Interstitial Doppler optical coherence tomography

Doppler optical coherence tomography (OCT) can image tissue structure and blood flow at micrometer-scale resolution but has limited imaging depth. We report a novel, linear-scanning, needle-based Doppler OCT system using angle-polished gradient-index or ball-lensed fibers. A prototype system with a 19-guage (diameter of approximately 0.9 mm) echogenic needle is constructed and demonstrates in vivo imaging of bidirectional blood flow in rat leg and abdominal cavity. To our knowledge, this is the first demonstration of Doppler OCT through a needle probe in interstitial applications to visualize deeply situated microcirculation.     

Feasibility study of phase-sensitive imaging based on multiple reference optical coherence tomography

Multiple reference optical coherence tomography(MR-OCT) is a recently developed, low-cost and compact time-domain OCT solution for primary care and consumer level applications. A combination of a voice coil actuator and a partial mirror(PM) extends the scan range for imaging depths of approximately 1 mm in biological samples. Our previous research on MR-OCT is based only on intensity information obtained from the depth-resolved interference signal. In this Letter, we extract the phase information from the MR-OCT signal and, hence, provide an additional contrast modality. The phase sensitivity of the system is measured to be approximately 0.2 and 1.5 rad for the first and twelfth orders of reflection when using a mirror as the sample.This Letter describes first results of phase-sensitive data measured on a phantom obtained with MR-OCT. Data from a chick embryo chorioallantoic membrane(CAM) is used to demonstrate the feasibility of MR-OCT for in vivo phase-sensitive imaging.     

Endoscopic Optical Doppler Tomography Based on Two-Axis Scanning MEMS Mirror

We study the feasibility of endoscopic optical Doppler tomography with a micro-electro-mechanical system(MEMS) mirror based probe. The additional phase shifts introduced by the probe are tracked and formulated.The suppression method of the probe phase shifts is proposed and validated by fluid flow detection experiments.In vivo blood flow detection is also implemented on a hairless mouse. The velocities of the blood flow in two directions are obtained to be-8.1 mm/s and 6.6 mm/s, respectively.     

基于2D微电子机械系统(MEMS)镜全向激光雷达光学系统设计

为了满足全向激光探测的需求,提出一种基于2DMEMS镜扫描的激光雷达结构。激光器通过1×6高速光开关分时地给6个扫描子系统提供光信号,6个扫描子系统探测视场叠加起来可实现360°激光探测。每个扫描子系统的扫描范围为60°×30°,其中包含一个扩展MEMS镜扫描角度的发射光学天线和一个大视场有增益的接收光学天线。发射光学天线将MEMS镜±10°的扫描角扩展到±30°,发散度小于0.2mrad;接收视场内的激光波经过接收天线在探测器上所成的半像高小于1mm,接收增益为3.65。通过计算修正后的激光雷达方程可得到发射功率20 W的激光束在工作距离100 m内的回波功率≥1 nW,结果表明该光学系统可适用于激光雷达系统。     

Distance sensing using dynamic speckles formed by micro-electro-mechanical-systems deflector

We present a novel architecture of dynamic-speckles distance sensors with a micro-electro-mechanical system (MEMS) deflecting mirror for surface scanning. Since the MEMS mirror deflects the beam sinusoidally, the speed of scanning varies during the scan leading to sufficient variations in the signal frequency which is used to evaluate the distance to the object. Nevertheless, this feature does not affect the accuracy of range measurements when the distance is estimated from zero-crossing counts of the signal during a specially chosen time window within the scan. Experimental results indicate that a single 1 ms scan yields 70 μm accuracy of distance measurement. Averaging data from multiple scans of different surface areas during a 1-s measurement can provide approximately 2 mm of accuracy. Precision of the measurements can be further improved by optimization of the optical system. The compactness of the MEMS deflector and simplicity of the suggested signal processing provides a good basis for practical applications of this distance sensor.     

High-speed scanning fiber-optic heterodyne interferometer using a micro-electro-mechanical system mirror and an f-theta lens

This paper describes a novel fiber-coupled heterodyne interferometer using a micro-electro-mechanical system (MEMS) mirror and an f-theta lens. In this interferometer system, the cross-polarized laser beams operating at 2 μm with the frequency difference of 100 kHz are introduced by means of the two acousto-optic modulators (AOM). The sample with diameter of 300 mm is uniformly scanned by the 1 mm laser beam with the help of the combined optical scanning configuration, a MEMS mirror and an f-theta lens. The output intermediate signal from the two channels, reference channel and the measurement channel, are processed in the 12 bit analog-to-digital (A/D) process system. Details of our interferometer scheme are discussed.     

Linear optical coherence tomography system with a downconverted fringe pattern

Linear optical coherence tomography (LOCT) systems are a simple and robust alternative to time-domain optical coherence tomography systems, but a detector with approximately 10(4) pixels is needed for an imaging depth of 2 mm. We present a new system for LOCT with a special mask attached to the image sensor. The mask essentially performs a downconversion of the spatial frequencies by multiplication with a second spatial frequency. This reduces the fringe frequency of the optical coherence tomography signal so that the signal can be sampled with fewer pixels.     

基于MEMS微镜的长波近红外光谱仪的设计与实现

为适应光谱仪微型化、集成化的发展趋势,详细分析了MEMS微镜应用于微型长波近红外光谱仪的方法和涉及的主要问题,例如分光系统的设计、MEMS微镜的选择、探测器与前置放大电路的设计等。并将50 Hz谐振频率、峰峰驱动电压为10V的MEMS微镜、高灵敏度的InGaAs单元探测器,结合立特罗式分光光路,设计和实现了900～2 055 nm波段的微型长波近红外光谱仪样机,其中1 000～1 965 nm谱段的光谱分辨率介于9.4～16 nm之间。采用MEMS扫描微镜技术后,一方面简化了光谱仪中的复杂机械结构,使尺寸可以更小;另一方面实现了单探测器的长波近红外光谱仪,与阵列长波近红外探测器光谱仪相比,成本有所降低。作为应用实例,此样机成功对纯水以及乙醇-水溶液的长波近红外光谱进行了测量,实现了乙醇-水溶液的浓度预测分析,其中本样机测量的纯水长波近红外光谱与文献相符。     

Forward imaging OCT endoscopic catheter based on MEMS lens scanning

This Letter reports a fully packaged microelectromechanical system (MEMS) endoscopic catheter for forward imaging optical coherence tomography (OCT). Two-dimensional optical scanning of Lissajous patterns was realized by the orthogonal movement of two commercial aspherical glass lenses laterally mounted on two resonating electrostatic MEMS microstages at low operating voltages. The MEMS lens scanner was integrated on a printed circuit board and packaged with an aluminum housing, a gradient index fiber collimator, and an objective lens. A home-built spectral-domain OCT system with 60 kHz A-line acquisition rate was combined with the endoscopic MEMS catheter. Three-dimensional images of 256×256×995 voxels were directly reconstructed by mapping the A-line datasets along the Lissajous patterns. The endoscopic catheter can provide a new direction for forward endoscopic OCT imaging.     

Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two- dimensional scanning mirror

Towards overcoming the size limitations of conventional two-photon fluorescence microscopy, we introduce two-photon imaging based on microelectromechanical systems (MEMS) scanners. Single crystalline silicon scanning mirrors that are 0.75 mm x 0.75 mm in size and driven in two dimensions by microfabricated vertical comb electrostatic actuators can provide optical deflection angles through a range of approximately16 degrees . Using such scanners we demonstrated two-photon microscopy and microendoscopy with fast-axis acquisition rates up to 3.52 kHz.     

Quantification of a three-dimensional velocity vector using spectral-domain Doppler optical coherence tomography

Multiangle, fiber-based, spectral-domain Doppler optical coherence tomography with a phase-resolved algorithm is presented to measure three components of an arbitrary velocity vector. A beam divider that divides a probe beam to have five independent viewpoints and path length delays was designed. The divider was inserted into the sampling arm of a Doppler optical coherence tomography system between the collimator and the first galvo mirror of a two-axis galvo scanner. The divider produced five independent D k's (the average difference between the wave vectors of incoming and outgoing beams) after passing through the focusing lens while keeping two-axis scanning capability. After calibration, an unknown velocity vector field inside a microtube was quantified by solving a three-dimensional minimization problem.     

Real-time, high velocity-resolution color Doppler optical coherence tomography

Color Doppler optical coherence tomography (CDOCT) is a noninvasive optical imaging technique for micrometer-scale physiological flow mapping simultaneously with morphological optical coherence tomography imaging. We have developed a novel CDOCT signal-processing strategy capable of imaging physiological flow rates at 8 frames/s. Our new strategy features hardware-implemented digital autocorrelation across subsequent scans, permitting us to measure 300-Hz-8-kHz Doppler shifts upon signals of 0.6-MHz bandwidth. The performance of the CDOCT system was demonstrated in a flow phantom and in vivo in Xenopus laevis.     

共口径双通道红外扫描成像光学系统

对双波段红外扫描成像光学系统进行了研究,结合三次成像技术和100%冷光栏效率技术,设计了一个共口径双通道红外扫描成像光学系统。该系统包括前端共用的双反射系统、分束镜、准直镜组、扫描镜和成像镜组。光波经过双反射系统在主镜之后被分束镜分成中波红外通道（3 m～5 m）和长波红外通道（10 m～12 m）,经准直镜组及成像镜组会聚探测器上,实现中波红外系统与长波红外系统共口径同步成像。设计结果表明,长波红外系统传递函数在18 lp/mm处达到0.4以上,中波红外系统传递函数在18 lp/mm处达到0.78以上,满足实际应用的要求。     

In vivo bidirectional color Doppler flow imaging of picoliter blood volumes using optical coherence tomography

We describe a novel optical system for bidirectional color Doppler imaging of flow in biological tissues with micrometer-scale resolution and demonstrate its use for in vivo imaging of blood flow in an animal model. Our technique, color Doppler optical coherence tomography (CDOCT), performs spatially localized optical Doppler velocimetry by use of scanning low-coherence interferometry. CDOCT is an extension of optical coherence tomography (OCT), employing coherent signal-acquisition electronics and joint time-frequency analysis algorithms to perform flow imaging simultaneous with conventional OCT imaging. Cross-sectional maps of blood flow velocity with <50-microm spatial resolution and <0.6-mm/s velocity precision were obtained through intact skin in living hamster subdermal tissue. This technology has several potential medical applications.     

基于激光差动多普勒效应的微机电系统动态测试技术

提出了一种用于测量微机电系统(MEMS)器件瞬时速度、位移的测量系统。采用激光差动多普勒技术,检测谐振器在平面内的振动,测量垂直于系统测量光轴方向的振动速度。并在差动多普勒测量光路的基础上加入了CCD监测光路,实时观察被测器件调整过程和振动情况。通过处理电路从光学系统输出的高频信号中提取多普勒信号,利用Labview和Matlab软件对采集的多普勒信号进行时频分析,得到被测器件的运动参量。通过对测点的微定位,对MEMS器件进行整平面的扫描,得到器件振动的瞬时速度场,为进一步对MEMS器件的高阶谱振动分析及扭转分析提供了一定的基础和必要的支持。     

Endoscopic optical coherence tomography based on a microelectromechanical mirror

An endoscopic optical coherence tomography (OCT) system based on a microelectromechanical mirror to facilitate lateral light scanning is described. The front-view OCT scope, adapted to the instrument channel of a commercial endoscopic sheath, allows real-time cross-sectional imaging of living biological tissue via direct endoscopic visual guidance. The transverse and axial resolutions of the OCT scope are roughly 20 and 10.2mum, respectively. Cross-sectional images of 500x1000 pixels covering an area of 2.9 mmx2.8 mm can be acquired at ~5 frames/s and with nearly 100-dB dynamic range. Applications in thickness measurement and bladder tissue imaging are demonstrated.     

利用镜面形变实现共轴折反射式变焦光学系统设计

在光学系统中加入2个或多个可变光学元件,保持光学元件位置不变,通过微调装置改变这些可变光学元件的焦距使得整个光学系统的有效焦距发生变化。基于该设计思想,结合卡塞格林(Cassegrain)反射式望远镜结构模式,使用ZEMAX光学设计软件设计了焦距为1 600 mm～800 mm,视场0.6°～1.2°的变焦系统,整个系统由2个可变形反射面、1个平面反射面和1个透镜组成,主要通过主镜和次镜面型曲率(可变形镜DMs,Deformable Mirrors)以及入瞳直径的变化实现系统变焦。设计结果表明:系统在空间频率16 lp/mm处调制传递函数大于0.75,最大均方根弥散斑半径均小于探测元尺寸,满足成像要求。     

Gaussian beam coupling on a MEMS mirror array

A general formula for evaluating Gaussian beam coupling characteristics on a MEMS mirror array was developed, with consideration of parameters such as beam size, mirror width and mirror gap. Based on the formula, the influence of each parameter on the spectral response was simulated for MEMS mirror array employed in a wavelength selective switch and a tunable optical filter. The simulation results are helpful for the design of MEMS mirror array in optical devices.     

Real-time phase-resolved functional optical coherence tomography by use of optical Hilbert transformation

We have developed a novel real-time phase-resolved functional optical coherence tomography system that uses optical Hilbert transformation. When we use a resonant scanner in the reference arm of the interferometer, with an axial scanning speed of 4 kHz, the frame rate of both structural and Doppler blood-flow imaging with a size of 100 by 100 pixels is 10 Hz. The system has high sensitivity and a larger dynamic range for measuring the Doppler frequency shift that is due to moving red blood cells. Real-time images of in vivo blood flow in human skin obtained with this interferometer are presented.     

Wide dynamic range detection of bidirectional flow in Doppler optical coherence tomography using a two-dimensional Kasai estimator

We demonstrate extended axial flow velocity detection range in a time-domain Doppler optical coherence tomography (DOCT) system using a modified Kasai velocity estimator with computations in both the axial and transverse directions. For a DOCT system with an 8 kHz rapid-scanning optical delay line, bidirectional flow experiments showed a maximum detectable speed of >56 cm/s using the axial Kasai estimator without the occurrence of aliasing, while the transverse Kasai estimator preserved the approximately 7 microm/s minimum detectable velocity to slow flow. By using a combination of transverse Kasai and axial Kasai estimators, the velocity detection dynamic range was over 100 dB. Through a fiber-optic endoscopic catheter, in vivoM-mode transesophageal imaging of the pulsatile blood flow in rat aorta was demonstrated, for what is for the first time to our knowledge, with measured peak systolic blood flow velocity of >1 m/s, while maintaining good sensitivity to detect aortic wall motion at <2 mm/s, using this 2D Kasai technique.