RP-HPLC同时测定白花檵木花中的槲皮素和山奈酚

建立用反相高效液相色谱法同时测定白花檵木花中槲皮素和山奈酚含量的方法。采用Eclipse XDB-C18色谱柱(4.6mm×250mm,5μm);柱温:25℃;流动相为乙腈-0.4%磷酸水溶液(35:65,V/V);流速为1.0mL·min-1;检测波长为360nm。槲皮素在1.08×10-3—1.08×10-1μg·μL-1的范围内具有良好的线性关系(r=0.9999),平均回收率97.23%,RSD为1.26%;山奈酚在1.04×10-3—1.04×10-1μg.μL-1的范围内具有良好的线性关系(r=0.9999),平均回收率99.78%,RSD为0.46%。方法简便、准确、可靠,可作为白花檵木花中槲皮素和山奈酚的定量分析方法。

Simultaneous Determination of Quercetin and Kaempferol in Flowers of Loropetalum Chinensis(R.Br.) Oliv.by RP-HPLC

A method for the simultaneous determination of the contents of quercetin and kaempferol in the flower of Loropetalum Chinensis(R.Br.) Oliv.by RP-HPLC was established.The compounds were separated by Eclipse XDB-C18(4.6mm×250mm,5μm)column at 25℃ with a mobile phase consisting of acetonitrile-0.4% phosphoric acid solution(35∶65,V/V) at a flow rate of 1.0mL·min-1.The detection wavelength was at 360nm.Quercetin showed a good linear relationship(r=0.9999) in the range of 1.08×10-3—1.08×10-1μg·μL-1,the average recovery was 97.23% with RSD of 1.26%(n=6).Kaempferol showed a good linear relationship(r=0.9999) in the range of 1.04×10-3—1.04×10-1μg·μL-1,the average recovery was 99.78% with RSD of 0.46%(n=6).The developed method is simple,accurate and reliable with good repeatability,and can be used as quantitative analysis of quercetin and kaempferod in the flowers of Loropetalum Chinensis(R.Br.) Oliv..