,5μm),流动相为乙腈∶水(15∶85,V/V),流速0.8mL.min-1,检测波长221nm,柱温30℃。紫丁香苷进样量在0.01364—0.15004μg范围内,与峰面积呈良好的线性关系(r=0.9999),平均回收率为96.8%,RSD为2.5%(n=6);刺五加苷E进样量在0.02940—0.32340μg范围内,与峰面积呈良好的线性关系(r=0.9999),平均回收率为97.9%,RSD为1.9%(n=6)。本方法操作简便,测定结果准确可靠,可同时测量黄毛楤木中紫丁香苷和刺五加苷E的含量。     

高效液相色谱法测定枸杞叶粗蛋白中的活性多肽IN-5

建立高效液相色谱法测定枸杞叶粗蛋白中活性多肽IN-5含量的方法.色谱条件为:色谱柱Kinetex C18 (250mm×4.6mm,2.6μm);流动相为乙腈-0.2％磷酸水(5∶95,V/V);检测波长210nm;流速为0.7mL/min;柱温25℃.结果表明,IN-5浓度在16.0-240.0μg/mL内与峰面积的线性关系良好(r=0.9999).平均回收率99.2％,RSD=0.49％(n=9).方法快速、准确、重复性好,可用于IN-5含量的检测.     

RP-HPLC同时测定白花檵木花中的槲皮素和山奈酚

建立用反相高效液相色谱法同时测定白花檵木花中槲皮素和山奈酚含量的方法。采用Eclipse XDB-C18色谱柱(4.6mm×250mm,5μm);柱温:25℃;流动相为乙腈-0.4%磷酸水溶液(35:65,V/V);流速为1.0mL·min-1;检测波长为360nm。槲皮素在1.08×10-3—1.08×10-1μg·μL-1的范围内具有良好的线性关系(r=0.9999),平均回收率97.23%,RSD为1.26%;山奈酚在1.04×10-3—1.04×10-1μg.μL-1的范围内具有良好的线性关系(r=0.9999),平均回收率99.78%,RSD为0.46%。方法简便、准确、可靠,可作为白花檵木花中槲皮素和山奈酚的定量分析方法。     

RRLC测定葡萄籽超临界CO2萃取液中的原花青素

用高分离度快速分离液相色谱法测定超临界葡萄籽萃取液中原花青素含量。色谱柱:ZorbaxXDB C18(4.6mm×50mm,1.8μm),柱温:40℃;流动相:1.5%乙酸甲醇-1.5%乙酸水(90:10,V/V);检测波长:280nm;进样量:5μL,流速:0.9mL/min。原花青素在0.60—3.6mg/mL(R2=0.9997)范围内峰面积与浓度呈良好线性关系,平均加标回收率为100.6%,RSD=1.07%(n=6)。方法简单、快速、准确、可靠,为原花青素含量测定提供一种新选择。     

兔粪便中多烯紫杉醇的测定

建立了一种反相高效液相色谱法测定兔粪中多烯紫杉醇含量的方法。采用乙醚为萃取剂,液相色谱条件为:Phenomenex LUNA C18色谱柱(4.6mm×250mm,5μm);流动相为甲醇-乙腈-水(42:26:32,V/V/V);紫杉醇为内标;流速:1.0mL/min;检测波长:230nm;柱温:26℃。在浓度为1.38—55.0μg/g范围内,峰面积与浓度之间线性关系良好,相关系数为0.9993,平均回收率大于85.0%,相对标准偏差小于10.00%。结果表明,该方法具有操作简便、精密度和准确度较高的特点,可用于兔粪中多烯紫杉醇含量的测定。     

HPLC测定川菊中绿原酸的含量

建立川菊中绿原酸含量的检测方法。采用高效液相色谱法,以绿原酸为指标,DiamonsilTMC18柱(4.6mm×150mm,5μm);0.1mol·L-1的NaH2PO4-CH3OH(7.3∶2.7)为流动相,检测波长为326nm。平均回收率为101.5%,RSD=4.8%。进样量在0.092—0.552μg,线性关系良好,r=0.9989。该方法简便、稳定、重现性好,为菊花质量的控制提供参考。     

RRLC测定关黄柏中盐酸小檗碱的含量

采用超声波法提取关黄柏中有效成分盐酸小檗碱,应用正交试验法筛选盐酸小檗碱最佳提取工艺。采用高分离度快速液相色谱(RRLC)测定关黄柏中有效成分盐酸小檗碱的含量。色谱条件为:色谱柱:XDB-C18(4.6mm×50mm,1.8μm);流动相:乙腈-0.3%磷酸缓冲溶液(29∶71);检测波长:266nm;流速:0.7mL/min;柱温:25℃;进样量:2μL。盐酸小檗碱在0.075—0.3μg范围内线性关系良好,r=0.9998,其平均回收率为99.2%,RSD为1.48%。本法操作快速简便、准确可靠,适用于关黄柏中盐酸小檗碱的含量测定。     

高效液相色谱法测定补肾益寿胶囊中的淫羊藿苷

建立高效液相色谱法测定补肾益寿胶囊中淫羊藿苷的含量。色谱条件为DiamonsilTMC18(250mm×4.6mm,5μm)色谱柱,以乙腈-水(30∶70,V/V)为流动相,检测波长270nm,柱温25℃。淫羊藿苷在0.0842—1.2630μg范围内线性关系良好(r=0.9992),平均回收率为99.4%(RSD为0.76%,n=6)。结果表明,所建立的高效液相色谱法适用于补肾益寿胶囊中淫羊藿苷含量的测定。     

Guided-Wave Two-Dimensional Acousto-Optic Scanner Using Proton-Exchanged Lithium Niobate Waveguide

Two-dimensional scanning of a 0.6328 mum guided-light beam has been realized using noncolinear acousto-optic (AO) coplanar Bragg diffraction together with colinear AO guided-mode to substrate radiation-mode conversion in a Z-cut Xpropagation LiNbO3 proton-exchanged (PE) waveguide. The two surface acoustic (SAW) waves utilized are at the center frequencies of 500 and 200 MHz, propagating in the Y and X axes, respectively. Two-dimensional scanning of approximately 720 resolvable light beam spots, namely, 18 40 (horizontal vertical) scanning, has been demonstrated using a light beam of 1.0 mm aperture. The total number of resolvable beam spots can be greatly increased from 720 by simply utilizing SAW transducers of larger bandwidth and a light beam of greater aperture. It should also be possible to significantly increase the diffraction efficiency from 3 % by optimizing the parameters of the PE waveguide and the SAWs.     

Hybrid Wavelength and Code Division Multiple Access in Optical Networks

A review of schemes for multiple access in fiber optic networks shows that a hybrid of wavelength and code division multiple access (WCDMA) combines the best features of both. In particular, the hybrid scheme retains the large information carrying capacity of wavelength division multiple access (WDMA) and flexibility of code division multiple access (CDMA). In this paper WDMA, optical CDMA (OCDMA), and WCDMA networks are discussed. In OCDMA networks, concept of incoherent and coherent coding including inverse decoding and matched filter is introduced. The delay performance of both WDMA and WCDMA networks, under the simple suboptimum access protocols based on cyclic search, is computed. It has been shown quantitatively that tuning delay significantly affects the delay performance of both WDMA and WCDMA networks. Futhermore, delay performance of WCDMA networks is always better than the WDMA networks for the same tuning delay, load, and number of users.     

Effect ofγ-radiation on the spectral luminescence characteristics of impure cadmium tungstate crystals

Results of a study of the effect of γ-radiation on the spectral luminescence properties of cadmium tungstate crystals doped with silver, bismuth, and molybdenum cations are presented. Spectral characteristics of the nondnnnoped crystals are briefly described. Absorption and photo and X-ray luminescence spectra of the crystals taken before and after exposure to γ-radiation (5.5·10⁴ Gy) are compared. It is found that the spectral characteristics of the crystals doped with silver, bismuth, and molybdenum cations do not change markedly after the exposure. The relation between the type of impurity-induced defects, individual characteristics of the impurity cations, and the character of the effect of γ-radiation on the spectral luminescence properties of impure crystals is analyzed (preliminarily). Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 64, No. 1, pp. 55–60, January–February, 1997.     

Theoretical analysis and simulation of conjugate heights for dual-conjugate AO system in lidar

A multi-conjugate adaptive optics （MCAO） can offer a possibility of widening field of view （FOV） characterized by the isoplanatic angle, and the choose of conjugate height becomes a basic problem for MCAO, which influences the size of iosplanatic angle. Considering the application of lidar, the isoplanatic angle＇s expressions of two deformable mirrors （DMs） MCAO for uplink and downlink are deduced. The effects of conjugate heights for dual-conjugate AO are thoughtfully discussed, and the isoplanatic angles are further analyzed. The results show that the isopanatic angle varies with the conjugate height and reaches the maximum as the conjugate height is at the optimal altitude. Moreover, the optimal conjugate height changes with the propagation distance.     

Quick single-photon detector with many avalanche photo diodes working on the time division

Due to the limit of response speed of the present single-photon detector, the code rate is still too low to come into practical use for the present quantum key distribution (QKD) system.A new idea is put up to design a quick single-photon detector.This quick single-photon detector is composed of a multi-port optic-fiber splitter and many avalanche photo diodes (APDs).Au of the ports with APDs work on the time division and cooperate with a logic discriminating and deciding unit driven by the clock signal.The operation frequency lies on the number N of ports, and can reach N times of the conventional single-photon detector.The single-photon prompt detection can come true for high repetition-rate pulses.The applying of this detector will largely raise the code rate of the QKD, and boost the commercial use.     

Research on beamforming method for dual-line array with space mismatch between the two line arrays

The performance of port/starboard discrimination of dual-line array will be seriously degraded when the space between the two line arrays of the dual-line array is mismatched. The directivity function of the delay-sum and delay-subtract beamforming is deduced and the port/starboard discrimination of the delay-sum and delay-subtract beamforming is also studied both under the ideal status and the status under which the space between the two line arrays of the dual-line array is mismatched. Analysis results show the delay-subtract beamforming has better performance of port/starboard discrimination. At last, the sea trial results demonstrate the theoretical results.     

Retrieval of ultraviolet skylight radiances and O3 slant column densities from balloon-borne limb spectrometer

With a novel light-weight and absolutely calibrated ultraviolet （UV） spectrometer, UV skylight radiances and O3 slant column densities are measured by balloon-borne limb measurements in Xinjiang area, China. UV skylight radiances measured at the height of 31 km are compared with the results from Modtran in the wavelength range from 290 to 420 nm. O3 slant column densities are derived from radiance spectra in the Huggins bands （320 - 335 nm） using differential optical absorption spectroscopy method. And the parameter exhibits a good correlation with the same value simulated by radiative transfer model （Tracy）. The O3 profile simultaneously measured by an O3 sonde is used as input in Tracy calculations. The O3 sonde is launched on the same balloon.     

Design optimization of highly sensitive LSPR enhanced surface plasmon resonance biosensors with nanoholes

For breaking through the sensitivity limitation of conventional surface plasmon resonance (SPR) biosensors, novel highly sensitive SPR biosensors with Au nanoparticles and nanogratings enhancement have been proposed recently.But in practice, these structures have obvious disadvantages.In this study, a nanohole based sensitivity enhancement SPR biosensor is proposed and the influence of different structural parameters on the performance is investigated by using rigorous coupled wave analysis (RCWA).Electromagnetic field distributions around the nanohole are also given out to directly explain the performance difference for various structural parameters.The results indicate that significant sensitivity increase is associated with localized surface plasmons (LSPs) excitation mediated by nanoholes.Except to outcome the weakness of other LSP based biosensors, larger resonance angle shift, reflectance amplitude, and sharper SPR curves' width are obtained simultaneously under optimized structural parameters.     

Polymer gratings based on photopolymerization for low-order distributed feedback polymer lasers

Novel polymer distributed feedback(DFB)gratings are fabricated based on photopolymerization to reduce lasing threshold of polymer lasers.A photopolymer formulation sensitive to 355-nm ultraviolet(UV)light is proposed for the fabrication of polymer gratings and it can be used to form polymer films by spin-coating process.A very low surface-relief depth ranging from 12.5 to about 1.0 nm has been demonstrated with a refractive-index modulation of about 0.012.The experimental results indicate that such polymer gratings have promising potentials for the fabrication of low-order DFB organic semiconductor lasers.